Subtherapeutic Kitasamycin Promoted Fat Accumulation in the Longissimus Dorsi Muscle in Growing–Finishing Pigs
Kitasamycin (KM), a broad—spectrum macrolide antibiotic, has implications for growth performance and residue in animals and humans. This study aimed to explore the effects of different KM doses on intramuscular fat accumulation, cecal microflora, and short—chain fatty acids (SCFAs) using a growing–f...
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MDPI AG
2024-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/7/1057 |
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| author | Ge Han Jie Yu Jun He Ping Zheng Xiangbing Mao Bing Yu |
| author_facet | Ge Han Jie Yu Jun He Ping Zheng Xiangbing Mao Bing Yu |
| author_sort | Ge Han |
| collection | DOAJ |
| description | Kitasamycin (KM), a broad—spectrum macrolide antibiotic, has implications for growth performance and residue in animals and humans. This study aimed to explore the effects of different KM doses on intramuscular fat accumulation, cecal microflora, and short—chain fatty acids (SCFAs) using a growing–finishing pig model. Forty—two pigs were divided into three groups: control, subtherapeutic KM (50 mg/kg, KM50), and therapeutic KM (200 mg/kg, KM200) diets over 8 weeks. KM50 led to increased back fat thickness, fat content in the longissimus dorsi muscle (LM), and elevated plasma total cholesterol (TC) levels (<i>p</i> < 0.05), supported by upregulated lipid synthesis gene expression (<i>Acc1</i>, <i>Fas</i>, <i>Scd1</i>) (<i>p</i> < 0.05) in the LM. KM50 altered cecal microflora, reducing <i>Lactobacillus</i> spp. and <i>Bifidobacterium</i> spp. abundance, while increasing SCFA concentrations (acetic acid, propionic acid, total SCFAs) (<i>p</i> < 0.05). KM200 had minimal effects on intestinal weight and density, with increased apparent digestibility of nutrients. These findings highlight the dose-dependent impact of KM on intramuscular fat deposition. Subtherapeutic KM induced ectopic fat deposition, emphasizing potential risks in disease treatment for humans and animals. |
| first_indexed | 2024-04-24T10:50:42Z |
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| id | doaj.art-2442b58c44834ceaabfb53ba849e555c |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-24T10:50:42Z |
| publishDate | 2024-03-01 |
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| series | Animals |
| spelling | doaj.art-2442b58c44834ceaabfb53ba849e555c2024-04-12T13:14:14ZengMDPI AGAnimals2076-26152024-03-01147105710.3390/ani14071057Subtherapeutic Kitasamycin Promoted Fat Accumulation in the Longissimus Dorsi Muscle in Growing–Finishing PigsGe Han0Jie Yu1Jun He2Ping Zheng3Xiangbing Mao4Bing Yu5Key Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Disease-Resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaKitasamycin (KM), a broad—spectrum macrolide antibiotic, has implications for growth performance and residue in animals and humans. This study aimed to explore the effects of different KM doses on intramuscular fat accumulation, cecal microflora, and short—chain fatty acids (SCFAs) using a growing–finishing pig model. Forty—two pigs were divided into three groups: control, subtherapeutic KM (50 mg/kg, KM50), and therapeutic KM (200 mg/kg, KM200) diets over 8 weeks. KM50 led to increased back fat thickness, fat content in the longissimus dorsi muscle (LM), and elevated plasma total cholesterol (TC) levels (<i>p</i> < 0.05), supported by upregulated lipid synthesis gene expression (<i>Acc1</i>, <i>Fas</i>, <i>Scd1</i>) (<i>p</i> < 0.05) in the LM. KM50 altered cecal microflora, reducing <i>Lactobacillus</i> spp. and <i>Bifidobacterium</i> spp. abundance, while increasing SCFA concentrations (acetic acid, propionic acid, total SCFAs) (<i>p</i> < 0.05). KM200 had minimal effects on intestinal weight and density, with increased apparent digestibility of nutrients. These findings highlight the dose-dependent impact of KM on intramuscular fat deposition. Subtherapeutic KM induced ectopic fat deposition, emphasizing potential risks in disease treatment for humans and animals.https://www.mdpi.com/2076-2615/14/7/1057kitasamycinfat accumulationcecal microfloragrowing finishing pigs |
| spellingShingle | Ge Han Jie Yu Jun He Ping Zheng Xiangbing Mao Bing Yu Subtherapeutic Kitasamycin Promoted Fat Accumulation in the Longissimus Dorsi Muscle in Growing–Finishing Pigs Animals kitasamycin fat accumulation cecal microflora growing finishing pigs |
| title | Subtherapeutic Kitasamycin Promoted Fat Accumulation in the Longissimus Dorsi Muscle in Growing–Finishing Pigs |
| title_full | Subtherapeutic Kitasamycin Promoted Fat Accumulation in the Longissimus Dorsi Muscle in Growing–Finishing Pigs |
| title_fullStr | Subtherapeutic Kitasamycin Promoted Fat Accumulation in the Longissimus Dorsi Muscle in Growing–Finishing Pigs |
| title_full_unstemmed | Subtherapeutic Kitasamycin Promoted Fat Accumulation in the Longissimus Dorsi Muscle in Growing–Finishing Pigs |
| title_short | Subtherapeutic Kitasamycin Promoted Fat Accumulation in the Longissimus Dorsi Muscle in Growing–Finishing Pigs |
| title_sort | subtherapeutic kitasamycin promoted fat accumulation in the longissimus dorsi muscle in growing finishing pigs |
| topic | kitasamycin fat accumulation cecal microflora growing finishing pigs |
| url | https://www.mdpi.com/2076-2615/14/7/1057 |
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