Lactic Acid Bacteria Isolated from Fresh Vegetable Products: Potential Probiotic and Postbiotic Characteristics Including Immunomodulatory Effects
The ability to perform effectively in the gastrointestinal tract (GIT) is one of the most significant criteria in the selection of potential probiotic bacteria. Thus, the present study aimed to investigate the potential probiotic characteristics of some selected lactic acid bacteria (LAB) isolated f...
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/389 |
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| author | Fatima Alameri Mohammad Tarique Tareq Osaili Riyad Obaid Abdelmoneim Abdalla Razan Masad Ashraf Al-Sbiei Maria Fernandez-Cabezudo Shao-Quan Liu Basel Al-Ramadi Mutamed Ayyash |
| author_facet | Fatima Alameri Mohammad Tarique Tareq Osaili Riyad Obaid Abdelmoneim Abdalla Razan Masad Ashraf Al-Sbiei Maria Fernandez-Cabezudo Shao-Quan Liu Basel Al-Ramadi Mutamed Ayyash |
| author_sort | Fatima Alameri |
| collection | DOAJ |
| description | The ability to perform effectively in the gastrointestinal tract (GIT) is one of the most significant criteria in the selection of potential probiotic bacteria. Thus, the present study aimed to investigate the potential probiotic characteristics of some selected lactic acid bacteria (LAB) isolated from vegetable products. Probiotic characteristics included tolerance to acid and bile, cholesterol-removing ability, bile salt hydrolysis, resistance against lysozyme and antibiotics, production of exopolysaccharides (EPS), antimicrobial and hemolytic activities, and cell surface characteristics (auto-aggregation, co-aggregation, and hydrophobicity). The survival rate of isolates after G120 ranged from 8.0 to 8.6 Log<sub>10</sub> CFU/mL. After the intestinal phase (IN-120), the bacterial count ranged from 7.3 to 8.5 Log<sub>10</sub> CFU/mL. The bile tolerance rates ranged from 17.8 to 51.1%, 33.6 to 63.9%, and 55.9 to 72.5% for cholic acid, oxgall, and taurocholic acid, respectively. Isolates F1, F8, F23, and F37 were able to reduce cholesterol (>30%) from the broth. The auto-aggregation average rate increased significantly after 24 h for all isolates, while two isolates showed the highest hydrophobicity values. Moreover, isolates had attachment capabilities comparable to those of HT-29 cells, with an average of 8.03 Log<sub>10</sub> CFU/mL after 2 h. All isolates were resistant to lysozyme and vancomycin, and 8 out of the 17 selected isolates displayed an ability to produce exopolysaccharides (EPS). Based on 16S rRNA sequencing, LAB isolates were identified as <i>Enterococcus faecium</i>, <i>E. durans</i>, <i>E. lactis</i>, and <i>Pediococcus acidilactici</i>. |
| first_indexed | 2024-03-09T21:23:26Z |
| format | Article |
| id | doaj.art-24478b4c02504df9807225f9595f2688 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:26Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-24478b4c02504df9807225f9595f26882023-11-23T21:15:23ZengMDPI AGMicroorganisms2076-26072022-02-0110238910.3390/microorganisms10020389Lactic Acid Bacteria Isolated from Fresh Vegetable Products: Potential Probiotic and Postbiotic Characteristics Including Immunomodulatory EffectsFatima Alameri0Mohammad Tarique1Tareq Osaili2Riyad Obaid3Abdelmoneim Abdalla4Razan Masad5Ashraf Al-Sbiei6Maria Fernandez-Cabezudo7Shao-Quan Liu8Basel Al-Ramadi9Mutamed Ayyash10Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU), Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU), Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Clinical Nutrition and Dietetics, College of Health Sciences, University of Sharjah, Sharjah P.O. Box 32223, United Arab EmiratesDepartment of Clinical Nutrition and Dietetics, College of Health Sciences, University of Sharjah, Sharjah P.O. Box 32223, United Arab EmiratesFood Science Department, College of Agriculture, South Valley University, Qena 83523, EgyptDepartment of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University (UAEU), Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Biochemistry & Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University (UAEU), Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Biochemistry & Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University (UAEU), Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Food Science and Technology, Faculty of Science, National University of Singapore, Singapore 117542, SingaporeDepartment of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University (UAEU), Al Ain P.O. Box 15551, United Arab EmiratesDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU), Al Ain P.O. Box 15551, United Arab EmiratesThe ability to perform effectively in the gastrointestinal tract (GIT) is one of the most significant criteria in the selection of potential probiotic bacteria. Thus, the present study aimed to investigate the potential probiotic characteristics of some selected lactic acid bacteria (LAB) isolated from vegetable products. Probiotic characteristics included tolerance to acid and bile, cholesterol-removing ability, bile salt hydrolysis, resistance against lysozyme and antibiotics, production of exopolysaccharides (EPS), antimicrobial and hemolytic activities, and cell surface characteristics (auto-aggregation, co-aggregation, and hydrophobicity). The survival rate of isolates after G120 ranged from 8.0 to 8.6 Log<sub>10</sub> CFU/mL. After the intestinal phase (IN-120), the bacterial count ranged from 7.3 to 8.5 Log<sub>10</sub> CFU/mL. The bile tolerance rates ranged from 17.8 to 51.1%, 33.6 to 63.9%, and 55.9 to 72.5% for cholic acid, oxgall, and taurocholic acid, respectively. Isolates F1, F8, F23, and F37 were able to reduce cholesterol (>30%) from the broth. The auto-aggregation average rate increased significantly after 24 h for all isolates, while two isolates showed the highest hydrophobicity values. Moreover, isolates had attachment capabilities comparable to those of HT-29 cells, with an average of 8.03 Log<sub>10</sub> CFU/mL after 2 h. All isolates were resistant to lysozyme and vancomycin, and 8 out of the 17 selected isolates displayed an ability to produce exopolysaccharides (EPS). Based on 16S rRNA sequencing, LAB isolates were identified as <i>Enterococcus faecium</i>, <i>E. durans</i>, <i>E. lactis</i>, and <i>Pediococcus acidilactici</i>.https://www.mdpi.com/2076-2607/10/2/389autoaggregationantimicrobialcholesterol-loweringimmunomodulation |
| spellingShingle | Fatima Alameri Mohammad Tarique Tareq Osaili Riyad Obaid Abdelmoneim Abdalla Razan Masad Ashraf Al-Sbiei Maria Fernandez-Cabezudo Shao-Quan Liu Basel Al-Ramadi Mutamed Ayyash Lactic Acid Bacteria Isolated from Fresh Vegetable Products: Potential Probiotic and Postbiotic Characteristics Including Immunomodulatory Effects Microorganisms autoaggregation antimicrobial cholesterol-lowering immunomodulation |
| title | Lactic Acid Bacteria Isolated from Fresh Vegetable Products: Potential Probiotic and Postbiotic Characteristics Including Immunomodulatory Effects |
| title_full | Lactic Acid Bacteria Isolated from Fresh Vegetable Products: Potential Probiotic and Postbiotic Characteristics Including Immunomodulatory Effects |
| title_fullStr | Lactic Acid Bacteria Isolated from Fresh Vegetable Products: Potential Probiotic and Postbiotic Characteristics Including Immunomodulatory Effects |
| title_full_unstemmed | Lactic Acid Bacteria Isolated from Fresh Vegetable Products: Potential Probiotic and Postbiotic Characteristics Including Immunomodulatory Effects |
| title_short | Lactic Acid Bacteria Isolated from Fresh Vegetable Products: Potential Probiotic and Postbiotic Characteristics Including Immunomodulatory Effects |
| title_sort | lactic acid bacteria isolated from fresh vegetable products potential probiotic and postbiotic characteristics including immunomodulatory effects |
| topic | autoaggregation antimicrobial cholesterol-lowering immunomodulation |
| url | https://www.mdpi.com/2076-2607/10/2/389 |
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