Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers
Background Poa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of...
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PeerJ Inc.
2016-09-01
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| Online Access: | https://peerj.com/articles/2489.pdf |
| _version_ | 1797419414004432896 |
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| author | Magdalena Szenejko Przemysław Śmietana Edyta Stępień |
| author_facet | Magdalena Szenejko Przemysław Śmietana Edyta Stępień |
| author_sort | Magdalena Szenejko |
| collection | DOAJ |
| description | Background Poa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link between the geographical origin of a given population and its assessed genetic variation. Methods The molecular markers RAPD and ISSR were used and their efficiency compared using, inter alia, statistical multivariate methods (UPGMA and PCA). Results The low value of Dice’s coefficient (0.369) along with the significantly high percentage of polymorphic products indicates a substantial degree of genetic diversity among the studied populations. Our results found a correlation between the geographical origin of the studied populations and their genetic variations. For ISSR, which proved to be the more effective method in that respect, we selected primers with the greatest differentiating powers correlating to geographical origin. Discussion The populations evaluated in this study were characterized by a high genetic diversity. This seems to confirm the hypothesis that ecotypes of P. pratensis originating from different regions of Central Europe with different terrain structures and habitat conditions can be a source of great genetic variability. |
| first_indexed | 2024-03-09T06:47:00Z |
| format | Article |
| id | doaj.art-2476f87e2fa5427587cbe5623ff41003 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:47:00Z |
| publishDate | 2016-09-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-2476f87e2fa5427587cbe5623ff410032023-12-03T10:33:54ZengPeerJ Inc.PeerJ2167-83592016-09-014e248910.7717/peerj.2489Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markersMagdalena Szenejko0Przemysław Śmietana1Edyta Stępień2Faculty of Biology, Department of Ecology and Environmental Protection, Institute for Research on Biodiversity, University of Szczecin, Szczecin, PolandFaculty of Biology, Department of Ecology and Environmental Protection, Institute for Research on Biodiversity, University of Szczecin, Szczecin, PolandDepartment of Plant Taxonomy and Phytogeography, Institute for Research on Biodiversity, Faculty of Biology, University of Szczecin, Szczecin, PolandBackground Poa pratensis is one of the most common species of meadow grass in Europe. Most cultivars of the species found in Poland were originally derived from its ecotypes. We compared the effectiveness of the RAPD and ISSR methods in assessing the genetic diversity of the selected populations of P. pratensis. We examined whether these methods could be useful for detecting a possible link between the geographical origin of a given population and its assessed genetic variation. Methods The molecular markers RAPD and ISSR were used and their efficiency compared using, inter alia, statistical multivariate methods (UPGMA and PCA). Results The low value of Dice’s coefficient (0.369) along with the significantly high percentage of polymorphic products indicates a substantial degree of genetic diversity among the studied populations. Our results found a correlation between the geographical origin of the studied populations and their genetic variations. For ISSR, which proved to be the more effective method in that respect, we selected primers with the greatest differentiating powers correlating to geographical origin. Discussion The populations evaluated in this study were characterized by a high genetic diversity. This seems to confirm the hypothesis that ecotypes of P. pratensis originating from different regions of Central Europe with different terrain structures and habitat conditions can be a source of great genetic variability.https://peerj.com/articles/2489.pdfEcotypesGenetic diversityMicrosatellite primersPCAPoa pratensisGeographical distribution |
| spellingShingle | Magdalena Szenejko Przemysław Śmietana Edyta Stępień Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers PeerJ Ecotypes Genetic diversity Microsatellite primers PCA Poa pratensis Geographical distribution |
| title | Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers |
| title_full | Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers |
| title_fullStr | Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers |
| title_full_unstemmed | Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers |
| title_short | Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers |
| title_sort | genetic diversity of poa pratensis l depending on geographical origin and compared with genetic markers |
| topic | Ecotypes Genetic diversity Microsatellite primers PCA Poa pratensis Geographical distribution |
| url | https://peerj.com/articles/2489.pdf |
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