Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of <i>Lentinus</i> (<i>Pleurotus</i>) <i>Sajor-caju</i>
The present study aimed to demonstrate <i>Lentinus</i> (formerly <i>Pleurotus</i>) <i>sajor-caju</i> (PSC) as a good source of pro-health substances. It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial prop...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/8/1089 |
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| author | Adrian Zając Mateusz Pięt Dawid Stefaniuk Michał Chojnacki Joanna Jakubowicz-Gil Roman Paduch Anna Matuszewska Magdalena Jaszek |
| author_facet | Adrian Zając Mateusz Pięt Dawid Stefaniuk Michał Chojnacki Joanna Jakubowicz-Gil Roman Paduch Anna Matuszewska Magdalena Jaszek |
| author_sort | Adrian Zając |
| collection | DOAJ |
| description | The present study aimed to demonstrate <i>Lentinus</i> (formerly <i>Pleurotus</i>) <i>sajor-caju</i> (PSC) as a good source of pro-health substances. It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial properties. Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition. Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro. Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control). Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts. This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods. |
| first_indexed | 2024-03-10T08:59:11Z |
| format | Article |
| id | doaj.art-2450db23774244ce86835891cd170a6e |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T08:59:11Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-2450db23774244ce86835891cd170a6e2023-11-22T06:54:37ZengMDPI AGBiomolecules2218-273X2021-07-01118108910.3390/biom11081089Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of <i>Lentinus</i> (<i>Pleurotus</i>) <i>Sajor-caju</i>Adrian Zając0Mateusz Pięt1Dawid Stefaniuk2Michał Chojnacki3Joanna Jakubowicz-Gil4Roman Paduch5Anna Matuszewska6Magdalena Jaszek7Department of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, PolandDepartment of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, PolandDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, PolandDepartment of Experimental Hematooncology, Medical University of Lublin, 20-031 Lublin, PolandDepartment of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, PolandDepartment of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, PolandDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, PolandDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, PolandThe present study aimed to demonstrate <i>Lentinus</i> (formerly <i>Pleurotus</i>) <i>sajor-caju</i> (PSC) as a good source of pro-health substances. It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial properties. Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition. Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro. Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control). Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts. This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods.https://www.mdpi.com/2218-273X/11/8/1089fungiarboreal medicinal mushrooms<i>Pleurotus sajor-caju</i><i>Lentinus sajor-caju</i>bioactive compoundscolorectal cancer |
| spellingShingle | Adrian Zając Mateusz Pięt Dawid Stefaniuk Michał Chojnacki Joanna Jakubowicz-Gil Roman Paduch Anna Matuszewska Magdalena Jaszek Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of <i>Lentinus</i> (<i>Pleurotus</i>) <i>Sajor-caju</i> Biomolecules fungi arboreal medicinal mushrooms <i>Pleurotus sajor-caju</i> <i>Lentinus sajor-caju</i> bioactive compounds colorectal cancer |
| title | Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of <i>Lentinus</i> (<i>Pleurotus</i>) <i>Sajor-caju</i> |
| title_full | Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of <i>Lentinus</i> (<i>Pleurotus</i>) <i>Sajor-caju</i> |
| title_fullStr | Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of <i>Lentinus</i> (<i>Pleurotus</i>) <i>Sajor-caju</i> |
| title_full_unstemmed | Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of <i>Lentinus</i> (<i>Pleurotus</i>) <i>Sajor-caju</i> |
| title_short | Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of <i>Lentinus</i> (<i>Pleurotus</i>) <i>Sajor-caju</i> |
| title_sort | pro health and anti cancer activity of fungal fractions isolated from milk supplemented cultures of i lentinus i i pleurotus i i sajor caju i |
| topic | fungi arboreal medicinal mushrooms <i>Pleurotus sajor-caju</i> <i>Lentinus sajor-caju</i> bioactive compounds colorectal cancer |
| url | https://www.mdpi.com/2218-273X/11/8/1089 |
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