Association between omega-3/6 fatty acids and cholelithiasis: A mendelian randomization study
BackgroundOmega-3 and omega-6 may be protective factors for cholelithiasis. However, this relationship has not yet been demonstrated clearly. Therefore, we attempted to identify these causal relationships.Materials and methodsThe omega-3/6 fatty acid discovery dataset was obtained from UK Biobank an...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.964805/full |
| _version_ | 1818058119015563264 |
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| author | Qi Sun Qi Sun Ning Gao Weiliang Xia Weiliang Xia |
| author_facet | Qi Sun Qi Sun Ning Gao Weiliang Xia Weiliang Xia |
| author_sort | Qi Sun |
| collection | DOAJ |
| description | BackgroundOmega-3 and omega-6 may be protective factors for cholelithiasis. However, this relationship has not yet been demonstrated clearly. Therefore, we attempted to identify these causal relationships.Materials and methodsThe omega-3/6 fatty acid discovery dataset was obtained from UK Biobank and contained 114,999 individuals. The validation set was derived from an independent genome-wide association study (GWAS) and contained 13,544 individuals. The cholelithiasis dataset was derived from FinnGen and contained 19,023 cases and 195,144 controls. The inverse variance weighting (IVW) method was used as the main method of analysis in this study. Multiple methods of analysis were also used in the repeated methods, including the MR-Egger, weighted median, MR-pleiotropic residual sum (MR-PRESSO), outliers, and maximum likelihood methods. In addition, we used multiple sensitivity analyses to identify the potential pleiotropy.ResultIn the discovery stage, the results of the random effect IVW analysis showed that higher omega-3 levels were correlated inversely with the risk of cholelithiasis (β = –0.22, 95% CI [–0.32 to –0.12], P = 1.49 × 10–5). When the replication analysis was performed using another set of instrumental variables (IVs), the causal relationship between omega-3 fatty acids and cholelithiasis remained stable (β = –0.42, 95% CI [–0.66 to –0.18], P = 5.49 × 10–4), except for the results obtained using the MR-Egger method, which were not significant. The results of the IVW approach showed that each SD increase in omega-6 levels was associated negatively with the risk of cholelithiasis, both in the discovery (β = –0.21, 95% CI [–0.35 to –0.06], P = 4.37 × 10–3) and the validation phases (β = –0.21, 95% CI [–0.40 to –0.02], P = 3.44 × 10–2).ConclusionThe results of our MR study suggest that omega-3/6 is associated with cholelithiasis risk. Attention to the risk of cholelithiasis in individuals with low serum omega-3/6 levels is necessary. |
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| language | English |
| last_indexed | 2024-12-10T12:55:33Z |
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| spelling | doaj.art-24569c4c3fa44c4e96fe21a0f639813b2022-12-22T01:48:07ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.964805964805Association between omega-3/6 fatty acids and cholelithiasis: A mendelian randomization studyQi Sun0Qi Sun1Ning Gao2Weiliang Xia3Weiliang Xia4Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Cardiovascular Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaDivision of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaKey Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaBackgroundOmega-3 and omega-6 may be protective factors for cholelithiasis. However, this relationship has not yet been demonstrated clearly. Therefore, we attempted to identify these causal relationships.Materials and methodsThe omega-3/6 fatty acid discovery dataset was obtained from UK Biobank and contained 114,999 individuals. The validation set was derived from an independent genome-wide association study (GWAS) and contained 13,544 individuals. The cholelithiasis dataset was derived from FinnGen and contained 19,023 cases and 195,144 controls. The inverse variance weighting (IVW) method was used as the main method of analysis in this study. Multiple methods of analysis were also used in the repeated methods, including the MR-Egger, weighted median, MR-pleiotropic residual sum (MR-PRESSO), outliers, and maximum likelihood methods. In addition, we used multiple sensitivity analyses to identify the potential pleiotropy.ResultIn the discovery stage, the results of the random effect IVW analysis showed that higher omega-3 levels were correlated inversely with the risk of cholelithiasis (β = –0.22, 95% CI [–0.32 to –0.12], P = 1.49 × 10–5). When the replication analysis was performed using another set of instrumental variables (IVs), the causal relationship between omega-3 fatty acids and cholelithiasis remained stable (β = –0.42, 95% CI [–0.66 to –0.18], P = 5.49 × 10–4), except for the results obtained using the MR-Egger method, which were not significant. The results of the IVW approach showed that each SD increase in omega-6 levels was associated negatively with the risk of cholelithiasis, both in the discovery (β = –0.21, 95% CI [–0.35 to –0.06], P = 4.37 × 10–3) and the validation phases (β = –0.21, 95% CI [–0.40 to –0.02], P = 3.44 × 10–2).ConclusionThe results of our MR study suggest that omega-3/6 is associated with cholelithiasis risk. Attention to the risk of cholelithiasis in individuals with low serum omega-3/6 levels is necessary.https://www.frontiersin.org/articles/10.3389/fnut.2022.964805/fullcholelithiasispolyunsaturated acidsomega-3 (ω-3) and omega-6 (ω-6) fatty acidsmendelian randomisationcausal relationship |
| spellingShingle | Qi Sun Qi Sun Ning Gao Weiliang Xia Weiliang Xia Association between omega-3/6 fatty acids and cholelithiasis: A mendelian randomization study Frontiers in Nutrition cholelithiasis polyunsaturated acids omega-3 (ω-3) and omega-6 (ω-6) fatty acids mendelian randomisation causal relationship |
| title | Association between omega-3/6 fatty acids and cholelithiasis: A mendelian randomization study |
| title_full | Association between omega-3/6 fatty acids and cholelithiasis: A mendelian randomization study |
| title_fullStr | Association between omega-3/6 fatty acids and cholelithiasis: A mendelian randomization study |
| title_full_unstemmed | Association between omega-3/6 fatty acids and cholelithiasis: A mendelian randomization study |
| title_short | Association between omega-3/6 fatty acids and cholelithiasis: A mendelian randomization study |
| title_sort | association between omega 3 6 fatty acids and cholelithiasis a mendelian randomization study |
| topic | cholelithiasis polyunsaturated acids omega-3 (ω-3) and omega-6 (ω-6) fatty acids mendelian randomisation causal relationship |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.964805/full |
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