An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection
A sensitive, precise and selective ultra-high performance liquid chromatography method coupled with triple-quadrupole mass spectrometry was developed and validated for the determination of trace amounts of sinomenine (ng/mL) in minute volumes of human plasma. Fifty microliter plasma samples were p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/4/6454 |
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| author | Tingbo Chen Zheng Xiang Gengting Dong Pei Luo Ping Qiu Shenzhi Wang Baoming Huang Yingyi Wen Feichi Wu Liang Liu Hua Zhou |
| author_facet | Tingbo Chen Zheng Xiang Gengting Dong Pei Luo Ping Qiu Shenzhi Wang Baoming Huang Yingyi Wen Feichi Wu Liang Liu Hua Zhou |
| author_sort | Tingbo Chen |
| collection | DOAJ |
| description | A sensitive, precise and selective ultra-high performance liquid chromatography method coupled with triple-quadrupole mass spectrometry was developed and validated for the determination of trace amounts of sinomenine (ng/mL) in minute volumes of human plasma. Fifty microliter plasma samples were precipitated using methanol to extract sinomenine. Separation was carried out on a C18 column with a water and acetonitrile mobile phase gradient with formic acid as an additive. The mass spectrometry data were obtained in the positive ion mode, and the transition of multiple reactions was monitored at m/z 330.2→181.0 for sinomenine quantification. The working assay range for sinomenine was linear from 0.1173 to 15.02 ng/mL with the lower limit of quantification of 0.1173 ng/mL. The precision and accuracy of the method was less than 15% in intra-day and inter-day experiments with a matrix effect of less than 6.5%. After validation, the quantitative method was applied to analyze sinomenine levels in human plasma after transdermal delivery of the Zhengqing Fengtongning Injection. The results showed that some samples contained sinomenine within the concentration range 0.4131–4.407 ng/mL. |
| first_indexed | 2024-12-20T13:01:13Z |
| format | Article |
| id | doaj.art-24610bd9cad54decb4000e3288c29846 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T13:01:13Z |
| publishDate | 2015-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-24610bd9cad54decb4000e3288c298462022-12-21T19:39:54ZengMDPI AGMolecules1420-30492015-04-012046454646510.3390/molecules20046454molecules20046454An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning InjectionTingbo Chen0Zheng Xiang1Gengting Dong2Pei Luo3Ping Qiu4Shenzhi Wang5Baoming Huang6Yingyi Wen7Feichi Wu8Liang Liu9Hua Zhou10State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, ChinaHunan Zhengqing Pharmaceutical Group Limited, Huaihua 418005, ChinaThe First Hospital of Hunan University of Chinese Medicine, Changsha 410007, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, ChinaHunan Zhengqing Pharmaceutical Group Limited, Huaihua 418005, ChinaHunan Zhengqing Pharmaceutical Group Limited, Huaihua 418005, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, ChinaA sensitive, precise and selective ultra-high performance liquid chromatography method coupled with triple-quadrupole mass spectrometry was developed and validated for the determination of trace amounts of sinomenine (ng/mL) in minute volumes of human plasma. Fifty microliter plasma samples were precipitated using methanol to extract sinomenine. Separation was carried out on a C18 column with a water and acetonitrile mobile phase gradient with formic acid as an additive. The mass spectrometry data were obtained in the positive ion mode, and the transition of multiple reactions was monitored at m/z 330.2→181.0 for sinomenine quantification. The working assay range for sinomenine was linear from 0.1173 to 15.02 ng/mL with the lower limit of quantification of 0.1173 ng/mL. The precision and accuracy of the method was less than 15% in intra-day and inter-day experiments with a matrix effect of less than 6.5%. After validation, the quantitative method was applied to analyze sinomenine levels in human plasma after transdermal delivery of the Zhengqing Fengtongning Injection. The results showed that some samples contained sinomenine within the concentration range 0.4131–4.407 ng/mL.http://www.mdpi.com/1420-3049/20/4/6454sinomenineZhengqing Fengtongning Injectiontransdermal deliveryhuman plasmaconcentrationliquid chromatography-tandem mass spectrometry |
| spellingShingle | Tingbo Chen Zheng Xiang Gengting Dong Pei Luo Ping Qiu Shenzhi Wang Baoming Huang Yingyi Wen Feichi Wu Liang Liu Hua Zhou An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection Molecules sinomenine Zhengqing Fengtongning Injection transdermal delivery human plasma concentration liquid chromatography-tandem mass spectrometry |
| title | An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection |
| title_full | An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection |
| title_fullStr | An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection |
| title_full_unstemmed | An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection |
| title_short | An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection |
| title_sort | ultra high performance liquid chromatographic tandem mass spectrometric method for the determination of sinomenine in human plasma after transdermal delivery of the zhengqing fengtongning injection |
| topic | sinomenine Zhengqing Fengtongning Injection transdermal delivery human plasma concentration liquid chromatography-tandem mass spectrometry |
| url | http://www.mdpi.com/1420-3049/20/4/6454 |
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