Structural Elucidation of Secondary Metabolites in Sponge (Callyspongia pseudoreticulata) with N-Hexane Extract
Sponge is one of the marine species that has a high bioactivity and contains secondary metabobolite compound which can be used as antibiotic and medicine. Terpenoid compound derivatives have been successfully isolated from sponge Callyspongia pseudoretilulata by a method in which the n-hexane extrac...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hasanuddin University
2015-05-01
|
| Series: | International Journal of Agriculture System |
| Subjects: | |
| Online Access: | http://pasca.unhas.ac.id/ojs/index.php/ijas/article/view/23 |
| Summary: | Sponge is one of the marine species that has a high bioactivity and contains secondary metabobolite compound which can be used as antibiotic and medicine. Terpenoid compound derivatives have been successfully isolated from sponge Callyspongia pseudoretilulata by a method in which the n-hexane extract weighing 11.28 g was fractionated using vacuum column chromatography with a non-polar eluent, n-hexane. The polarity of this eluent was improved by ethyl acetic resulting in fairly polar eluent, methanol. A total of 27 fractions were obtained and one of the compounds from n-hexane extract was structurally elucidated by NMR spectroscopy and two dimensions of homonuclear and heteronuclear (1H, 13C, DEPT, H-H COSY, HMQC, and HMBC). The identified compound was a hydrocarbon (1-ethyl cyclo hex cosana-1-amin) that indicated LC50 of 60.58 against A. salina and has a great potential as antitumor or anticancer. |
|---|---|
| ISSN: | 2337-9782 2580-6815 |