Morphological, Molecular, and Pathogenic Characterization of <i>Neofabraea alba</i>, a Postharvest Pathogen of Apple in Serbia
<i>Neofabraea</i> spp. is one of the major causal agents of the postharvest decay of apple fruit. Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/9/2015 |
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| author | Jelena Vukotić Vera Stojšin Nevena Nagl Mladen Petreš Jovana Hrustić Mila Grahovac Brankica Tanović |
| author_facet | Jelena Vukotić Vera Stojšin Nevena Nagl Mladen Petreš Jovana Hrustić Mila Grahovac Brankica Tanović |
| author_sort | Jelena Vukotić |
| collection | DOAJ |
| description | <i>Neofabraea</i> spp. is one of the major causal agents of the postharvest decay of apple fruit. Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with <i>Neofabraea</i> spp. Using polymerase chain reaction, phylogenetic analysis, and morphological characterization, <i>Neofabraea</i><i>alba</i> was identified as the causal agent of Bull’s eye rot. Twenty-five selected isolates were analyzed by DNA sequencing of the internal transcribed spacer and 16 S mitochondrial ribosomal RNA. The phylogenetic analyses provided information on the distance between <i>Neofabraea</i> spp., while merged phylogenetic tree was required to give insight and clarify the relationships between isolates of <i>Neofabraea</i><i>alba</i>. <i>N. alba</i> demonstrated pathogenicity on apple fruit as well as on branches, with significantly different aggressiveness among the isolates. Isolates were able to produce larger cankers and acervuli on apple branches with abundant sporulation All identified isolates were separated into two morphotypes and described based on their macromorphological characteristics on culture media. It was noticed that different culture media, pH, and temperature could affect macromorphological characteristics and development. Representative isolates were able to grow at a temperature range of 0 to 25 °C, above which only one isolate was able to grow on culture media. All isolates showed growth at a pH range of 2–10, with significant differences detected between isolates at different pH values. The type of cultivation medium significantly affected the size and shape of conidia, while the presence of the microconidia was recorded within isolates. The aim of this study was to identify the causal agents of Bull’s eye rot on stored apples in Serbia in the period 2017–2018 and characterize the obtained isolates based on pathogenic, morphological, physiological, and molecular criteria. |
| first_indexed | 2024-03-10T01:00:23Z |
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| id | doaj.art-244d7886cd4b48a1be113eb662cb5578 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T01:00:23Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-244d7886cd4b48a1be113eb662cb55782023-11-23T14:35:57ZengMDPI AGAgronomy2073-43952022-08-01129201510.3390/agronomy12092015Morphological, Molecular, and Pathogenic Characterization of <i>Neofabraea alba</i>, a Postharvest Pathogen of Apple in SerbiaJelena Vukotić0Vera Stojšin1Nevena Nagl2Mladen Petreš3Jovana Hrustić4Mila Grahovac5Brankica Tanović6Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaInstitute of Pesticides and Environmental Protection, Banatska 31 b, Zemun, 11080 Belgrade, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaInstitute of Pesticides and Environmental Protection, Banatska 31 b, Zemun, 11080 Belgrade, Serbia<i>Neofabraea</i> spp. is one of the major causal agents of the postharvest decay of apple fruit. Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with <i>Neofabraea</i> spp. Using polymerase chain reaction, phylogenetic analysis, and morphological characterization, <i>Neofabraea</i><i>alba</i> was identified as the causal agent of Bull’s eye rot. Twenty-five selected isolates were analyzed by DNA sequencing of the internal transcribed spacer and 16 S mitochondrial ribosomal RNA. The phylogenetic analyses provided information on the distance between <i>Neofabraea</i> spp., while merged phylogenetic tree was required to give insight and clarify the relationships between isolates of <i>Neofabraea</i><i>alba</i>. <i>N. alba</i> demonstrated pathogenicity on apple fruit as well as on branches, with significantly different aggressiveness among the isolates. Isolates were able to produce larger cankers and acervuli on apple branches with abundant sporulation All identified isolates were separated into two morphotypes and described based on their macromorphological characteristics on culture media. It was noticed that different culture media, pH, and temperature could affect macromorphological characteristics and development. Representative isolates were able to grow at a temperature range of 0 to 25 °C, above which only one isolate was able to grow on culture media. All isolates showed growth at a pH range of 2–10, with significant differences detected between isolates at different pH values. The type of cultivation medium significantly affected the size and shape of conidia, while the presence of the microconidia was recorded within isolates. The aim of this study was to identify the causal agents of Bull’s eye rot on stored apples in Serbia in the period 2017–2018 and characterize the obtained isolates based on pathogenic, morphological, physiological, and molecular criteria.https://www.mdpi.com/2073-4395/12/9/2015<i>Neofabraea</i> spp.apple postharvest pathogenBull’s eye rotidentification |
| spellingShingle | Jelena Vukotić Vera Stojšin Nevena Nagl Mladen Petreš Jovana Hrustić Mila Grahovac Brankica Tanović Morphological, Molecular, and Pathogenic Characterization of <i>Neofabraea alba</i>, a Postharvest Pathogen of Apple in Serbia Agronomy <i>Neofabraea</i> spp. apple postharvest pathogen Bull’s eye rot identification |
| title | Morphological, Molecular, and Pathogenic Characterization of <i>Neofabraea alba</i>, a Postharvest Pathogen of Apple in Serbia |
| title_full | Morphological, Molecular, and Pathogenic Characterization of <i>Neofabraea alba</i>, a Postharvest Pathogen of Apple in Serbia |
| title_fullStr | Morphological, Molecular, and Pathogenic Characterization of <i>Neofabraea alba</i>, a Postharvest Pathogen of Apple in Serbia |
| title_full_unstemmed | Morphological, Molecular, and Pathogenic Characterization of <i>Neofabraea alba</i>, a Postharvest Pathogen of Apple in Serbia |
| title_short | Morphological, Molecular, and Pathogenic Characterization of <i>Neofabraea alba</i>, a Postharvest Pathogen of Apple in Serbia |
| title_sort | morphological molecular and pathogenic characterization of i neofabraea alba i a postharvest pathogen of apple in serbia |
| topic | <i>Neofabraea</i> spp. apple postharvest pathogen Bull’s eye rot identification |
| url | https://www.mdpi.com/2073-4395/12/9/2015 |
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