Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy Cows
Lameness in dairy cows is an extremely painful multifactorial condition that affects the welfare of animals and economically impacts the dairy industry worldwide. The aim of this study was to determine the profile of cytokines in the spinal cord dorsal horn of dairy cows with painful chronic inflamm...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-02-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2020.00125/full |
| _version_ | 1811268578214674432 |
|---|---|
| author | Daniel Herzberg Pablo Strobel Alfredo Ramirez-Reveco Marianne Werner Hedie Bustamante |
| author_facet | Daniel Herzberg Pablo Strobel Alfredo Ramirez-Reveco Marianne Werner Hedie Bustamante |
| author_sort | Daniel Herzberg |
| collection | DOAJ |
| description | Lameness in dairy cows is an extremely painful multifactorial condition that affects the welfare of animals and economically impacts the dairy industry worldwide. The aim of this study was to determine the profile of cytokines in the spinal cord dorsal horn of dairy cows with painful chronic inflammatory lameness. Concentrations of 10 cytokines were measured in the spinal cord of seven adult dairy cows with chronic lameness and seven adult dairy cows with no lameness. In all cows lameness was evaluated using a mobility scoring system and registered accordingly. Immediately after euthanasia the spinal cord was removed and 20 cm of lumbar segments (L2–L5) were obtained. After dorsal horn removal and processing, cytokine quantification of tumor necrosis factor-alpha (TNF-α), interleukin-1alpha (IL-1α), interleukin 13 (IL-13), chemokine-10 (CXCL10/IP-10), chemokine-9 (CXCL9/MIG), interferon-alpha (IFN-α), interferon-gamma (IFN-γ), interleukin-21 (IL-21), interleukin-36ra (IL-36ra), and macrophage inflammatory protein-1 beta (MIP-1β) was performed using a multiplex array. Lame cows had higher concentrations of TNF-α, IL-1-α, IL-13, CXCL10, CXCL9, IFN-α, and IFN-γ in their dorsal horn compared to non-lame cows, while IL-21 concentration was decreased. No differences in IL-36ra and MIP-1β concentrations between lame and non-lame cows were observed. Painful chronic inflammation of the hoof in dairy cows leads to a marked increase in cytokine concentration in the dorsal horn of the spinal cord, which could represent a state of neuroinflammation of the Central Nervous System (CNS). |
| first_indexed | 2024-04-12T21:25:23Z |
| format | Article |
| id | doaj.art-2430bfd59fbd433c802618be6ba1fb20 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-12T21:25:23Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-2430bfd59fbd433c802618be6ba1fb202022-12-22T03:16:11ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-02-01710.3389/fvets.2020.00125510509Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy CowsDaniel Herzberg0Pablo Strobel1Alfredo Ramirez-Reveco2Marianne Werner3Hedie Bustamante4Faculty of Veterinary Sciences, Graduate School, Universidad Austral de Chile, Valdivia, ChileFaculty of Veterinary Sciences, Animal Science Institute, Universidad Austral de Chile, Valdivia, ChileFaculty of Veterinary Sciences, Animal Science Institute, Universidad Austral de Chile, Valdivia, ChileFaculty of Veterinary Sciences, Animal Science Institute, Universidad Austral de Chile, Valdivia, ChileFaculty of Veterinary Sciences, Veterinary Clinical Sciences Institute, Universidad Austral de Chile, Valdivia, ChileLameness in dairy cows is an extremely painful multifactorial condition that affects the welfare of animals and economically impacts the dairy industry worldwide. The aim of this study was to determine the profile of cytokines in the spinal cord dorsal horn of dairy cows with painful chronic inflammatory lameness. Concentrations of 10 cytokines were measured in the spinal cord of seven adult dairy cows with chronic lameness and seven adult dairy cows with no lameness. In all cows lameness was evaluated using a mobility scoring system and registered accordingly. Immediately after euthanasia the spinal cord was removed and 20 cm of lumbar segments (L2–L5) were obtained. After dorsal horn removal and processing, cytokine quantification of tumor necrosis factor-alpha (TNF-α), interleukin-1alpha (IL-1α), interleukin 13 (IL-13), chemokine-10 (CXCL10/IP-10), chemokine-9 (CXCL9/MIG), interferon-alpha (IFN-α), interferon-gamma (IFN-γ), interleukin-21 (IL-21), interleukin-36ra (IL-36ra), and macrophage inflammatory protein-1 beta (MIP-1β) was performed using a multiplex array. Lame cows had higher concentrations of TNF-α, IL-1-α, IL-13, CXCL10, CXCL9, IFN-α, and IFN-γ in their dorsal horn compared to non-lame cows, while IL-21 concentration was decreased. No differences in IL-36ra and MIP-1β concentrations between lame and non-lame cows were observed. Painful chronic inflammation of the hoof in dairy cows leads to a marked increase in cytokine concentration in the dorsal horn of the spinal cord, which could represent a state of neuroinflammation of the Central Nervous System (CNS).https://www.frontiersin.org/article/10.3389/fvets.2020.00125/fulldairy cowslamenesschronic paincytokinespinal cord |
| spellingShingle | Daniel Herzberg Pablo Strobel Alfredo Ramirez-Reveco Marianne Werner Hedie Bustamante Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy Cows Frontiers in Veterinary Science dairy cows lameness chronic pain cytokine spinal cord |
| title | Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy Cows |
| title_full | Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy Cows |
| title_fullStr | Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy Cows |
| title_full_unstemmed | Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy Cows |
| title_short | Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy Cows |
| title_sort | chronic inflammatory lameness increases cytokine concentration in the spinal cord of dairy cows |
| topic | dairy cows lameness chronic pain cytokine spinal cord |
| url | https://www.frontiersin.org/article/10.3389/fvets.2020.00125/full |
| work_keys_str_mv | AT danielherzberg chronicinflammatorylamenessincreasescytokineconcentrationinthespinalcordofdairycows AT pablostrobel chronicinflammatorylamenessincreasescytokineconcentrationinthespinalcordofdairycows AT alfredoramirezreveco chronicinflammatorylamenessincreasescytokineconcentrationinthespinalcordofdairycows AT mariannewerner chronicinflammatorylamenessincreasescytokineconcentrationinthespinalcordofdairycows AT hediebustamante chronicinflammatorylamenessincreasescytokineconcentrationinthespinalcordofdairycows |