Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic
ABSTRACT: Necrotic enteritis (NE) is a poultry intestinal disease caused by virulent strains of the bacterium Clostridium perfringens (C. perfringens). This anaerobic bacterium produces a wide range of enzymes and toxins in the gut which leads to NE development. It is generally accepted by the poult...
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| Materialtyp: | Artikel |
| Språk: | English |
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Elsevier
2024-03-01
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| Serie: | Poultry Science |
| Ämnen: | |
| Länkar: | http://www.sciencedirect.com/science/article/pii/S0032579124000129 |
| _version_ | 1827338898753191936 |
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| author | Nicolas Deslauriers Lila Maduro Dion Lepp Joshua Gong Mohamed Faizal Abdul-Careem Martine Boulianne |
| author_facet | Nicolas Deslauriers Lila Maduro Dion Lepp Joshua Gong Mohamed Faizal Abdul-Careem Martine Boulianne |
| author_sort | Nicolas Deslauriers |
| collection | DOAJ |
| description | ABSTRACT: Necrotic enteritis (NE) is a poultry intestinal disease caused by virulent strains of the bacterium Clostridium perfringens (C. perfringens). This anaerobic bacterium produces a wide range of enzymes and toxins in the gut which leads to NE development. It is generally accepted by the poultry veterinarians that netB-positive C. perfringens strains are virulent and netB-negative strains do not cause NE. However, NE pathogenesis remains unclear as contradictory results have been reported. The use of experimental in vivo models is a valuable tool to understand the pathogenesis of a disease. In this study, a chicken ligated loop model was used to determine the virulence status of 79 C. perfringens strains from various geographical locations, sources, and genotype profiles. According to our model and based on histologic lesion scoring, 9 C. perfringens strains were classified as commensal, 35 as virulent, and 34 as highly virulent. The virulence of only 1 C. perfringens strain could not be classified as its lesion score was variable (from <10 to >15). In general, NE lesions were more severe in intestinal loops inoculated with netB-positive C. perfringens strains than those inoculated with netB-negative strains. The prevalence of netB among strains classified as commensal, virulent, and highly virulent was 56% (5/9), 54%, (19/35), and 59% (20/34). These results suggest that NetB is not required to cause NE lesions and that other factors are also involved. The classification of the virulence status of C. perfringens strains should not be based solely on the presence or absence of this toxin. Therefore, the use of an in vivo model is essential to distinguish commensal from virulent strains of C. perfringens. |
| first_indexed | 2024-03-07T19:43:13Z |
| format | Article |
| id | doaj.art-24765c57e3e94f3a9b0692dffd136d5f |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-07T19:43:13Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-24765c57e3e94f3a9b0692dffd136d5f2024-02-29T05:17:21ZengElsevierPoultry Science0032-57912024-03-011033103433Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necroticNicolas Deslauriers0Lila Maduro1Dion Lepp2Joshua Gong3Mohamed Faizal Abdul-Careem4Martine Boulianne5Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec, CanadaDepartment of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, Ontario, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, Ontario, CanadaFaculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C58, Calgary, Alberta, CanadaDepartment of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec, Canada; Corresponding author:ABSTRACT: Necrotic enteritis (NE) is a poultry intestinal disease caused by virulent strains of the bacterium Clostridium perfringens (C. perfringens). This anaerobic bacterium produces a wide range of enzymes and toxins in the gut which leads to NE development. It is generally accepted by the poultry veterinarians that netB-positive C. perfringens strains are virulent and netB-negative strains do not cause NE. However, NE pathogenesis remains unclear as contradictory results have been reported. The use of experimental in vivo models is a valuable tool to understand the pathogenesis of a disease. In this study, a chicken ligated loop model was used to determine the virulence status of 79 C. perfringens strains from various geographical locations, sources, and genotype profiles. According to our model and based on histologic lesion scoring, 9 C. perfringens strains were classified as commensal, 35 as virulent, and 34 as highly virulent. The virulence of only 1 C. perfringens strain could not be classified as its lesion score was variable (from <10 to >15). In general, NE lesions were more severe in intestinal loops inoculated with netB-positive C. perfringens strains than those inoculated with netB-negative strains. The prevalence of netB among strains classified as commensal, virulent, and highly virulent was 56% (5/9), 54%, (19/35), and 59% (20/34). These results suggest that NetB is not required to cause NE lesions and that other factors are also involved. The classification of the virulence status of C. perfringens strains should not be based solely on the presence or absence of this toxin. Therefore, the use of an in vivo model is essential to distinguish commensal from virulent strains of C. perfringens.http://www.sciencedirect.com/science/article/pii/S0032579124000129virulence statusClostridium perfringensin vivo modelnecrotic enteritisbroiler |
| spellingShingle | Nicolas Deslauriers Lila Maduro Dion Lepp Joshua Gong Mohamed Faizal Abdul-Careem Martine Boulianne Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic Poultry Science virulence status Clostridium perfringens in vivo model necrotic enteritis broiler |
| title | Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic |
| title_full | Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic |
| title_fullStr | Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic |
| title_full_unstemmed | Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic |
| title_short | Determination of the virulence status of Clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic |
| title_sort | determination of the virulence status of clostridium perfringens strains using a chicken intestinal ligated loop model is important for understanding the pathogenesis of necrotic |
| topic | virulence status Clostridium perfringens in vivo model necrotic enteritis broiler |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124000129 |
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