Transcriptome analysis of genes involved in flower and leaf color of Oncidium by RNA-seq
Oncidium, an important tropical orchid, has high ornamental value due to its specific color and occupies a significant market position for the worldwide flower. Transcriptome analysis of flower and leaf color formation provides new sources for producing novel Oncidium hybridum cultivars. We sequenc...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2023-10-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4831 |
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| author | Ma-Yin Wang Yu Ding Ye Zhang Lu Sun Xi-Qiang Song Dai-Cheng Hao Wei-Shi Li Min-Qiang Tang Peng Ling Shang-Qian Xie |
| author_facet | Ma-Yin Wang Yu Ding Ye Zhang Lu Sun Xi-Qiang Song Dai-Cheng Hao Wei-Shi Li Min-Qiang Tang Peng Ling Shang-Qian Xie |
| author_sort | Ma-Yin Wang |
| collection | DOAJ |
| description |
Oncidium, an important tropical orchid, has high ornamental value due to its specific color and occupies a significant market position for the worldwide flower. Transcriptome analysis of flower and leaf color formation provides new sources for producing novel Oncidium hybridum cultivars. We sequenced 12 samples of flowers (yellow and white) and leaves (striped and regular) of O. hybridum and assembled 381,136 and 453,566 unigene sequences from RNA-seq data, respectively. Among unigenes, 662 and 1,324 differentially expressed genes were identified in flower and leaf samples, respectively. Gene ontology and pathway enrichment showed that secondary metabolite biosynthetic pathways were responsible for flower and leaf color formation. It was determined that UGT75C1, E2.4.1.115, CCD7, E2.1.1.76, and CCoAOMT are involved in regulating flower color, and UGT75C1, LHCB, UGT, RP-L18Ae, and ABCB1 play crucial roles in regulating leaf color. Kyoto Encyclopedia of Genes and Genomes analysis revealed that UGT75C1 was significantly enriched in the anthocyanin biosynthetic pathway, showing effects on flower and leaf colors. This study was the first detailed analysis of the molecular mechanisms of O. hybridum flower and leaf colors, and the results advanced the understanding of the genetic basis of flower and leaf colors; they also provided additional support for improving commercial value and producing novel cultivars of O. hybridum.
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| first_indexed | 2024-03-09T00:13:59Z |
| format | Article |
| id | doaj.art-2460cfea507f40e1be49faf6e8aadac4 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:13:59Z |
| publishDate | 2023-10-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
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| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-2460cfea507f40e1be49faf6e8aadac42023-12-12T09:39:42ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052023-10-0122510.24326/asphc.2023.4831Transcriptome analysis of genes involved in flower and leaf color of Oncidium by RNA-seqMa-Yin Wang0Yu Ding1Ye Zhang2Lu Sun3Xi-Qiang Song4Dai-Cheng Hao5Wei-Shi Li6Min-Qiang Tang7Peng Ling8Shang-Qian Xie9Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, College of Horticulture, Hainan University, Haikou 570228, ChinaKey Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, College of Horticulture, Hainan University, Haikou 570228, ChinaKey Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, College of Horticulture, Hainan University, Haikou 570228, ChinaKey Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, College of Horticulture, Hainan University, Haikou 570228, ChinaKey Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, College of Horticulture, Hainan University, Haikou 570228, ChinaHainan Boda Orchid Technology Co. Ltd, Haikou 570311, ChinaHainan Boda Orchid Technology Co. Ltd, Haikou 570311, ChinaKey Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, College of Horticulture, Hainan University, Haikou 570228, ChinaKey Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, College of Horticulture, Hainan University, Haikou 570228, ChinaKey Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, College of Horticulture, Hainan University, Haikou 570228, China Oncidium, an important tropical orchid, has high ornamental value due to its specific color and occupies a significant market position for the worldwide flower. Transcriptome analysis of flower and leaf color formation provides new sources for producing novel Oncidium hybridum cultivars. We sequenced 12 samples of flowers (yellow and white) and leaves (striped and regular) of O. hybridum and assembled 381,136 and 453,566 unigene sequences from RNA-seq data, respectively. Among unigenes, 662 and 1,324 differentially expressed genes were identified in flower and leaf samples, respectively. Gene ontology and pathway enrichment showed that secondary metabolite biosynthetic pathways were responsible for flower and leaf color formation. It was determined that UGT75C1, E2.4.1.115, CCD7, E2.1.1.76, and CCoAOMT are involved in regulating flower color, and UGT75C1, LHCB, UGT, RP-L18Ae, and ABCB1 play crucial roles in regulating leaf color. Kyoto Encyclopedia of Genes and Genomes analysis revealed that UGT75C1 was significantly enriched in the anthocyanin biosynthetic pathway, showing effects on flower and leaf colors. This study was the first detailed analysis of the molecular mechanisms of O. hybridum flower and leaf colors, and the results advanced the understanding of the genetic basis of flower and leaf colors; they also provided additional support for improving commercial value and producing novel cultivars of O. hybridum. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4831Oncidium hybridumRNA-seqdifferential analysisdifferentially expressed geneskey genes |
| spellingShingle | Ma-Yin Wang Yu Ding Ye Zhang Lu Sun Xi-Qiang Song Dai-Cheng Hao Wei-Shi Li Min-Qiang Tang Peng Ling Shang-Qian Xie Transcriptome analysis of genes involved in flower and leaf color of Oncidium by RNA-seq Acta Scientiarum Polonorum: Hortorum Cultus Oncidium hybridum RNA-seq differential analysis differentially expressed genes key genes |
| title | Transcriptome analysis of genes involved in flower and leaf color of Oncidium by RNA-seq |
| title_full | Transcriptome analysis of genes involved in flower and leaf color of Oncidium by RNA-seq |
| title_fullStr | Transcriptome analysis of genes involved in flower and leaf color of Oncidium by RNA-seq |
| title_full_unstemmed | Transcriptome analysis of genes involved in flower and leaf color of Oncidium by RNA-seq |
| title_short | Transcriptome analysis of genes involved in flower and leaf color of Oncidium by RNA-seq |
| title_sort | transcriptome analysis of genes involved in flower and leaf color of oncidium by rna seq |
| topic | Oncidium hybridum RNA-seq differential analysis differentially expressed genes key genes |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4831 |
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