Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing
Actinic keratosis (AK) arises on photo-damaged skin and is considered to be the precursor lesion of cutaneous squamous cell carcinoma (cSCC). Many findings support the involvement of β human papillomaviruses (HPVs) in cSCC, while very little is known on γ HPV types. The objective of this study was t...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Papillomavirus Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405852120300161 |
| _version_ | 1818342370552315904 |
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| author | Luisa Galati Rosario Nicola Brancaccio Alexis Robitaille Cyrille Cuenin Fabiola Luzi Gianna Fiorucci Maria Vincenza Chiantore Nadia Marascio Giovanni Matera Maria Carla Liberto Maria Gabriella Donà Paola Di Bonito Tarik Gheit Massimo Tommasino |
| author_facet | Luisa Galati Rosario Nicola Brancaccio Alexis Robitaille Cyrille Cuenin Fabiola Luzi Gianna Fiorucci Maria Vincenza Chiantore Nadia Marascio Giovanni Matera Maria Carla Liberto Maria Gabriella Donà Paola Di Bonito Tarik Gheit Massimo Tommasino |
| author_sort | Luisa Galati |
| collection | DOAJ |
| description | Actinic keratosis (AK) arises on photo-damaged skin and is considered to be the precursor lesion of cutaneous squamous cell carcinoma (cSCC). Many findings support the involvement of β human papillomaviruses (HPVs) in cSCC, while very little is known on γ HPV types. The objective of this study was to characterize the spectrum of PV types in healthy skin (HS) and AK samples of the same immunocompetent individuals using next generation sequencing (NGS). Viral DNA of 244 AK and 242 HS specimens were amplified by PCR using two different sets of primers (FAP59/64 and FAPM1). Purified amplicons were pooled and sequenced using NGS. The study resulted in the identification of a large number of known β and γ PV types. In addition, 27 putative novel β and 16 γ and 4 unclassified PVs were isolated. HPV types of species γ-1 (e.g. HPV4) appeared to be strongly enriched in AK versus HS. The NGS analysis revealed that a large spectrum of known and novel PVs is present in HS and AK. The evidence that species γ-1 HPV types appears to be enriched in AK in comparison to HS warrants further studies to evaluate their role in development of skin (pre)cancerous lesions. |
| first_indexed | 2024-12-13T16:13:37Z |
| format | Article |
| id | doaj.art-2454dbe2f0e74a5ba3a0900cc5b6e29e |
| institution | Directory Open Access Journal |
| issn | 2405-8521 |
| language | English |
| last_indexed | 2024-12-13T16:13:37Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Papillomavirus Research |
| spelling | doaj.art-2454dbe2f0e74a5ba3a0900cc5b6e29e2022-12-21T23:38:53ZengElsevierPapillomavirus Research2405-85212020-06-019Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencingLuisa Galati0Rosario Nicola Brancaccio1Alexis Robitaille2Cyrille Cuenin3Fabiola Luzi4Gianna Fiorucci5Maria Vincenza Chiantore6Nadia Marascio7Giovanni Matera8Maria Carla Liberto9Maria Gabriella Donà10Paola Di Bonito11Tarik Gheit12Massimo Tommasino13International Agency for Research on Cancer-World Health Organization, Lyon, France; ''Magna Graecia” University, Catanzaro, ItalyInternational Agency for Research on Cancer-World Health Organization, Lyon, FranceInternational Agency for Research on Cancer-World Health Organization, Lyon, FranceInternational Agency for Research on Cancer-World Health Organization, Lyon, FrancePlastic and Reconstructive Surgery, San Gallicano Dermatologic Institute IRCCS, Rome, ItalyDepartment of Infectious Diseases, EVOR Unit, Istituto Superiore di Sanità, Rome, Italy; Institute of Molecular Biology and Pathology, Consiglio Nazionale delle Ricerche, Rome, ItalyDepartment of Infectious Diseases, EVOR Unit, Istituto Superiore di Sanità, Rome, Italy''Magna Graecia” University, Catanzaro, Italy''Magna Graecia” University, Catanzaro, Italy''Magna Graecia” University, Catanzaro, ItalySTI/HIV Unit, San Gallicano Dermatologic Institute IRCCS, Rome, ItalyDepartment of Infectious Diseases, EVOR Unit, Istituto Superiore di Sanità, Rome, ItalyInternational Agency for Research on Cancer-World Health Organization, Lyon, FranceInternational Agency for Research on Cancer-World Health Organization, Lyon, France; Corresponding author. Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 cours Albert Thomas, 69372, Lyon Cedex 08, France.Actinic keratosis (AK) arises on photo-damaged skin and is considered to be the precursor lesion of cutaneous squamous cell carcinoma (cSCC). Many findings support the involvement of β human papillomaviruses (HPVs) in cSCC, while very little is known on γ HPV types. The objective of this study was to characterize the spectrum of PV types in healthy skin (HS) and AK samples of the same immunocompetent individuals using next generation sequencing (NGS). Viral DNA of 244 AK and 242 HS specimens were amplified by PCR using two different sets of primers (FAP59/64 and FAPM1). Purified amplicons were pooled and sequenced using NGS. The study resulted in the identification of a large number of known β and γ PV types. In addition, 27 putative novel β and 16 γ and 4 unclassified PVs were isolated. HPV types of species γ-1 (e.g. HPV4) appeared to be strongly enriched in AK versus HS. The NGS analysis revealed that a large spectrum of known and novel PVs is present in HS and AK. The evidence that species γ-1 HPV types appears to be enriched in AK in comparison to HS warrants further studies to evaluate their role in development of skin (pre)cancerous lesions.http://www.sciencedirect.com/science/article/pii/S2405852120300161HPVNGSActinic keratosisCutaneous squamous cell carcinoma (cSCC)Epidemiology |
| spellingShingle | Luisa Galati Rosario Nicola Brancaccio Alexis Robitaille Cyrille Cuenin Fabiola Luzi Gianna Fiorucci Maria Vincenza Chiantore Nadia Marascio Giovanni Matera Maria Carla Liberto Maria Gabriella Donà Paola Di Bonito Tarik Gheit Massimo Tommasino Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing Papillomavirus Research HPV NGS Actinic keratosis Cutaneous squamous cell carcinoma (cSCC) Epidemiology |
| title | Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing |
| title_full | Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing |
| title_fullStr | Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing |
| title_full_unstemmed | Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing |
| title_short | Detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing |
| title_sort | detection of human papillomaviruses in paired healthy skin and actinic keratosis by next generation sequencing |
| topic | HPV NGS Actinic keratosis Cutaneous squamous cell carcinoma (cSCC) Epidemiology |
| url | http://www.sciencedirect.com/science/article/pii/S2405852120300161 |
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