Nephroprotective Effect of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak
This study was designed to assess the nephroprotective effects of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> aqueous extracts and carvedilol on hyperoxaluria-induced urolithiasis and to scrutinize the possible roles of NF-κB, p53, Bcl-2, Bax and Bak. Phytochemica...
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2218-273X/10/9/1317 |
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| author | Osama M. Ahmed Hossam Ebaid El-Shaymaa El-Nahass Mahmoud Ragab Ibrahim M. Alhazza |
| author_facet | Osama M. Ahmed Hossam Ebaid El-Shaymaa El-Nahass Mahmoud Ragab Ibrahim M. Alhazza |
| author_sort | Osama M. Ahmed |
| collection | DOAJ |
| description | This study was designed to assess the nephroprotective effects of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> aqueous extracts and carvedilol on hyperoxaluria-induced urolithiasis and to scrutinize the possible roles of NF-κB, p53, Bcl-2, Bax and Bak. Phytochemical screening and GC-MS analysis of mushrooms’ aqueous extracts were also performed and revealed the presence of multiple antioxidant and anti-inflammatory components. Hyperoxaluria was induced in Wistar rats through the addition of 0.75% (<i>v</i>/<i>v</i>) ethylene glycol in drinking water for nine weeks. The ethylene glycol-administered rats were orally treated with <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> aqueous extracts (100 mg/kg) and carvedilol (30 mg/kg) daily during the last seven weeks. The study showed that <i>Pleurotus ostreatus</i>, <i>Agaricus bisporus</i> and carvedilol all successfully inhibited ethylene glycol-induced histological perturbations and the elevation of serum creatinine, serum urea, serum and urinary uric acid, serum, urinary and kidney oxalate, urine specific gravity, kidney calcium, kidney NF-κB, NF-κB p65, NF-κB p50, p53, Bax and Bak expressions as well as serum TNF-α and IL-1β levels. Moreover, the treatment decreased the reduction in urinary creatinine, urinary urea, ratios of urinary creatinine to serum creatinine and urinary urea to serum urea, Fex Urea and Bcl-2 expression in kidney. In conclusion, although <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> extracts and carvedilol all significantly inhibited the progression of nephrolithiasis and showed nephroprotective effects against ethylene glycol-induced kidney dysfunction, <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> seemed to be more effective than carvedilol. Moreover, the nephroprotective effects may be mediated via affecting NF-κB activation, extrinsic apoptosis and intrinsic apoptosis pathways. |
| first_indexed | 2024-03-10T16:20:20Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T16:20:20Z |
| publishDate | 2020-09-01 |
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| series | Biomolecules |
| spelling | doaj.art-244df8fc118849e8aa4229203b393b9c2023-11-20T13:41:42ZengMDPI AGBiomolecules2218-273X2020-09-01109131710.3390/biom10091317Nephroprotective Effect of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and BakOsama M. Ahmed0Hossam Ebaid1El-Shaymaa El-Nahass2Mahmoud Ragab3Ibrahim M. Alhazza4Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef P.O. 62521, EgyptDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef P.O. 62521, EgyptSohag General Hospital, Sohag 42511, EgyptDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaThis study was designed to assess the nephroprotective effects of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> aqueous extracts and carvedilol on hyperoxaluria-induced urolithiasis and to scrutinize the possible roles of NF-κB, p53, Bcl-2, Bax and Bak. Phytochemical screening and GC-MS analysis of mushrooms’ aqueous extracts were also performed and revealed the presence of multiple antioxidant and anti-inflammatory components. Hyperoxaluria was induced in Wistar rats through the addition of 0.75% (<i>v</i>/<i>v</i>) ethylene glycol in drinking water for nine weeks. The ethylene glycol-administered rats were orally treated with <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> aqueous extracts (100 mg/kg) and carvedilol (30 mg/kg) daily during the last seven weeks. The study showed that <i>Pleurotus ostreatus</i>, <i>Agaricus bisporus</i> and carvedilol all successfully inhibited ethylene glycol-induced histological perturbations and the elevation of serum creatinine, serum urea, serum and urinary uric acid, serum, urinary and kidney oxalate, urine specific gravity, kidney calcium, kidney NF-κB, NF-κB p65, NF-κB p50, p53, Bax and Bak expressions as well as serum TNF-α and IL-1β levels. Moreover, the treatment decreased the reduction in urinary creatinine, urinary urea, ratios of urinary creatinine to serum creatinine and urinary urea to serum urea, Fex Urea and Bcl-2 expression in kidney. In conclusion, although <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> extracts and carvedilol all significantly inhibited the progression of nephrolithiasis and showed nephroprotective effects against ethylene glycol-induced kidney dysfunction, <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> seemed to be more effective than carvedilol. Moreover, the nephroprotective effects may be mediated via affecting NF-κB activation, extrinsic apoptosis and intrinsic apoptosis pathways.https://www.mdpi.com/2218-273X/10/9/1317<i>Pleurotus ostreatus</i><i>Agaricus bisporus</i>carvedilolhyperoxaluriaurolithiasisethylene glycol |
| spellingShingle | Osama M. Ahmed Hossam Ebaid El-Shaymaa El-Nahass Mahmoud Ragab Ibrahim M. Alhazza Nephroprotective Effect of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak Biomolecules <i>Pleurotus ostreatus</i> <i>Agaricus bisporus</i> carvedilol hyperoxaluria urolithiasis ethylene glycol |
| title | Nephroprotective Effect of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak |
| title_full | Nephroprotective Effect of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak |
| title_fullStr | Nephroprotective Effect of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak |
| title_full_unstemmed | Nephroprotective Effect of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak |
| title_short | Nephroprotective Effect of <i>Pleurotus ostreatus</i> and <i>Agaricus bisporus</i> Extracts and Carvedilol on Ethylene Glycol-Induced Urolithiasis: Roles of NF-κB, p53, Bcl-2, Bax and Bak |
| title_sort | nephroprotective effect of i pleurotus ostreatus i and i agaricus bisporus i extracts and carvedilol on ethylene glycol induced urolithiasis roles of nf κb p53 bcl 2 bax and bak |
| topic | <i>Pleurotus ostreatus</i> <i>Agaricus bisporus</i> carvedilol hyperoxaluria urolithiasis ethylene glycol |
| url | https://www.mdpi.com/2218-273X/10/9/1317 |
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