Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by <i>Candida albicans</i> Resistant to Fluconazole
Background: It has been demonstrated that azole-resistant strains of <i>Candida albicans</i> have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts. For this reason, the present study evaluated the efficacy of aPDT, tog...
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MDPI AG
2019-09-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/12/3/140 |
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| author | Karem Janeth Rimachi Hidalgo Juliana Cabrini Carmello Cláudia Carolina Jordão Paula Aboud Barbugli Carlos Alberto de Sousa Costa Ewerton Garcia de Oliveira Mima Ana Claudia Pavarina |
| author_facet | Karem Janeth Rimachi Hidalgo Juliana Cabrini Carmello Cláudia Carolina Jordão Paula Aboud Barbugli Carlos Alberto de Sousa Costa Ewerton Garcia de Oliveira Mima Ana Claudia Pavarina |
| author_sort | Karem Janeth Rimachi Hidalgo |
| collection | DOAJ |
| description | Background: It has been demonstrated that azole-resistant strains of <i>Candida albicans</i> have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts. For this reason, the present study evaluated the efficacy of aPDT, together with nystatin (NYS), in the treatment of oral candidiasis in vivo. Methods: Mice were infected with fluconazole-resistant <i>C. albicans</i> (ATCC 96901). To perform the combined therapy, aPDT, mediated by Photodithazine (PDZ) and LED light, was used together with NYS. The efficacy of the treatments was evaluated by microbiological, macroscopic, histopathological and Confocal Scanning Laser Microscopy analyses of the lesions. The expression of p21 and p53, proteins associated with cell death, from the tongues of mice, was also performed. Results: The combined therapy reduced the fungal viability by around 2.6 log<sub>10</sub> and decreased the oral lesions and the inflammatory reaction. Additionally, it stimulated the production of p53 and p21. Conclusions: The combined therapy is a promising alternative treatment for oral candidiasis induced by <i>C. albicans</i> resistant to fluconazole. |
| first_indexed | 2024-12-14T03:14:51Z |
| format | Article |
| id | doaj.art-24488cc3f5ef47b5aaf61c4e373fd49b |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-12-14T03:14:51Z |
| publishDate | 2019-09-01 |
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| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-24488cc3f5ef47b5aaf61c4e373fd49b2022-12-21T23:19:10ZengMDPI AGPharmaceuticals1424-82472019-09-0112314010.3390/ph12030140ph12030140Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by <i>Candida albicans</i> Resistant to FluconazoleKarem Janeth Rimachi Hidalgo0Juliana Cabrini Carmello1Cláudia Carolina Jordão2Paula Aboud Barbugli3Carlos Alberto de Sousa Costa4Ewerton Garcia de Oliveira Mima5Ana Claudia Pavarina6Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, Rua Humaitá, 1680, Araraquara 14801-903, SP, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, Rua Humaitá, 1680, Araraquara 14801-903, SP, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, Rua Humaitá, 1680, Araraquara 14801-903, SP, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, Rua Humaitá, 1680, Araraquara 14801-903, SP, BrazilDepartment of Physiology and Pathology, School of Dentistry, São Paulo State University (Unesp), Araraquara, Rua Humaitá, 1680, Araraquara 14801-903, SP, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, Rua Humaitá, 1680, Araraquara 14801-903, SP, BrazilDepartment of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, Rua Humaitá, 1680, Araraquara 14801-903, SP, BrazilBackground: It has been demonstrated that azole-resistant strains of <i>Candida albicans</i> have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts. For this reason, the present study evaluated the efficacy of aPDT, together with nystatin (NYS), in the treatment of oral candidiasis in vivo. Methods: Mice were infected with fluconazole-resistant <i>C. albicans</i> (ATCC 96901). To perform the combined therapy, aPDT, mediated by Photodithazine (PDZ) and LED light, was used together with NYS. The efficacy of the treatments was evaluated by microbiological, macroscopic, histopathological and Confocal Scanning Laser Microscopy analyses of the lesions. The expression of p21 and p53, proteins associated with cell death, from the tongues of mice, was also performed. Results: The combined therapy reduced the fungal viability by around 2.6 log<sub>10</sub> and decreased the oral lesions and the inflammatory reaction. Additionally, it stimulated the production of p53 and p21. Conclusions: The combined therapy is a promising alternative treatment for oral candidiasis induced by <i>C. albicans</i> resistant to fluconazole.https://www.mdpi.com/1424-8247/12/3/140photodynamic therapy<i>Candida albicans</i>antifungals |
| spellingShingle | Karem Janeth Rimachi Hidalgo Juliana Cabrini Carmello Cláudia Carolina Jordão Paula Aboud Barbugli Carlos Alberto de Sousa Costa Ewerton Garcia de Oliveira Mima Ana Claudia Pavarina Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by <i>Candida albicans</i> Resistant to Fluconazole Pharmaceuticals photodynamic therapy <i>Candida albicans</i> antifungals |
| title | Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by <i>Candida albicans</i> Resistant to Fluconazole |
| title_full | Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by <i>Candida albicans</i> Resistant to Fluconazole |
| title_fullStr | Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by <i>Candida albicans</i> Resistant to Fluconazole |
| title_full_unstemmed | Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by <i>Candida albicans</i> Resistant to Fluconazole |
| title_short | Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by <i>Candida albicans</i> Resistant to Fluconazole |
| title_sort | antimicrobial photodynamic therapy in combination with nystatin in the treatment of experimental oral candidiasis induced by i candida albicans i resistant to fluconazole |
| topic | photodynamic therapy <i>Candida albicans</i> antifungals |
| url | https://www.mdpi.com/1424-8247/12/3/140 |
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