Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells
Cytoskeletal remodeling in circulating tumor cells (CTCs) facilitates metastatic spread. Previous oncology studies examine sustained aberrant calcium (Ca<sup>2+</sup>) signaling and cytoskeletal remodeling scrutinizing long-term phenotypes such as tumorigenesis and metastasis. The signif...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/3/884 |
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| author | Katarina T. Chang Keyata N. Thompson Stephen J. P. Pratt Julia A. Ju Rachel M. Lee Trevor J. Mathias Makenzy L. Mull David A. Annis Eleanor C. Ory Megan B. Stemberger Michele I. Vitolo Stuart S. Martin |
| author_facet | Katarina T. Chang Keyata N. Thompson Stephen J. P. Pratt Julia A. Ju Rachel M. Lee Trevor J. Mathias Makenzy L. Mull David A. Annis Eleanor C. Ory Megan B. Stemberger Michele I. Vitolo Stuart S. Martin |
| author_sort | Katarina T. Chang |
| collection | DOAJ |
| description | Cytoskeletal remodeling in circulating tumor cells (CTCs) facilitates metastatic spread. Previous oncology studies examine sustained aberrant calcium (Ca<sup>2+</sup>) signaling and cytoskeletal remodeling scrutinizing long-term phenotypes such as tumorigenesis and metastasis. The significance of acute Ca<sup>2+</sup> signaling in tumor cells that occur within seconds to minutes is overlooked. This study investigates rapid cytoplasmic Ca<sup>2+</sup> elevation in suspended cells on actin and tubulin cytoskeletal rearrangements and the metastatic microtentacle (McTN) phenotype. The compounds Ionomycin and Thapsigargin acutely increase cytoplasmic Ca<sup>2+</sup>, suppressing McTNs in the metastatic breast cancer cell lines MDA-MB-231 and MDA-MB-436. Functional decreases in McTN-mediated reattachment and cell clustering during the first 24 h of treatment are not attributed to cytotoxicity. Rapid cytoplasmic Ca<sup>2+</sup> elevation was correlated to Ca<sup>2+</sup>-induced actin cortex contraction and rearrangement via myosin light chain 2 and cofilin activity, while the inhibition of actin polymerization with Latrunculin A reversed Ca<sup>2+</sup>-mediated McTN suppression. Preclinical and phase 1 and 2 clinical trial data have established Thapsigargin derivatives as cytotoxic anticancer agents. The results from this study suggest an alternative molecular mechanism by which these compounds act, and proof-of-principle Ca<sup>2+</sup>-modulating compounds can rapidly induce morphological changes in free-floating tumor cells to reduce metastatic phenotypes. |
| first_indexed | 2024-03-11T09:49:51Z |
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| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T09:49:51Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-246052652eec4a51a1c2f6d1aae3e91e2023-11-16T16:18:38ZengMDPI AGCancers2072-66942023-01-0115388410.3390/cancers15030884Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor CellsKatarina T. Chang0Keyata N. Thompson1Stephen J. P. Pratt2Julia A. Ju3Rachel M. Lee4Trevor J. Mathias5Makenzy L. Mull6David A. Annis7Eleanor C. Ory8Megan B. Stemberger9Michele I. Vitolo10Stuart S. Martin11Graduate Program in Molecular Medicine, University of Maryland School of Medicine, 800 W. Baltimore St., Baltimore, MD 21201, USAMarlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USAMarlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USAGraduate Program in Molecular Medicine, University of Maryland School of Medicine, 800 W. Baltimore St., Baltimore, MD 21201, USAMarlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USAGraduate Program in Molecular Medicine, University of Maryland School of Medicine, 800 W. Baltimore St., Baltimore, MD 21201, USAGraduate Program in Molecular Medicine, University of Maryland School of Medicine, 800 W. Baltimore St., Baltimore, MD 21201, USAMarlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USAMarlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USAMarlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USAMarlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USAMarlene and Stewart Greenebaum NCI Comprehensive Cancer Center, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USACytoskeletal remodeling in circulating tumor cells (CTCs) facilitates metastatic spread. Previous oncology studies examine sustained aberrant calcium (Ca<sup>2+</sup>) signaling and cytoskeletal remodeling scrutinizing long-term phenotypes such as tumorigenesis and metastasis. The significance of acute Ca<sup>2+</sup> signaling in tumor cells that occur within seconds to minutes is overlooked. This study investigates rapid cytoplasmic Ca<sup>2+</sup> elevation in suspended cells on actin and tubulin cytoskeletal rearrangements and the metastatic microtentacle (McTN) phenotype. The compounds Ionomycin and Thapsigargin acutely increase cytoplasmic Ca<sup>2+</sup>, suppressing McTNs in the metastatic breast cancer cell lines MDA-MB-231 and MDA-MB-436. Functional decreases in McTN-mediated reattachment and cell clustering during the first 24 h of treatment are not attributed to cytotoxicity. Rapid cytoplasmic Ca<sup>2+</sup> elevation was correlated to Ca<sup>2+</sup>-induced actin cortex contraction and rearrangement via myosin light chain 2 and cofilin activity, while the inhibition of actin polymerization with Latrunculin A reversed Ca<sup>2+</sup>-mediated McTN suppression. Preclinical and phase 1 and 2 clinical trial data have established Thapsigargin derivatives as cytotoxic anticancer agents. The results from this study suggest an alternative molecular mechanism by which these compounds act, and proof-of-principle Ca<sup>2+</sup>-modulating compounds can rapidly induce morphological changes in free-floating tumor cells to reduce metastatic phenotypes.https://www.mdpi.com/2072-6694/15/3/884microtentacle (McTN)Ionomycin (Iono)Thapsigargin (Tg)calcium (Ca<sup>2+</sup>)breast cancermetastasis |
| spellingShingle | Katarina T. Chang Keyata N. Thompson Stephen J. P. Pratt Julia A. Ju Rachel M. Lee Trevor J. Mathias Makenzy L. Mull David A. Annis Eleanor C. Ory Megan B. Stemberger Michele I. Vitolo Stuart S. Martin Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells Cancers microtentacle (McTN) Ionomycin (Iono) Thapsigargin (Tg) calcium (Ca<sup>2+</sup>) breast cancer metastasis |
| title | Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells |
| title_full | Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells |
| title_fullStr | Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells |
| title_full_unstemmed | Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells |
| title_short | Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells |
| title_sort | elevation of cytoplasmic calcium suppresses microtentacle formation and function in breast tumor cells |
| topic | microtentacle (McTN) Ionomycin (Iono) Thapsigargin (Tg) calcium (Ca<sup>2+</sup>) breast cancer metastasis |
| url | https://www.mdpi.com/2072-6694/15/3/884 |
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