The effect of topical ketamine administration on the corneal epithelium repair
Abstract The cornea is regarded as a sensitive organ to pain. Ketamine can effectively reduce postoperative neuropathic pain. We hypothesized that topical ketamine could mitigate postoperative corneal neuropathic pain. The aim of this study was to determine whether topical ketamine is safe for corne...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24639-y |
| _version_ | 1811292219494105088 |
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| author | Mehdi Sanatkar Zohre Nozarian Fatemeh Bazvand Parisa Abdi |
| author_facet | Mehdi Sanatkar Zohre Nozarian Fatemeh Bazvand Parisa Abdi |
| author_sort | Mehdi Sanatkar |
| collection | DOAJ |
| description | Abstract The cornea is regarded as a sensitive organ to pain. Ketamine can effectively reduce postoperative neuropathic pain. We hypothesized that topical ketamine could mitigate postoperative corneal neuropathic pain. The aim of this study was to determine whether topical ketamine is safe for cornea and evaluate its effect on the repair procedure the damaged corneal tissue. Our study was performed on only the right eyes of 15 male rats. All animals underwent general anesthesia and the whole corneal epithelium was removed. All subjects were divided into two groups: group 1 (n = 8), one drop of ketamine, and group 2 (n = 7), one drop of 0.9% sodium chloride administered topically on the scraped cornea every 6 h for 7 days. The rats’ s cornea was carefully monitored daily for the size of epithelial defects under a microscope and was photographed. On the eighth day, the eyes were sent for pathological examination. The eyes were examined for the amount of inflammation, neovascularization, keratinization, epithelial thickness and Descemet's membrane pathologies. The epithelial defect has healed completely on the sixth day in all rats in both groups. There was no significant difference in the speed of complete recovery between the two groups. No significant difference was observed between the two groups in terms of inflammation grade, neovascularization grade, and epithelial thickness. Our study showed that topical ketamine had no significant effect on corneal wound healing in a rat animal model and could be used safely for the management of postoperative chronic ocular pain. |
| first_indexed | 2024-04-13T04:42:07Z |
| format | Article |
| id | doaj.art-2462dd7d1552422484af59ae9a93d894 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T04:42:07Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2462dd7d1552422484af59ae9a93d8942022-12-22T03:01:57ZengNature PortfolioScientific Reports2045-23222022-12-011211710.1038/s41598-022-24639-yThe effect of topical ketamine administration on the corneal epithelium repairMehdi Sanatkar0Zohre Nozarian1Fatemeh Bazvand2Parisa Abdi3Anesthesiology, Critical Care and Pain Department, Farabi Eye Hospital, Tehran University of Medical SciencesPathology Department, Farabi Eye Hospital, Tehran University of Medical SciencesRetina Department, Farabi Eye Hospital, Tehran University of Medical SciencesCornea Department, Farabi Eye Hospital, Tehran University of Medical SciencesAbstract The cornea is regarded as a sensitive organ to pain. Ketamine can effectively reduce postoperative neuropathic pain. We hypothesized that topical ketamine could mitigate postoperative corneal neuropathic pain. The aim of this study was to determine whether topical ketamine is safe for cornea and evaluate its effect on the repair procedure the damaged corneal tissue. Our study was performed on only the right eyes of 15 male rats. All animals underwent general anesthesia and the whole corneal epithelium was removed. All subjects were divided into two groups: group 1 (n = 8), one drop of ketamine, and group 2 (n = 7), one drop of 0.9% sodium chloride administered topically on the scraped cornea every 6 h for 7 days. The rats’ s cornea was carefully monitored daily for the size of epithelial defects under a microscope and was photographed. On the eighth day, the eyes were sent for pathological examination. The eyes were examined for the amount of inflammation, neovascularization, keratinization, epithelial thickness and Descemet's membrane pathologies. The epithelial defect has healed completely on the sixth day in all rats in both groups. There was no significant difference in the speed of complete recovery between the two groups. No significant difference was observed between the two groups in terms of inflammation grade, neovascularization grade, and epithelial thickness. Our study showed that topical ketamine had no significant effect on corneal wound healing in a rat animal model and could be used safely for the management of postoperative chronic ocular pain.https://doi.org/10.1038/s41598-022-24639-y |
| spellingShingle | Mehdi Sanatkar Zohre Nozarian Fatemeh Bazvand Parisa Abdi The effect of topical ketamine administration on the corneal epithelium repair Scientific Reports |
| title | The effect of topical ketamine administration on the corneal epithelium repair |
| title_full | The effect of topical ketamine administration on the corneal epithelium repair |
| title_fullStr | The effect of topical ketamine administration on the corneal epithelium repair |
| title_full_unstemmed | The effect of topical ketamine administration on the corneal epithelium repair |
| title_short | The effect of topical ketamine administration on the corneal epithelium repair |
| title_sort | effect of topical ketamine administration on the corneal epithelium repair |
| url | https://doi.org/10.1038/s41598-022-24639-y |
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