The design of natural hybrid biomaterial to promote osteogenic differentiation, collagen i and II expression and relief of musculoskeletal pains: Bone tissue-engineering applications (in-vitro and clinical studies)
This research aimed to fabricate a natural hybrid biomaterial and assess its physicochemical/biological properties to study the role of the therapeutic biomaterial in the growth of human monocytes, synoviocytes, chondrocytes, expression of type-I/II collagen, and osteogenic differentiation for bone...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535224001680 |
| _version_ | 1827256598407413760 |
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| author | Azadeh Izadyari Aghmiuni Saeed Heidari keshel Ali Aghababai Mohammad Zahraei Mostafa Rezaei-tavirani |
| author_facet | Azadeh Izadyari Aghmiuni Saeed Heidari keshel Ali Aghababai Mohammad Zahraei Mostafa Rezaei-tavirani |
| author_sort | Azadeh Izadyari Aghmiuni |
| collection | DOAJ |
| description | This research aimed to fabricate a natural hybrid biomaterial and assess its physicochemical/biological properties to study the role of the therapeutic biomaterial in the growth of human monocytes, synoviocytes, chondrocytes, expression of type-I/II collagen, and osteogenic differentiation for bone tissue engineering applications, as well as to assess effects of bio-ointment derived from this biomaterial on relief of musculoskeletal pains. Hence, an oily formula [turmeric:ginger:chili pepper:rose oils (1:1:1:1w/w)] was mixed with the base oil [sesame:black seed:olive oils (1:0.4:0.6w/w)] in 3 wt ratios of 1:6(H1), 1.5:5.5(H2), and 2:5(H3). Accordingly, H2 indicated a better performance in cell growth and decrease of inflammatory mediators and led to the highest expression of osteoblastic genes of RUNX2, OCN, and OPN, as well as collagen I and II (COL1A1,COL2A) respectively 1.3-fold, 1.7-fold, and 1.4-fold, as well as %87 % and ∼ 60 % higher than H1, and ∼ 2-fold, 2.1-fold, and 3.4-fold, and ∼ 19 % and ∼ 16 % higher than H3. A clinical study on H2 was also performed on 50 patients with musculoskeletal pains. H2-based bio-ointment could relieve pain in less than 10 min for 40 % of patients and after 30 min from use for 35 %. Moreover, the analgesia period for 60 % and 36 % of patients lasted for longer than 3 h and between 2 h and 3 h, respectively. 76 % also stated that bio-ointment possesses stronger effects than other treatments along with moderate warming functions. Finally, although 6 % of patients reported mild effectiveness in pain relief, 92 % of users stated this therapeutic method has resulted in more effects on their pain relief and damaged organ performance recovery. |
| first_indexed | 2024-04-24T12:50:48Z |
| format | Article |
| id | doaj.art-246c559807eb4791b83d7a435475f0b3 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2025-03-22T01:41:27Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-246c559807eb4791b83d7a435475f0b32024-05-09T04:35:41ZengElsevierArabian Journal of Chemistry1878-53522024-06-01176105766The design of natural hybrid biomaterial to promote osteogenic differentiation, collagen i and II expression and relief of musculoskeletal pains: Bone tissue-engineering applications (in-vitro and clinical studies)Azadeh Izadyari Aghmiuni0Saeed Heidari keshel1Ali Aghababai2Mohammad Zahraei3Mostafa Rezaei-tavirani4Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding authors.Department of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding authors.Department of Chemical Engineering, Shahrekord Branch, Islamic Azad University, Shahrekord, IranDepartment of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Tissue Engineering and Applied Cell Science, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranThis research aimed to fabricate a natural hybrid biomaterial and assess its physicochemical/biological properties to study the role of the therapeutic biomaterial in the growth of human monocytes, synoviocytes, chondrocytes, expression of type-I/II collagen, and osteogenic differentiation for bone tissue engineering applications, as well as to assess effects of bio-ointment derived from this biomaterial on relief of musculoskeletal pains. Hence, an oily formula [turmeric:ginger:chili pepper:rose oils (1:1:1:1w/w)] was mixed with the base oil [sesame:black seed:olive oils (1:0.4:0.6w/w)] in 3 wt ratios of 1:6(H1), 1.5:5.5(H2), and 2:5(H3). Accordingly, H2 indicated a better performance in cell growth and decrease of inflammatory mediators and led to the highest expression of osteoblastic genes of RUNX2, OCN, and OPN, as well as collagen I and II (COL1A1,COL2A) respectively 1.3-fold, 1.7-fold, and 1.4-fold, as well as %87 % and ∼ 60 % higher than H1, and ∼ 2-fold, 2.1-fold, and 3.4-fold, and ∼ 19 % and ∼ 16 % higher than H3. A clinical study on H2 was also performed on 50 patients with musculoskeletal pains. H2-based bio-ointment could relieve pain in less than 10 min for 40 % of patients and after 30 min from use for 35 %. Moreover, the analgesia period for 60 % and 36 % of patients lasted for longer than 3 h and between 2 h and 3 h, respectively. 76 % also stated that bio-ointment possesses stronger effects than other treatments along with moderate warming functions. Finally, although 6 % of patients reported mild effectiveness in pain relief, 92 % of users stated this therapeutic method has resulted in more effects on their pain relief and damaged organ performance recovery.http://www.sciencedirect.com/science/article/pii/S1878535224001680Natural biomaterialsOsteogenic differentiationMusculoskeletal painsNon-steroidal anti-inflammatory drugs (NSAIDs)Bone regenerationBio-ointment |
| spellingShingle | Azadeh Izadyari Aghmiuni Saeed Heidari keshel Ali Aghababai Mohammad Zahraei Mostafa Rezaei-tavirani The design of natural hybrid biomaterial to promote osteogenic differentiation, collagen i and II expression and relief of musculoskeletal pains: Bone tissue-engineering applications (in-vitro and clinical studies) Arabian Journal of Chemistry Natural biomaterials Osteogenic differentiation Musculoskeletal pains Non-steroidal anti-inflammatory drugs (NSAIDs) Bone regeneration Bio-ointment |
| title | The design of natural hybrid biomaterial to promote osteogenic differentiation, collagen i and II expression and relief of musculoskeletal pains: Bone tissue-engineering applications (in-vitro and clinical studies) |
| title_full | The design of natural hybrid biomaterial to promote osteogenic differentiation, collagen i and II expression and relief of musculoskeletal pains: Bone tissue-engineering applications (in-vitro and clinical studies) |
| title_fullStr | The design of natural hybrid biomaterial to promote osteogenic differentiation, collagen i and II expression and relief of musculoskeletal pains: Bone tissue-engineering applications (in-vitro and clinical studies) |
| title_full_unstemmed | The design of natural hybrid biomaterial to promote osteogenic differentiation, collagen i and II expression and relief of musculoskeletal pains: Bone tissue-engineering applications (in-vitro and clinical studies) |
| title_short | The design of natural hybrid biomaterial to promote osteogenic differentiation, collagen i and II expression and relief of musculoskeletal pains: Bone tissue-engineering applications (in-vitro and clinical studies) |
| title_sort | design of natural hybrid biomaterial to promote osteogenic differentiation collagen i and ii expression and relief of musculoskeletal pains bone tissue engineering applications in vitro and clinical studies |
| topic | Natural biomaterials Osteogenic differentiation Musculoskeletal pains Non-steroidal anti-inflammatory drugs (NSAIDs) Bone regeneration Bio-ointment |
| url | http://www.sciencedirect.com/science/article/pii/S1878535224001680 |
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