Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm Reactor
Partial denitrification, the termination of NO<sub>3</sub><sup>−</sup>-N reduction at nitrite (NO<sub>2</sub><sup>−</sup>-N), has received growing interest for treating wastewaters with high ammonium concentrations, because it can be coupled to anammox...
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MDPI AG
2022-05-01
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| author | Si Pang Bruce E. Rittmann Chengyang Wu Lin Yang Jingzhou Zhou Siqing Xia |
| author_facet | Si Pang Bruce E. Rittmann Chengyang Wu Lin Yang Jingzhou Zhou Siqing Xia |
| author_sort | Si Pang |
| collection | DOAJ |
| description | Partial denitrification, the termination of NO<sub>3</sub><sup>−</sup>-N reduction at nitrite (NO<sub>2</sub><sup>−</sup>-N), has received growing interest for treating wastewaters with high ammonium concentrations, because it can be coupled to anammox for total-nitrogen removal. NO<sub>2</sub><sup>−</sup> accumulation in the hydrogen (H<sub>2</sub>)-based membrane biofilm reactor (MBfR) has rarely been studied, and the mechanisms behind its accumulation have not been defined. This study aimed at achieving the partial denitrification with H<sub>2</sub>-based autotrophic reducing bacteria in a MBfR. Results showed that by increasing the NO<sub>3</sub><sup>−</sup> loading, increasing the pH, and decreasing the inorganic-carbon concentration, a nitrite transformation rate higher than 68% was achieved. Community analysis indicated that <i>Thauera</i> and <i>Azoarcus</i> became the dominant genera when partial denitrification was occurring. Functional genes abundances proved that partial denitrification to accumulate NO<sub>2</sub><sup>−</sup> was correlated to increases of gene for the form I RuBisCo enzyme (<i>cbbL</i>). This study confirmed the feasibility of autotrophic partial denitrification formed in the MBfR, and revealed the inorganic carbon mechanism in MBfR denitrification. |
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| language | English |
| last_indexed | 2024-03-10T03:19:11Z |
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| spelling | doaj.art-246218a4d70c42bfa0c0d3f60cb9ed8c2023-11-23T10:05:58ZengMDPI AGBioengineering2306-53542022-05-019522210.3390/bioengineering9050222Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm ReactorSi Pang0Bruce E. Rittmann1Chengyang Wu2Lin Yang3Jingzhou Zhou4Siqing Xia5State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, ChinaBiodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, AZ 85287, USAState Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, ChinaState Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, ChinaState Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, ChinaState Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, ChinaPartial denitrification, the termination of NO<sub>3</sub><sup>−</sup>-N reduction at nitrite (NO<sub>2</sub><sup>−</sup>-N), has received growing interest for treating wastewaters with high ammonium concentrations, because it can be coupled to anammox for total-nitrogen removal. NO<sub>2</sub><sup>−</sup> accumulation in the hydrogen (H<sub>2</sub>)-based membrane biofilm reactor (MBfR) has rarely been studied, and the mechanisms behind its accumulation have not been defined. This study aimed at achieving the partial denitrification with H<sub>2</sub>-based autotrophic reducing bacteria in a MBfR. Results showed that by increasing the NO<sub>3</sub><sup>−</sup> loading, increasing the pH, and decreasing the inorganic-carbon concentration, a nitrite transformation rate higher than 68% was achieved. Community analysis indicated that <i>Thauera</i> and <i>Azoarcus</i> became the dominant genera when partial denitrification was occurring. Functional genes abundances proved that partial denitrification to accumulate NO<sub>2</sub><sup>−</sup> was correlated to increases of gene for the form I RuBisCo enzyme (<i>cbbL</i>). This study confirmed the feasibility of autotrophic partial denitrification formed in the MBfR, and revealed the inorganic carbon mechanism in MBfR denitrification.https://www.mdpi.com/2306-5354/9/5/222membrane biofilm reactornitrite accumulationinorganic carbon fixing genesdenitrification genesmicrobial communityautotrophic partial denitrification |
| spellingShingle | Si Pang Bruce E. Rittmann Chengyang Wu Lin Yang Jingzhou Zhou Siqing Xia Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm Reactor Bioengineering membrane biofilm reactor nitrite accumulation inorganic carbon fixing genes denitrification genes microbial community autotrophic partial denitrification |
| title | Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm Reactor |
| title_full | Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm Reactor |
| title_fullStr | Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm Reactor |
| title_full_unstemmed | Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm Reactor |
| title_short | Synergistic Inorganic Carbon and Denitrification Genes Contributed to Nitrite Accumulation in a Hydrogen-Based Membrane Biofilm Reactor |
| title_sort | synergistic inorganic carbon and denitrification genes contributed to nitrite accumulation in a hydrogen based membrane biofilm reactor |
| topic | membrane biofilm reactor nitrite accumulation inorganic carbon fixing genes denitrification genes microbial community autotrophic partial denitrification |
| url | https://www.mdpi.com/2306-5354/9/5/222 |
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