Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells
Objective To investigate the effect of actin binding protein ANLN on the proliferation, apoptosis, migration and invasion of gastric cancer cells in vitro. Methods RT-PCR and Western blot were used to detect the expression of ANLN in gastric cancer cell lines and gastric mucosal cell lines and verif...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2019-10-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2019.19.0338.htm |
| _version_ | 1818260448894517248 |
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| author | SUN Hui CHEN Feng LIU Dongyang SHI Ke PENG Qing |
| author_facet | SUN Hui CHEN Feng LIU Dongyang SHI Ke PENG Qing |
| author_sort | SUN Hui |
| collection | DOAJ |
| description | Objective To investigate the effect of actin binding protein ANLN on the proliferation, apoptosis, migration and invasion of gastric cancer cells in vitro. Methods RT-PCR and Western blot were used to detect the expression of ANLN in gastric cancer cell lines and gastric mucosal cell lines and verify the knockdown of ANLN gene by siRNA. The effect of silenced ANLN gene expression on the migration and invasion abilities of gastric cancer cells was detected by scratch test and Transwell assay. CCK-8 assay, cell fluorescence staining and flow cytometry were used to detect the effects of silent ANLN gene expression on the proliferation and apoptosis of gastric cancer cells. Results The expression of ANLN mRNA and protein in gastric cancer cell line SGC-7901/MGC-803 were significantly higher than those in gastric mucosa cell line GES-1 (P < 0.001). The transfection was successful, and the mRNA and protein expressions in the transfection group were statistically different from those in the control group (P < 0.05). Down-regulating ANLN expression significantly reduced the migration, invasion, proliferation and apoptosis of gastric cancer cells. Conclusion Down-regulating ANLN expression could significantly reduce the infiltration, migration, proliferation and apoptosis of gastric cancer cells. |
| first_indexed | 2024-12-12T18:31:30Z |
| format | Article |
| id | doaj.art-24479c8dbaee4264b87664849cc7f982 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-12T18:31:30Z |
| publishDate | 2019-10-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-24479c8dbaee4264b87664849cc7f9822022-12-22T00:15:55ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782019-10-01461088488910.3971/j.issn.1000-8578.2019.19.03388578.2019.19.0338Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer CellsSUN Hui0CHEN Feng1LIU Dongyang2SHI Ke3PENG Qing4College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaDepartment of General Surgery, Shanghai Sixth People's Hospital East Campus, Shanghai 201306, ChinaDepartment of General Surgery, Shanghai Sixth People's Hospital East Campus, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaDepartment of General Surgery, Shanghai Sixth People's Hospital East Campus Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201306, ChinaObjective To investigate the effect of actin binding protein ANLN on the proliferation, apoptosis, migration and invasion of gastric cancer cells in vitro. Methods RT-PCR and Western blot were used to detect the expression of ANLN in gastric cancer cell lines and gastric mucosal cell lines and verify the knockdown of ANLN gene by siRNA. The effect of silenced ANLN gene expression on the migration and invasion abilities of gastric cancer cells was detected by scratch test and Transwell assay. CCK-8 assay, cell fluorescence staining and flow cytometry were used to detect the effects of silent ANLN gene expression on the proliferation and apoptosis of gastric cancer cells. Results The expression of ANLN mRNA and protein in gastric cancer cell line SGC-7901/MGC-803 were significantly higher than those in gastric mucosa cell line GES-1 (P < 0.001). The transfection was successful, and the mRNA and protein expressions in the transfection group were statistically different from those in the control group (P < 0.05). Down-regulating ANLN expression significantly reduced the migration, invasion, proliferation and apoptosis of gastric cancer cells. Conclusion Down-regulating ANLN expression could significantly reduce the infiltration, migration, proliferation and apoptosis of gastric cancer cells.http://html.rhhz.net/ZLFZYJ/html/8578.2019.19.0338.htmanlngastric cancerapoptosismigrationproliferation |
| spellingShingle | SUN Hui CHEN Feng LIU Dongyang SHI Ke PENG Qing Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells Zhongliu Fangzhi Yanjiu anln gastric cancer apoptosis migration proliferation |
| title | Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells |
| title_full | Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells |
| title_fullStr | Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells |
| title_full_unstemmed | Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells |
| title_short | Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells |
| title_sort | effect of anln gene silencing on invasion migration proliferation and apoptosis of gastric cancer cells |
| topic | anln gastric cancer apoptosis migration proliferation |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2019.19.0338.htm |
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