Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid Nodules
In this study, galectin-3 was analyzed as a potential marker for preoperative detection of malignant thyroid lesions. Galectin-3 expression was analyzed by quantitative real-time reverse transcription polymerase chain reaction (RT -PCR) in preoperative thyroid fine-needle aspirates from 245 patie...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sestre Milosrdnice University hospital, Institute of Clinical Medical Research
2020-01-01
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| Series: | Acta Clinica Croatica |
| Subjects: | |
| Online Access: | https://hrcak.srce.hr/file/360823 |
| _version_ | 1818560047357100032 |
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| author | Ivan Šamija Neven Mateša Sanja Tadinac Tomislav Jukić |
| author_facet | Ivan Šamija Neven Mateša Sanja Tadinac Tomislav Jukić |
| author_sort | Ivan Šamija |
| collection | DOAJ |
| description | In this study, galectin-3 was analyzed as a potential marker for preoperative detection
of malignant thyroid lesions. Galectin-3 expression was analyzed by quantitative real-time reverse
transcription polymerase chain reaction (RT -PCR) in preoperative thyroid fine-needle aspirates from
245 patients with thyroid nodules. Out of 245 samples, 238 were adequate for analysis by RT -PCR.
Galectin-3 was positive in 34 (89.5%) of 38 papillary carcinomas, 3 (89.5%) of 4 follicular carcinomas,
17 (53.1%) of 32 follicular adenomas, 2 (33.3%) of 6 Hurthle cell adenoma, 11 (28.2%) of 39 Hashimoto
thyroiditis, and 69 (57.9%) of 119 nodular goiter samples. Galectin-3 showed specificity of
49.5%, sensitivity of 88.1%, positive predictive value of 27.2%, and negative predictive value of 95.1%
as a marker for detection of malignant thyroid nodules. Owing to the relatively low positive predictive
value due to the relatively high false positive rate, the clinical value of galectin-3 analyzed by quantitative
real-time RT -PCR as a marker for preoperative detection of malignant thyroid lesions is limited. |
| first_indexed | 2024-12-14T00:33:22Z |
| format | Article |
| id | doaj.art-245184dca2cf4258a48d5992ca84b089 |
| institution | Directory Open Access Journal |
| issn | 0353-9466 1333-9451 |
| language | English |
| last_indexed | 2024-12-14T00:33:22Z |
| publishDate | 2020-01-01 |
| publisher | Sestre Milosrdnice University hospital, Institute of Clinical Medical Research |
| record_format | Article |
| series | Acta Clinica Croatica |
| spelling | doaj.art-245184dca2cf4258a48d5992ca84b0892022-12-21T23:24:45ZengSestre Milosrdnice University hospital, Institute of Clinical Medical ResearchActa Clinica Croatica0353-94661333-94512020-01-0159.Supplement 12531Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid NodulesIvan Šamija0Neven Mateša1Sanja Tadinac2Tomislav Jukić3Department of Oncology and Nuclear Medicine, Sestre milosrdnice University Hospital Center, Zagreb, Croatia; Chair of Immunology, School of Dental Medicine, University of Zagreb, Zagreb, CroatiaDepartment of Oncology and Nuclear Medicine, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaDepartment of Oncology and Nuclear Medicine, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaDepartment of Oncology and Nuclear Medicine, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaIn this study, galectin-3 was analyzed as a potential marker for preoperative detection of malignant thyroid lesions. Galectin-3 expression was analyzed by quantitative real-time reverse transcription polymerase chain reaction (RT -PCR) in preoperative thyroid fine-needle aspirates from 245 patients with thyroid nodules. Out of 245 samples, 238 were adequate for analysis by RT -PCR. Galectin-3 was positive in 34 (89.5%) of 38 papillary carcinomas, 3 (89.5%) of 4 follicular carcinomas, 17 (53.1%) of 32 follicular adenomas, 2 (33.3%) of 6 Hurthle cell adenoma, 11 (28.2%) of 39 Hashimoto thyroiditis, and 69 (57.9%) of 119 nodular goiter samples. Galectin-3 showed specificity of 49.5%, sensitivity of 88.1%, positive predictive value of 27.2%, and negative predictive value of 95.1% as a marker for detection of malignant thyroid nodules. Owing to the relatively low positive predictive value due to the relatively high false positive rate, the clinical value of galectin-3 analyzed by quantitative real-time RT -PCR as a marker for preoperative detection of malignant thyroid lesions is limited.https://hrcak.srce.hr/file/360823thyroid nodulereverse transcriptase polymerase chain reactiongalectin-3thyroid neoplasmsfine-needle biopsy |
| spellingShingle | Ivan Šamija Neven Mateša Sanja Tadinac Tomislav Jukić Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid Nodules Acta Clinica Croatica thyroid nodule reverse transcriptase polymerase chain reaction galectin-3 thyroid neoplasms fine-needle biopsy |
| title | Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid Nodules |
| title_full | Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid Nodules |
| title_fullStr | Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid Nodules |
| title_full_unstemmed | Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid Nodules |
| title_short | Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid Nodules |
| title_sort | quantitative analysis of galectin 3 expression in benign and malignant thyroid nodules |
| topic | thyroid nodule reverse transcriptase polymerase chain reaction galectin-3 thyroid neoplasms fine-needle biopsy |
| url | https://hrcak.srce.hr/file/360823 |
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