Isolation and Genomic Characteristics of Cat-Borne <i>Campylobacter felis</i> sp. nov. and Sheep-Borne <i>Campylobacter ovis</i> sp. nov
Nine novel bacterial strains were isolated from the feces of cats and sheep in 2019 and 2020 in Beijing, China. Cells were 1–3 μm long and ≤0.5 μm wide, Gram-stain negative, microaerobic, motile, oxidase positive, and urease negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated...
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/971 |
| _version_ | 1797604223439863808 |
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| author | Hairui Wang Ying Li Yixin Gu Guilan Zhou Xiaoli Chen Xin Zhang Zhujun Shao Jianzhong Zhang Maojun Zhang |
| author_facet | Hairui Wang Ying Li Yixin Gu Guilan Zhou Xiaoli Chen Xin Zhang Zhujun Shao Jianzhong Zhang Maojun Zhang |
| author_sort | Hairui Wang |
| collection | DOAJ |
| description | Nine novel bacterial strains were isolated from the feces of cats and sheep in 2019 and 2020 in Beijing, China. Cells were 1–3 μm long and ≤0.5 μm wide, Gram-stain negative, microaerobic, motile, oxidase positive, and urease negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated that these nine isolates belong to the genus <i>Campylobacter</i> but formed two robust clades that were clearly separate from the currently recognized species and, respectively, isolated from the cat and sheep. Both these strains shared low 16S rRNA gene sequence similarity, dDDH relatedness, and ANI values with their closest species <i>C. upsaliensis</i> CCUG 14913<sup>T</sup> and <i>C. lanienae</i> NCTC 13004<sup>T</sup>, and against each other, which are below the cut-off values generally recognized for isolates of the same species. The genomic DNA G + C contents of type strains XJK22-1<sup>T</sup> and SYS25-1<sup>T</sup> were 34.99 mol% and 32.43 mol%, respectively. Electron microscopy showed that these cells were spiral shaped, with bipolar single flagella. Based on results from genotypic, phenotypic, phylogenetic, and phylogenomic analyses, these nine strains represent two novel species within the genus <i>Campylobacter</i>, for which the names <i>Campylobacter felis</i> sp. nov. (Type strain XJK22-1<sup>T</sup> = GDMCC 1.3684<sup>T</sup> = JCM 35847<sup>T</sup>) and <i>Campylobacter ovis</i> sp. nov. (Type strain SYS25-1<sup>T</sup> = GDMCC 1.3685<sup>T</sup>) are proposed. |
| first_indexed | 2024-03-11T04:43:17Z |
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| language | English |
| last_indexed | 2024-03-11T04:43:17Z |
| publishDate | 2023-04-01 |
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| series | Microorganisms |
| spelling | doaj.art-24322a30e7274419a4e9b7796e92c2162023-11-17T20:33:10ZengMDPI AGMicroorganisms2076-26072023-04-0111497110.3390/microorganisms11040971Isolation and Genomic Characteristics of Cat-Borne <i>Campylobacter felis</i> sp. nov. and Sheep-Borne <i>Campylobacter ovis</i> sp. novHairui Wang0Ying Li1Yixin Gu2Guilan Zhou3Xiaoli Chen4Xin Zhang5Zhujun Shao6Jianzhong Zhang7Maojun Zhang8State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Rd155, Changbailu, Changping, Beijing 102206, ChinaShunyi District Center for Disease Control and Prevention, Beijing 101320, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Rd155, Changbailu, Changping, Beijing 102206, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Rd155, Changbailu, Changping, Beijing 102206, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Rd155, Changbailu, Changping, Beijing 102206, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Rd155, Changbailu, Changping, Beijing 102206, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Rd155, Changbailu, Changping, Beijing 102206, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Rd155, Changbailu, Changping, Beijing 102206, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Rd155, Changbailu, Changping, Beijing 102206, ChinaNine novel bacterial strains were isolated from the feces of cats and sheep in 2019 and 2020 in Beijing, China. Cells were 1–3 μm long and ≤0.5 μm wide, Gram-stain negative, microaerobic, motile, oxidase positive, and urease negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated that these nine isolates belong to the genus <i>Campylobacter</i> but formed two robust clades that were clearly separate from the currently recognized species and, respectively, isolated from the cat and sheep. Both these strains shared low 16S rRNA gene sequence similarity, dDDH relatedness, and ANI values with their closest species <i>C. upsaliensis</i> CCUG 14913<sup>T</sup> and <i>C. lanienae</i> NCTC 13004<sup>T</sup>, and against each other, which are below the cut-off values generally recognized for isolates of the same species. The genomic DNA G + C contents of type strains XJK22-1<sup>T</sup> and SYS25-1<sup>T</sup> were 34.99 mol% and 32.43 mol%, respectively. Electron microscopy showed that these cells were spiral shaped, with bipolar single flagella. Based on results from genotypic, phenotypic, phylogenetic, and phylogenomic analyses, these nine strains represent two novel species within the genus <i>Campylobacter</i>, for which the names <i>Campylobacter felis</i> sp. nov. (Type strain XJK22-1<sup>T</sup> = GDMCC 1.3684<sup>T</sup> = JCM 35847<sup>T</sup>) and <i>Campylobacter ovis</i> sp. nov. (Type strain SYS25-1<sup>T</sup> = GDMCC 1.3685<sup>T</sup>) are proposed.https://www.mdpi.com/2076-2607/11/4/971<i>Campylobacter felis</i><i>Campylobacter ovis</i>novel speciesgenomic characteristicsphylogenetic analyses |
| spellingShingle | Hairui Wang Ying Li Yixin Gu Guilan Zhou Xiaoli Chen Xin Zhang Zhujun Shao Jianzhong Zhang Maojun Zhang Isolation and Genomic Characteristics of Cat-Borne <i>Campylobacter felis</i> sp. nov. and Sheep-Borne <i>Campylobacter ovis</i> sp. nov Microorganisms <i>Campylobacter felis</i> <i>Campylobacter ovis</i> novel species genomic characteristics phylogenetic analyses |
| title | Isolation and Genomic Characteristics of Cat-Borne <i>Campylobacter felis</i> sp. nov. and Sheep-Borne <i>Campylobacter ovis</i> sp. nov |
| title_full | Isolation and Genomic Characteristics of Cat-Borne <i>Campylobacter felis</i> sp. nov. and Sheep-Borne <i>Campylobacter ovis</i> sp. nov |
| title_fullStr | Isolation and Genomic Characteristics of Cat-Borne <i>Campylobacter felis</i> sp. nov. and Sheep-Borne <i>Campylobacter ovis</i> sp. nov |
| title_full_unstemmed | Isolation and Genomic Characteristics of Cat-Borne <i>Campylobacter felis</i> sp. nov. and Sheep-Borne <i>Campylobacter ovis</i> sp. nov |
| title_short | Isolation and Genomic Characteristics of Cat-Borne <i>Campylobacter felis</i> sp. nov. and Sheep-Borne <i>Campylobacter ovis</i> sp. nov |
| title_sort | isolation and genomic characteristics of cat borne i campylobacter felis i sp nov and sheep borne i campylobacter ovis i sp nov |
| topic | <i>Campylobacter felis</i> <i>Campylobacter ovis</i> novel species genomic characteristics phylogenetic analyses |
| url | https://www.mdpi.com/2076-2607/11/4/971 |
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