Simplified LC-MS Method for Analysis of Sterols in Biological Samples
We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/18/4116 |
| _version_ | 1827706571662032896 |
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| author | Cene Skubic Irena Vovk Damjana Rozman Mitja Križman |
| author_facet | Cene Skubic Irena Vovk Damjana Rozman Mitja Križman |
| author_sort | Cene Skubic |
| collection | DOAJ |
| description | We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and dihydrolanosterol) from cultured human hepatocytes in a single chromatographic run using a pentafluorophenyl (PFP) stationary phase. The method also avails on a minimized sample preparation procedure in order to obtain a relatively high sample throughput. The method was validated on 10 sterol standards that were detected in a single chromatographic LC-MS run without derivatization. Our developed method can be used in research or clinical applications for disease-related detection of accumulated cholesterol intermediates. Disorders in the late part of cholesterol synthesis lead to severe malformation in human patients. The developed method enables a simple, sensitive, and fast quantification of sterols, without the need of extended knowledge of the LC-MS technique, and represents a new analytical tool in the rising field of cholesterolomics. |
| first_indexed | 2024-03-10T16:28:21Z |
| format | Article |
| id | doaj.art-244362940219495aaea1f7a42cf61340 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:28:21Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-244362940219495aaea1f7a42cf613402023-11-20T13:03:13ZengMDPI AGMolecules1420-30492020-09-012518411610.3390/molecules25184116Simplified LC-MS Method for Analysis of Sterols in Biological SamplesCene Skubic0Irena Vovk1Damjana Rozman2Mitja Križman3Center for Functional Genomics and Bio-Chips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Zaloška cesta 4, SI-1000 Ljubljana, SloveniaDepartment of Food Chemistry, National Institute of Chemistry, Ljubljana, Hajdrihova 19, SI-1000 Ljubljana, SloveniaCenter for Functional Genomics and Bio-Chips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Zaloška cesta 4, SI-1000 Ljubljana, SloveniaDepartment of Food Chemistry, National Institute of Chemistry, Ljubljana, Hajdrihova 19, SI-1000 Ljubljana, SloveniaWe developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and dihydrolanosterol) from cultured human hepatocytes in a single chromatographic run using a pentafluorophenyl (PFP) stationary phase. The method also avails on a minimized sample preparation procedure in order to obtain a relatively high sample throughput. The method was validated on 10 sterol standards that were detected in a single chromatographic LC-MS run without derivatization. Our developed method can be used in research or clinical applications for disease-related detection of accumulated cholesterol intermediates. Disorders in the late part of cholesterol synthesis lead to severe malformation in human patients. The developed method enables a simple, sensitive, and fast quantification of sterols, without the need of extended knowledge of the LC-MS technique, and represents a new analytical tool in the rising field of cholesterolomics.https://www.mdpi.com/1420-3049/25/18/4116cholesterolLC-MSsterol intermediatescholesterol synthesispentafluorophenylPFP |
| spellingShingle | Cene Skubic Irena Vovk Damjana Rozman Mitja Križman Simplified LC-MS Method for Analysis of Sterols in Biological Samples Molecules cholesterol LC-MS sterol intermediates cholesterol synthesis pentafluorophenyl PFP |
| title | Simplified LC-MS Method for Analysis of Sterols in Biological Samples |
| title_full | Simplified LC-MS Method for Analysis of Sterols in Biological Samples |
| title_fullStr | Simplified LC-MS Method for Analysis of Sterols in Biological Samples |
| title_full_unstemmed | Simplified LC-MS Method for Analysis of Sterols in Biological Samples |
| title_short | Simplified LC-MS Method for Analysis of Sterols in Biological Samples |
| title_sort | simplified lc ms method for analysis of sterols in biological samples |
| topic | cholesterol LC-MS sterol intermediates cholesterol synthesis pentafluorophenyl PFP |
| url | https://www.mdpi.com/1420-3049/25/18/4116 |
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