Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice
Abstract Nicotine use is a leading cause of preventable deaths worldwide, and most of those who attempt to quit will relapse. While electronic cigarettes and other electronic nicotine delivery systems (ENDS) were presented as a safer alternative to traditional cigarettes and promoted as devices to h...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-56766-z |
| _version_ | 1797247316892057600 |
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| author | Laura B. Murdaugh Cristina Miliano Irene Chen Christine L. Faunce Luis A. Natividad Ann M. Gregus Matthew W. Buczynski |
| author_facet | Laura B. Murdaugh Cristina Miliano Irene Chen Christine L. Faunce Luis A. Natividad Ann M. Gregus Matthew W. Buczynski |
| author_sort | Laura B. Murdaugh |
| collection | DOAJ |
| description | Abstract Nicotine use is a leading cause of preventable deaths worldwide, and most of those who attempt to quit will relapse. While electronic cigarettes and other electronic nicotine delivery systems (ENDS) were presented as a safer alternative to traditional cigarettes and promoted as devices to help traditional tobacco smokers reduce or quit smoking, they have instead contributed to increasing nicotine use among youths. Despite this, ENDS also represent a useful tool to create novel preclinical animal models of nicotine exposure that more accurately represent human nicotine use. In this study, we validated a chronic, intermittent, ENDS-based passive vapor exposure model in mice, and then measured changes in multiple behaviors related to nicotine abstinence. First, we performed a behavioral dose curve to investigate the effects of different nicotine inter-vape intervals on various measures including body weight, locomotor activity, and pain hypersensitivity. Next, we performed a pharmacokinetic study to measure plasma levels of nicotine and cotinine following chronic exposure for each inter-vape interval. Finally, we utilized a behavior test battery at a single dosing regimen that produces blood levels equivalent to human smokers in order to characterize the effects of chronic nicotine, vehicle, or passive airflow and identified nicotine-induced impairments in cognitive behavior. |
| first_indexed | 2024-04-24T19:56:46Z |
| format | Article |
| id | doaj.art-244ee4a574554aa3ae0ca3847aeec181 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T19:56:46Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-244ee4a574554aa3ae0ca3847aeec1812024-03-24T12:17:10ZengNature PortfolioScientific Reports2045-23222024-03-0114111310.1038/s41598-024-56766-zEffect of chronic vapor nicotine exposure on affective and cognitive behavior in male miceLaura B. Murdaugh0Cristina Miliano1Irene Chen2Christine L. Faunce3Luis A. Natividad4Ann M. Gregus5Matthew W. Buczynski6School of Neuroscience, Virginia Polytechnic Institute and State UniversitySchool of Neuroscience, Virginia Polytechnic Institute and State UniversitySchool of Neuroscience, Virginia Polytechnic Institute and State UniversitySchool of Neuroscience, Virginia Polytechnic Institute and State UniversityCollege of Pharmacy, Division of Pharmacology and Toxicology, University of Texas at AustinSchool of Neuroscience, Virginia Polytechnic Institute and State UniversitySchool of Neuroscience, Virginia Polytechnic Institute and State UniversityAbstract Nicotine use is a leading cause of preventable deaths worldwide, and most of those who attempt to quit will relapse. While electronic cigarettes and other electronic nicotine delivery systems (ENDS) were presented as a safer alternative to traditional cigarettes and promoted as devices to help traditional tobacco smokers reduce or quit smoking, they have instead contributed to increasing nicotine use among youths. Despite this, ENDS also represent a useful tool to create novel preclinical animal models of nicotine exposure that more accurately represent human nicotine use. In this study, we validated a chronic, intermittent, ENDS-based passive vapor exposure model in mice, and then measured changes in multiple behaviors related to nicotine abstinence. First, we performed a behavioral dose curve to investigate the effects of different nicotine inter-vape intervals on various measures including body weight, locomotor activity, and pain hypersensitivity. Next, we performed a pharmacokinetic study to measure plasma levels of nicotine and cotinine following chronic exposure for each inter-vape interval. Finally, we utilized a behavior test battery at a single dosing regimen that produces blood levels equivalent to human smokers in order to characterize the effects of chronic nicotine, vehicle, or passive airflow and identified nicotine-induced impairments in cognitive behavior.https://doi.org/10.1038/s41598-024-56766-zENDSe-cigaretteNicotineVapingCognitiveAddiction |
| spellingShingle | Laura B. Murdaugh Cristina Miliano Irene Chen Christine L. Faunce Luis A. Natividad Ann M. Gregus Matthew W. Buczynski Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice Scientific Reports ENDS e-cigarette Nicotine Vaping Cognitive Addiction |
| title | Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice |
| title_full | Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice |
| title_fullStr | Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice |
| title_full_unstemmed | Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice |
| title_short | Effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice |
| title_sort | effect of chronic vapor nicotine exposure on affective and cognitive behavior in male mice |
| topic | ENDS e-cigarette Nicotine Vaping Cognitive Addiction |
| url | https://doi.org/10.1038/s41598-024-56766-z |
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