Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources
Abstract Background Mesenchymal stem cells (MSCs) are undifferentiated cells that can give rise to a mesoderm lineage. Adipose-derived MSCs are an easy and accessible source for MSCs isolation, although each source of MSC has its own advantages and disadvantages. Our study identifies a promising sou...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-021-03100-8 |
| _version_ | 1819096186349420544 |
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| author | Usman Rashid Arfan Yousaf Muhammad Yaqoob Evelyn Saba Muhammad Moaeen-ud-Din Shahid Waseem Sandra K. Becker Gerhard Sponder Jörg R. Aschenbach Mansur Abdullah Sandhu |
| author_facet | Usman Rashid Arfan Yousaf Muhammad Yaqoob Evelyn Saba Muhammad Moaeen-ud-Din Shahid Waseem Sandra K. Becker Gerhard Sponder Jörg R. Aschenbach Mansur Abdullah Sandhu |
| author_sort | Usman Rashid |
| collection | DOAJ |
| description | Abstract Background Mesenchymal stem cells (MSCs) are undifferentiated cells that can give rise to a mesoderm lineage. Adipose-derived MSCs are an easy and accessible source for MSCs isolation, although each source of MSC has its own advantages and disadvantages. Our study identifies a promising source for the isolation and differentiation of canines MSCs. For this purpose, adipose tissue from inguinal subcutaneous (SC), perirenal (PR), omental (OM), and infrapatellar fat pad (IPFP) was isolated and processed for MSCs isolation. In the third passage, MSCs proliferation/metabolism, surface markers expression, in vitro differentiation potential and quantitative reverse transcription PCR (CD73, CD90, CD105, PPARγ, FabP4, FAS, SP7, Osteopontin, and Osteocalcin) were evaluated. Results Our results showed that MSCs derived from IPFP have a higher proliferation rate, while OM-derived MSCs have higher cell metabolism. In addition, MSCs from all adipose tissue sources showed positive expression of CD73 (NT5E), CD90 (THY1), CD105 (ENDOGLIN), and very low expression of CD45. The isolated canine MSCs were successfully differentiated into adipogenic and osteogenic lineages. The oil-red-O quantification and adipogenic gene expression (FAS, FabP4, and PPARγ) were higher in OM-derived cells, followed by IPFP-MSCs. Similarly, in osteogenic differentiation, alkaline phosphatase activity and osteogenic gene (SP7 and Osteocalcin) expression were higher in OM-derived MSCs, while osteopontin expression was higher in PR-derived MSCs. Conclusion In summary, among all four adipose tissue sources, OM-derived MSCs have better differentiation potential toward adipo- and osteogenic lineages, followed by IPFP-MSCs. Interestingly, among all adipose tissue sources, MSCs derived from IPFP have the maximum proliferation potential. The characterization and differentiation potential of canine MSCs isolated from four different adipose tissue sources are useful to assess their potential for application in regenerative medicine. |
| first_indexed | 2024-12-21T23:55:11Z |
| format | Article |
| id | doaj.art-24616473eb0d4a3795d1449c5086dc80 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-21T23:55:11Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-24616473eb0d4a3795d1449c5086dc802022-12-21T18:45:50ZengBMCBMC Veterinary Research1746-61482021-12-0117111210.1186/s12917-021-03100-8Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sourcesUsman Rashid0Arfan Yousaf1Muhammad Yaqoob2Evelyn Saba3Muhammad Moaeen-ud-Din4Shahid Waseem5Sandra K. Becker6Gerhard Sponder7Jörg R. Aschenbach8Mansur Abdullah Sandhu9Department of Clinical Studies, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture UniversityDepartment of Clinical Studies, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture UniversityDepartment of Clinical Studies, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture UniversityDepartment of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture UniversityDepartment of Animal Breeding and Genetics, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture UniversityABO ScientificInstitute of Veterinary-Physiology, Freie Universität BerlinInstitute of Veterinary-Physiology, Freie Universität BerlinInstitute of Veterinary-Physiology, Freie Universität BerlinDepartment of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture UniversityAbstract Background Mesenchymal stem cells (MSCs) are undifferentiated cells that can give rise to a mesoderm lineage. Adipose-derived MSCs are an easy and accessible source for MSCs isolation, although each source of MSC has its own advantages and disadvantages. Our study identifies a promising source for the isolation and differentiation of canines MSCs. For this purpose, adipose tissue from inguinal subcutaneous (SC), perirenal (PR), omental (OM), and infrapatellar fat pad (IPFP) was isolated and processed for MSCs isolation. In the third passage, MSCs proliferation/metabolism, surface markers expression, in vitro differentiation potential and quantitative reverse transcription PCR (CD73, CD90, CD105, PPARγ, FabP4, FAS, SP7, Osteopontin, and Osteocalcin) were evaluated. Results Our results showed that MSCs derived from IPFP have a higher proliferation rate, while OM-derived MSCs have higher cell metabolism. In addition, MSCs from all adipose tissue sources showed positive expression of CD73 (NT5E), CD90 (THY1), CD105 (ENDOGLIN), and very low expression of CD45. The isolated canine MSCs were successfully differentiated into adipogenic and osteogenic lineages. The oil-red-O quantification and adipogenic gene expression (FAS, FabP4, and PPARγ) were higher in OM-derived cells, followed by IPFP-MSCs. Similarly, in osteogenic differentiation, alkaline phosphatase activity and osteogenic gene (SP7 and Osteocalcin) expression were higher in OM-derived MSCs, while osteopontin expression was higher in PR-derived MSCs. Conclusion In summary, among all four adipose tissue sources, OM-derived MSCs have better differentiation potential toward adipo- and osteogenic lineages, followed by IPFP-MSCs. Interestingly, among all adipose tissue sources, MSCs derived from IPFP have the maximum proliferation potential. The characterization and differentiation potential of canine MSCs isolated from four different adipose tissue sources are useful to assess their potential for application in regenerative medicine.https://doi.org/10.1186/s12917-021-03100-8Mesenchymal stem cellAdipose tissueDogAdipogenesisOsteogenesis |
| spellingShingle | Usman Rashid Arfan Yousaf Muhammad Yaqoob Evelyn Saba Muhammad Moaeen-ud-Din Shahid Waseem Sandra K. Becker Gerhard Sponder Jörg R. Aschenbach Mansur Abdullah Sandhu Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources BMC Veterinary Research Mesenchymal stem cell Adipose tissue Dog Adipogenesis Osteogenesis |
| title | Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources |
| title_full | Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources |
| title_fullStr | Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources |
| title_full_unstemmed | Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources |
| title_short | Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources |
| title_sort | characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources |
| topic | Mesenchymal stem cell Adipose tissue Dog Adipogenesis Osteogenesis |
| url | https://doi.org/10.1186/s12917-021-03100-8 |
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