Discovery of Known and Novel Viral Genomes in Soybean Aphid by Deep Sequencing
The phytobiome includes not just cellular microorganisms, but also all viruses associated with plants: the virome. Plants and aphids exchange viruses regularly and efficiently; thus, the plant virome is tightly linked with the aphid virome. Yet, little is known about aphid viromes, particularly that...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2017-03-01
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| Series: | Phytobiomes Journal |
| Online Access: | https://doi.org/10.1094/PBIOMES-11-16-0013-R |
| _version_ | 1819172495612182528 |
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| author | Ying Feng Elizabeth N. Krueger Sijun Liu Karin Dorman Bryony C. Bonning W. Allen Miller |
| author_facet | Ying Feng Elizabeth N. Krueger Sijun Liu Karin Dorman Bryony C. Bonning W. Allen Miller |
| author_sort | Ying Feng |
| collection | DOAJ |
| description | The phytobiome includes not just cellular microorganisms, but also all viruses associated with plants: the virome. Plants and aphids exchange viruses regularly and efficiently; thus, the plant virome is tightly linked with the aphid virome. Yet, little is known about aphid viromes, particularly that of the soybean aphid (Aphis glycines), one of the most economically important pest insects of soybean. To sample the soybean aphid virome, and to seek new viruses as potential biological control agents, we employed next-generation sequencing. Genomes isolated from viruses in soybean aphids collected at four sites revealed many viruses, and six complete or nearly complete genomes were assembled. Most abundant were the picornavirus-like dicistroviruses Aphid lethal paralysis virus and Rhopalosiphum padi virus. We also sequenced the genome of a new dicistrovirus, Big Sioux River virus, fragments of which had been found previously in honey bee. Genome sequences that represent two entirely new virus families were obtained. These include an abundant tetravirus-like virus and a virus distantly related to cileviruses of plants and negeviruses of insects. Surprisingly, Cotton leafroll dwarf virus, a member of the genus Polerovirus, was found in soybean aphids from China, suggesting that the soybean aphid may be a vector of this virus. This virus had not been reported previously in China or in soybean. This study provides a peek into the rapidly expanding, largely unexplored world of insect viromes that will provide valuable knowledge for future understanding of plant−virus−vector interactions. |
| first_indexed | 2024-12-22T20:08:06Z |
| format | Article |
| id | doaj.art-24573008a5e74afcb861eeb8333252ed |
| institution | Directory Open Access Journal |
| issn | 2471-2906 |
| language | English |
| last_indexed | 2024-12-22T20:08:06Z |
| publishDate | 2017-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Phytobiomes Journal |
| spelling | doaj.art-24573008a5e74afcb861eeb8333252ed2022-12-21T18:14:06ZengThe American Phytopathological SocietyPhytobiomes Journal2471-29062017-03-0111364510.1094/PBIOMES-11-16-0013-RDiscovery of Known and Novel Viral Genomes in Soybean Aphid by Deep SequencingYing FengElizabeth N. KruegerSijun LiuKarin DormanBryony C. BonningW. Allen MillerThe phytobiome includes not just cellular microorganisms, but also all viruses associated with plants: the virome. Plants and aphids exchange viruses regularly and efficiently; thus, the plant virome is tightly linked with the aphid virome. Yet, little is known about aphid viromes, particularly that of the soybean aphid (Aphis glycines), one of the most economically important pest insects of soybean. To sample the soybean aphid virome, and to seek new viruses as potential biological control agents, we employed next-generation sequencing. Genomes isolated from viruses in soybean aphids collected at four sites revealed many viruses, and six complete or nearly complete genomes were assembled. Most abundant were the picornavirus-like dicistroviruses Aphid lethal paralysis virus and Rhopalosiphum padi virus. We also sequenced the genome of a new dicistrovirus, Big Sioux River virus, fragments of which had been found previously in honey bee. Genome sequences that represent two entirely new virus families were obtained. These include an abundant tetravirus-like virus and a virus distantly related to cileviruses of plants and negeviruses of insects. Surprisingly, Cotton leafroll dwarf virus, a member of the genus Polerovirus, was found in soybean aphids from China, suggesting that the soybean aphid may be a vector of this virus. This virus had not been reported previously in China or in soybean. This study provides a peek into the rapidly expanding, largely unexplored world of insect viromes that will provide valuable knowledge for future understanding of plant−virus−vector interactions.https://doi.org/10.1094/PBIOMES-11-16-0013-R |
| spellingShingle | Ying Feng Elizabeth N. Krueger Sijun Liu Karin Dorman Bryony C. Bonning W. Allen Miller Discovery of Known and Novel Viral Genomes in Soybean Aphid by Deep Sequencing Phytobiomes Journal |
| title | Discovery of Known and Novel Viral Genomes in Soybean Aphid by Deep Sequencing |
| title_full | Discovery of Known and Novel Viral Genomes in Soybean Aphid by Deep Sequencing |
| title_fullStr | Discovery of Known and Novel Viral Genomes in Soybean Aphid by Deep Sequencing |
| title_full_unstemmed | Discovery of Known and Novel Viral Genomes in Soybean Aphid by Deep Sequencing |
| title_short | Discovery of Known and Novel Viral Genomes in Soybean Aphid by Deep Sequencing |
| title_sort | discovery of known and novel viral genomes in soybean aphid by deep sequencing |
| url | https://doi.org/10.1094/PBIOMES-11-16-0013-R |
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