The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, Brazil
Streptococcus agalactiae isolates are more common among pregnant women, neonates and nonpregnant adults with underlying diseases compared to other demographic groups. In this study, we evaluate the genetic and phenotypic diversity in S. agalactiae strains from Rio de Janeiro (RJ) that were isolated...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800017&lng=en&tlng=en |
| _version_ | 1797757096349925376 |
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| author | Ana Beatriz de Almeida Corrêa Lígia Guedes da Silva Tatiana de Castro Abreu Pinto Ivi Cristina Menezes de Oliveira Flávio Gimenis Fernandes Natalia Silva da Costa Marcos Corrêa de Mattos Sergio Eduardo Longo Fracalanzza Leslie Claude Benchetrit |
| author_facet | Ana Beatriz de Almeida Corrêa Lígia Guedes da Silva Tatiana de Castro Abreu Pinto Ivi Cristina Menezes de Oliveira Flávio Gimenis Fernandes Natalia Silva da Costa Marcos Corrêa de Mattos Sergio Eduardo Longo Fracalanzza Leslie Claude Benchetrit |
| author_sort | Ana Beatriz de Almeida Corrêa |
| collection | DOAJ |
| description | Streptococcus agalactiae isolates are more common among pregnant women, neonates and nonpregnant adults with underlying diseases compared to other demographic groups. In this study, we evaluate the genetic and phenotypic diversity in S. agalactiae strains from Rio de Janeiro (RJ) that were isolated from asymptomatic carriers. We analysed these S. agalactiae strains using pulsed-field gel electrophoresis (PFGE), serotyping and antimicrobial susceptibility testing, as well as by determining the macrolide resistance phenotype, and detecting the presence of the ermA/B, mefA/E and lnuB genes. The serotypes Ia, II, III and V were the most prevalent serotypes observed. The 60 strains analysed were susceptible to penicillin, vancomycin and levofloxacin. Resistance to clindamycin, chloramphenicol, erythromycin, rifampin and tetracycline was observed. Among the erythromycin and/or clindamycin resistant strains, the ermA, ermB and mefA/E genes were detected and the constitutive macrolides, lincosamides and streptogramin B-type resistance was the most prevalent phenotype observed. The lnuB gene was not detected in any of the strains studied. We found 56 PFGE electrophoretic profiles and only 22 of them were allocated in polymorphism patterns. This work presents data on the genetic diversity and prevalent capsular serotypes among RJ isolates. Approximately 85% of these strains came from pregnant women; therefore, these data may be helpful in developing future prophylaxis and treatment strategies for neonatal syndromes in RJ. |
| first_indexed | 2024-03-12T18:11:03Z |
| format | Article |
| id | doaj.art-24457efbc6004321ac3458caa926abbe |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:11:03Z |
| publishDate | 2011-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-24457efbc6004321ac3458caa926abbe2023-08-02T09:15:32ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-12-0110681002100610.1590/S0074-02762011000800017S0074-02762011000800017The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, BrazilAna Beatriz de Almeida Corrêa0Lígia Guedes da Silva1Tatiana de Castro Abreu Pinto2Ivi Cristina Menezes de Oliveira3Flávio Gimenis Fernandes4Natalia Silva da Costa5Marcos Corrêa de Mattos6Sergio Eduardo Longo Fracalanzza7Leslie Claude Benchetrit8Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroStreptococcus agalactiae isolates are more common among pregnant women, neonates and nonpregnant adults with underlying diseases compared to other demographic groups. In this study, we evaluate the genetic and phenotypic diversity in S. agalactiae strains from Rio de Janeiro (RJ) that were isolated from asymptomatic carriers. We analysed these S. agalactiae strains using pulsed-field gel electrophoresis (PFGE), serotyping and antimicrobial susceptibility testing, as well as by determining the macrolide resistance phenotype, and detecting the presence of the ermA/B, mefA/E and lnuB genes. The serotypes Ia, II, III and V were the most prevalent serotypes observed. The 60 strains analysed were susceptible to penicillin, vancomycin and levofloxacin. Resistance to clindamycin, chloramphenicol, erythromycin, rifampin and tetracycline was observed. Among the erythromycin and/or clindamycin resistant strains, the ermA, ermB and mefA/E genes were detected and the constitutive macrolides, lincosamides and streptogramin B-type resistance was the most prevalent phenotype observed. The lnuB gene was not detected in any of the strains studied. We found 56 PFGE electrophoretic profiles and only 22 of them were allocated in polymorphism patterns. This work presents data on the genetic diversity and prevalent capsular serotypes among RJ isolates. Approximately 85% of these strains came from pregnant women; therefore, these data may be helpful in developing future prophylaxis and treatment strategies for neonatal syndromes in RJ.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800017&lng=en&tlng=enStreptococcus agalactiaepulsed-field gel electrophoresispolymerase chain reactionantimicrobial susceptibility |
| spellingShingle | Ana Beatriz de Almeida Corrêa Lígia Guedes da Silva Tatiana de Castro Abreu Pinto Ivi Cristina Menezes de Oliveira Flávio Gimenis Fernandes Natalia Silva da Costa Marcos Corrêa de Mattos Sergio Eduardo Longo Fracalanzza Leslie Claude Benchetrit The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, Brazil Memorias do Instituto Oswaldo Cruz Streptococcus agalactiae pulsed-field gel electrophoresis polymerase chain reaction antimicrobial susceptibility |
| title | The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, Brazil |
| title_full | The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, Brazil |
| title_fullStr | The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, Brazil |
| title_full_unstemmed | The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, Brazil |
| title_short | The genetic diversity and phenotypic characterisation of Streptococcus agalactiae isolates from Rio de Janeiro, Brazil |
| title_sort | genetic diversity and phenotypic characterisation of streptococcus agalactiae isolates from rio de janeiro brazil |
| topic | Streptococcus agalactiae pulsed-field gel electrophoresis polymerase chain reaction antimicrobial susceptibility |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800017&lng=en&tlng=en |
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