Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre‐analytical conditions
Abstract Introduction This study investigated alternative pre‐analytical handling of blood for neurofilament light (NfL) analysis where resources are limited. Method Plasma NfL was measured with single molecule array after alternative blood processing procedures: dried plasma spots (DPS), dried bloo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/dad2.12145 |
| _version_ | 1828085283471491072 |
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| author | Joel Simrén Nicholas J. Ashton Kaj Blennow Henrik Zetterberg |
| author_facet | Joel Simrén Nicholas J. Ashton Kaj Blennow Henrik Zetterberg |
| author_sort | Joel Simrén |
| collection | DOAJ |
| description | Abstract Introduction This study investigated alternative pre‐analytical handling of blood for neurofilament light (NfL) analysis where resources are limited. Method Plasma NfL was measured with single molecule array after alternative blood processing procedures: dried plasma spots (DPS), dried blood spots (DBS), and delayed 48‐hour centrifugation. These were compared to standardized plasma processing (reference standard [RS]). In a discovery cohort (n = 10) and a confirmatory cohort (n = 21), whole blood was obtained from individuals with unknown clinical etiology. In the confirmatory cohort, delayed centrifugation protocol was paired with either 37°C incubation or sample shaking to test the effect of these parameters. Results Delayed centrifugation (R2 = 0.991) and DPS (discovery cohort, R2 = 0.954; confirmatory cohort, DPS: R2 = 0.961) methods were strongly associated with the RS. Delayed centrifugation with higher temperatures (R2 = 0.995) and shaking (R2 = 0.975) did not affect this association. DPS (P < 0.001) returned concentrations considerably lower than the RS. Discussion DPS or delayed centrifugation are viable pre‐analytical procedures for the accurate quantification of plasma NfL. |
| first_indexed | 2024-04-11T04:36:35Z |
| format | Article |
| id | doaj.art-2442ba7479ae43c2bf076e60bf77685e |
| institution | Directory Open Access Journal |
| issn | 2352-8729 |
| language | English |
| last_indexed | 2024-04-11T04:36:35Z |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring |
| spelling | doaj.art-2442ba7479ae43c2bf076e60bf77685e2022-12-28T09:12:13ZengWileyAlzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring2352-87292021-01-01131n/an/a10.1002/dad2.12145Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre‐analytical conditionsJoel Simrén0Nicholas J. Ashton1Kaj Blennow2Henrik Zetterberg3Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy University of Gothenburg Mölndal SwedenDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy University of Gothenburg Mölndal SwedenDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy University of Gothenburg Mölndal SwedenDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy University of Gothenburg Mölndal SwedenAbstract Introduction This study investigated alternative pre‐analytical handling of blood for neurofilament light (NfL) analysis where resources are limited. Method Plasma NfL was measured with single molecule array after alternative blood processing procedures: dried plasma spots (DPS), dried blood spots (DBS), and delayed 48‐hour centrifugation. These were compared to standardized plasma processing (reference standard [RS]). In a discovery cohort (n = 10) and a confirmatory cohort (n = 21), whole blood was obtained from individuals with unknown clinical etiology. In the confirmatory cohort, delayed centrifugation protocol was paired with either 37°C incubation or sample shaking to test the effect of these parameters. Results Delayed centrifugation (R2 = 0.991) and DPS (discovery cohort, R2 = 0.954; confirmatory cohort, DPS: R2 = 0.961) methods were strongly associated with the RS. Delayed centrifugation with higher temperatures (R2 = 0.995) and shaking (R2 = 0.975) did not affect this association. DPS (P < 0.001) returned concentrations considerably lower than the RS. Discussion DPS or delayed centrifugation are viable pre‐analytical procedures for the accurate quantification of plasma NfL.https://doi.org/10.1002/dad2.12145dried blood spotsdried plasma spotsneurofilament lightpre‐analytical handling |
| spellingShingle | Joel Simrén Nicholas J. Ashton Kaj Blennow Henrik Zetterberg Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre‐analytical conditions Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring dried blood spots dried plasma spots neurofilament light pre‐analytical handling |
| title | Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre‐analytical conditions |
| title_full | Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre‐analytical conditions |
| title_fullStr | Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre‐analytical conditions |
| title_full_unstemmed | Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre‐analytical conditions |
| title_short | Blood neurofilament light in remote settings: Alternative protocols to support sample collection in challenging pre‐analytical conditions |
| title_sort | blood neurofilament light in remote settings alternative protocols to support sample collection in challenging pre analytical conditions |
| topic | dried blood spots dried plasma spots neurofilament light pre‐analytical handling |
| url | https://doi.org/10.1002/dad2.12145 |
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