Linkers for oligonucleotide microarray synthesis
A number of linkers for microchip-based (microarray) synthesis of oligonucleotides were synthesized here using photolabile intermediates. The resulting linkers are designed for covalent immobilization on slides containing both hydroxyl and amino groups.The first series of linkers was intended for th...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of Saudi Chemical Society |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319610321001873 |
| _version_ | 1798026688192315392 |
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| author | A.N. Sinyakov V.A. Ryabinin E.V. Kostina D.E. Zaytsev N.V. Chukanov G.N. Kamaev |
| author_facet | A.N. Sinyakov V.A. Ryabinin E.V. Kostina D.E. Zaytsev N.V. Chukanov G.N. Kamaev |
| author_sort | A.N. Sinyakov |
| collection | DOAJ |
| description | A number of linkers for microchip-based (microarray) synthesis of oligonucleotides were synthesized here using photolabile intermediates. The resulting linkers are designed for covalent immobilization on slides containing both hydroxyl and amino groups.The first series of linkers was intended for the synthesis of oligonucleotide arrays for hybridization analysis. These linkers provide a strong covalent bond with the slide surface when amino groups in oligonucleotide heterocyclic bases are deprotected.The second series of linkers allows to cleave the synthesized oligonucleotides from the support on which they were synthesized. Furthermore, the use of such linkers yields oligonucleotides devoid of the phosphate group at their 3′ end.The obtained linkers were successfully tested in the synthesis of oligonucleotides on a microchip for subsequent hybridization analysis and assembly of a DNA fragment. |
| first_indexed | 2024-04-11T18:39:25Z |
| format | Article |
| id | doaj.art-2463728a020241e5b1786dc87d91f3de |
| institution | Directory Open Access Journal |
| issn | 1319-6103 |
| language | English |
| last_indexed | 2024-04-11T18:39:25Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Saudi Chemical Society |
| spelling | doaj.art-2463728a020241e5b1786dc87d91f3de2022-12-22T04:09:03ZengElsevierJournal of Saudi Chemical Society1319-61032021-12-012512101382Linkers for oligonucleotide microarray synthesisA.N. Sinyakov0V.A. Ryabinin1E.V. Kostina2D.E. Zaytsev3N.V. Chukanov4G.N. Kamaev5Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, Russia; Corresponding author.Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, RussiaInternational Tomography Center, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, RussiaInstitute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, RussiaA number of linkers for microchip-based (microarray) synthesis of oligonucleotides were synthesized here using photolabile intermediates. The resulting linkers are designed for covalent immobilization on slides containing both hydroxyl and amino groups.The first series of linkers was intended for the synthesis of oligonucleotide arrays for hybridization analysis. These linkers provide a strong covalent bond with the slide surface when amino groups in oligonucleotide heterocyclic bases are deprotected.The second series of linkers allows to cleave the synthesized oligonucleotides from the support on which they were synthesized. Furthermore, the use of such linkers yields oligonucleotides devoid of the phosphate group at their 3′ end.The obtained linkers were successfully tested in the synthesis of oligonucleotides on a microchip for subsequent hybridization analysis and assembly of a DNA fragment.http://www.sciencedirect.com/science/article/pii/S1319610321001873SynthesisOligonucleotideHybridization analysisAssembly of an artificial genesDNA |
| spellingShingle | A.N. Sinyakov V.A. Ryabinin E.V. Kostina D.E. Zaytsev N.V. Chukanov G.N. Kamaev Linkers for oligonucleotide microarray synthesis Journal of Saudi Chemical Society Synthesis Oligonucleotide Hybridization analysis Assembly of an artificial genes DNA |
| title | Linkers for oligonucleotide microarray synthesis |
| title_full | Linkers for oligonucleotide microarray synthesis |
| title_fullStr | Linkers for oligonucleotide microarray synthesis |
| title_full_unstemmed | Linkers for oligonucleotide microarray synthesis |
| title_short | Linkers for oligonucleotide microarray synthesis |
| title_sort | linkers for oligonucleotide microarray synthesis |
| topic | Synthesis Oligonucleotide Hybridization analysis Assembly of an artificial genes DNA |
| url | http://www.sciencedirect.com/science/article/pii/S1319610321001873 |
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