The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum
The polysaccharides derived from various deproteinization methods were prepared from Flos Sophorae Immaturus (FSI) to investigate the prebiotic efficacy of Lactobacillus fermentum (L.f ). The implications of polysaccharides from FSI (PFSI) gained after purification performed by non-deproteinization...
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| Format: | Article |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1007267/full |
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| author | Wenting Zhong Wenting Zhong Chunmiao Yang Yongze Zhang Yongze Zhang Dongsheng Yang |
| author_facet | Wenting Zhong Wenting Zhong Chunmiao Yang Yongze Zhang Yongze Zhang Dongsheng Yang |
| author_sort | Wenting Zhong |
| collection | DOAJ |
| description | The polysaccharides derived from various deproteinization methods were prepared from Flos Sophorae Immaturus (FSI) to investigate the prebiotic efficacy of Lactobacillus fermentum (L.f ). The implications of polysaccharides from FSI (PFSI) gained after purification performed by non-deproteinization and different deproteinization processes (Savage method, papain method, and TCA method) via one-factor optimization were firstly investigated for the influences on the growth of L.f. The utilization of carbohydrate sources and the synthesis of protein and lactate during its growth were analyzed, as well as the variations of LDH, SOD, and GSH- Px enzyme dynamics. The results showed that the one-factor optimization of the deproteinization process with the protein removal rate and polysaccharide retention rate as the indexes led to the optimal methods of the Sevage method with 5 elution times, papain method with 80 U/mL concentration, and TCA method with 2.5 ratio, respectively. In addition, the PFSI obtained with or without deproteinization purification had a certain effect on promoting L.f proliferation. Moreover, the PFSI gained by the third deproteinization purification, at a concentration of 10 g/L, significantly elevated L.f biomass and growth rate compared with the blank control, and the utilization of reducing sugars and the synthesis of protein and lactic acid were higher than the control (P < 0.05); improved LDH, SOD, and GSH-Px activity in L.f (P < 0.05), and the TCA method could be effectively applied to eliminate the proteins affecting FSI in probiotics, and PFSI may be a potentially beneficial prebiotic and intestinal reinforcer. |
| first_indexed | 2024-04-13T18:16:46Z |
| format | Article |
| id | doaj.art-2469166f195a4ceda5f60094d409d55f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T18:16:46Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2469166f195a4ceda5f60094d409d55f2022-12-22T02:35:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10072671007267The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentumWenting Zhong0Wenting Zhong1Chunmiao Yang2Yongze Zhang3Yongze Zhang4Dongsheng Yang5College of Pharmacy and Food Science, Zhuhai College of Science and Technology, Zhuhai, ChinaCollege of Life Science, Jilin University, Changchun, ChinaCollege of Pharmacy and Food Science, Zhuhai College of Science and Technology, Zhuhai, ChinaCollege of Pharmacy and Food Science, Zhuhai College of Science and Technology, Zhuhai, ChinaCollege of Life Science, Jilin University, Changchun, ChinaCollege of Pharmacy and Food Science, Zhuhai College of Science and Technology, Zhuhai, ChinaThe polysaccharides derived from various deproteinization methods were prepared from Flos Sophorae Immaturus (FSI) to investigate the prebiotic efficacy of Lactobacillus fermentum (L.f ). The implications of polysaccharides from FSI (PFSI) gained after purification performed by non-deproteinization and different deproteinization processes (Savage method, papain method, and TCA method) via one-factor optimization were firstly investigated for the influences on the growth of L.f. The utilization of carbohydrate sources and the synthesis of protein and lactate during its growth were analyzed, as well as the variations of LDH, SOD, and GSH- Px enzyme dynamics. The results showed that the one-factor optimization of the deproteinization process with the protein removal rate and polysaccharide retention rate as the indexes led to the optimal methods of the Sevage method with 5 elution times, papain method with 80 U/mL concentration, and TCA method with 2.5 ratio, respectively. In addition, the PFSI obtained with or without deproteinization purification had a certain effect on promoting L.f proliferation. Moreover, the PFSI gained by the third deproteinization purification, at a concentration of 10 g/L, significantly elevated L.f biomass and growth rate compared with the blank control, and the utilization of reducing sugars and the synthesis of protein and lactic acid were higher than the control (P < 0.05); improved LDH, SOD, and GSH-Px activity in L.f (P < 0.05), and the TCA method could be effectively applied to eliminate the proteins affecting FSI in probiotics, and PFSI may be a potentially beneficial prebiotic and intestinal reinforcer.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1007267/fullLactobacillus fermentumFlos Sophorae Immaturuspolysaccharide deproteinizationTCA methodisolation and purificationphysiological properties of enzymes |
| spellingShingle | Wenting Zhong Wenting Zhong Chunmiao Yang Yongze Zhang Yongze Zhang Dongsheng Yang The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum Frontiers in Microbiology Lactobacillus fermentum Flos Sophorae Immaturus polysaccharide deproteinization TCA method isolation and purification physiological properties of enzymes |
| title | The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum |
| title_full | The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum |
| title_fullStr | The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum |
| title_full_unstemmed | The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum |
| title_short | The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum |
| title_sort | prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from flos sophorae immaturus on lactobacillus fermentum |
| topic | Lactobacillus fermentum Flos Sophorae Immaturus polysaccharide deproteinization TCA method isolation and purification physiological properties of enzymes |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1007267/full |
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