Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding
Protoplast fusion between incongruent Solanum bulbocastanum and S. tuberosum haploids was accomplished to produce hybrids combining elite traits from both parents. We identified 11 somatic hybrids out of 42 regenerants analyzed through ISSR markers. Some hybrids had loss or gain of fragments compare...
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| Format: | Article |
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Institute of Experimental Botany of the Czech Academy of Sciences
2012-03-01
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| Series: | Biologia Plantarum |
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| Online Access: | https://bp.ueb.cas.cz/artkey/bpl-201201-0001_Interspecific-somatic-hybrids-between-Solanum-bulbocastanum-and-S-tuberosum-and-their-haploidizati.php |
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| author | M. Iovene R. Aversano S. Savarese I. Caruso A. Di Matteo T. Cardi L. Frusciante D. Carputo |
| author_facet | M. Iovene R. Aversano S. Savarese I. Caruso A. Di Matteo T. Cardi L. Frusciante D. Carputo |
| author_sort | M. Iovene |
| collection | DOAJ |
| description | Protoplast fusion between incongruent Solanum bulbocastanum and S. tuberosum haploids was accomplished to produce hybrids combining elite traits from both parents. We identified 11 somatic hybrids out of 42 regenerants analyzed through ISSR markers. Some hybrids had loss or gain of fragments compared to the parents, likely due to rearrangements and deletions of chromosome segments after fusion, and/or to somaclonal variation during hybrid regeneration. Increased heterotic vigor for some traits as well as high diversity was observed as the effect of both ploidy and fusion combination. Microsporogenesis analysis indicated the occurrence of multivalent configurations and several meiotic abnormalities, such as chromosomes bridges and various spindle orientations. Since all hybrids were sterile, in vitro anther culture was employed for haploidization as a possible strategy to overcome barriers to hybridizations. Haploids were obtained from all the tetraploid S. bulbocastanum (+) S. tuberosum somatic hybrids tested, although with differences in both the number of embryos per 100 anthers cultured and the number of differentiated green plantlets. This is the first report on the successful production of haploid plants from S. bulbocastanum (+) S. tuberosum hybrids. |
| first_indexed | 2025-03-20T22:53:26Z |
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| id | doaj.art-24779991c02e4bdaa41265ca0b51b51e |
| institution | Directory Open Access Journal |
| issn | 1573-8264 |
| language | English |
| last_indexed | 2025-03-20T22:53:26Z |
| publishDate | 2012-03-01 |
| publisher | Institute of Experimental Botany of the Czech Academy of Sciences |
| record_format | Article |
| series | Biologia Plantarum |
| spelling | doaj.art-24779991c02e4bdaa41265ca0b51b51e2024-08-05T08:36:45ZengInstitute of Experimental Botany of the Czech Academy of SciencesBiologia Plantarum1573-82642012-03-015611810.1007/s10535-012-0008-3bpl-201201-0001Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breedingM. Iovene0R. Aversano1S. Savarese2I. Caruso3A. Di Matteo4T. Cardi5L. Frusciante6D. Carputo7Institute of Plant Genetics, Res. Div. Bari, CNR-IGV, Bari, ItalyDepartment of Soil, Plant, Environmental and Animal Production Sciences, University of Naples Federico II, Portici, ItalyDepartment of Soil, Plant, Environmental and Animal Production Sciences, University of Naples Federico II, Portici, ItalyDepartment of Soil, Plant, Environmental and Animal Production Sciences, University of Naples Federico II, Portici, ItalyDepartment of Soil, Plant, Environmental and Animal Production Sciences, University of Naples Federico II, Portici, ItalyInstitute of Plant Genetics, Res. Div. Portici, CNR-IGV, Portici, ItalyDepartment of Soil, Plant, Environmental and Animal Production Sciences, University of Naples Federico II, Portici, ItalyDepartment of Soil, Plant, Environmental and Animal Production Sciences, University of Naples Federico II, Portici, ItalyProtoplast fusion between incongruent Solanum bulbocastanum and S. tuberosum haploids was accomplished to produce hybrids combining elite traits from both parents. We identified 11 somatic hybrids out of 42 regenerants analyzed through ISSR markers. Some hybrids had loss or gain of fragments compared to the parents, likely due to rearrangements and deletions of chromosome segments after fusion, and/or to somaclonal variation during hybrid regeneration. Increased heterotic vigor for some traits as well as high diversity was observed as the effect of both ploidy and fusion combination. Microsporogenesis analysis indicated the occurrence of multivalent configurations and several meiotic abnormalities, such as chromosomes bridges and various spindle orientations. Since all hybrids were sterile, in vitro anther culture was employed for haploidization as a possible strategy to overcome barriers to hybridizations. Haploids were obtained from all the tetraploid S. bulbocastanum (+) S. tuberosum somatic hybrids tested, although with differences in both the number of embryos per 100 anthers cultured and the number of differentiated green plantlets. This is the first report on the successful production of haploid plants from S. bulbocastanum (+) S. tuberosum hybrids.https://bp.ueb.cas.cz/artkey/bpl-201201-0001_Interspecific-somatic-hybrids-between-Solanum-bulbocastanum-and-S-tuberosum-and-their-haploidizati.phpandrogenesismicrosporogenesismolecular markerssomaclonal variation |
| spellingShingle | M. Iovene R. Aversano S. Savarese I. Caruso A. Di Matteo T. Cardi L. Frusciante D. Carputo Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding Biologia Plantarum androgenesis microsporogenesis molecular markers somaclonal variation |
| title | Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding |
| title_full | Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding |
| title_fullStr | Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding |
| title_full_unstemmed | Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding |
| title_short | Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding |
| title_sort | interspecific somatic hybrids between solanum bulbocastanum and s tuberosum and their haploidization for potato breeding |
| topic | androgenesis microsporogenesis molecular markers somaclonal variation |
| url | https://bp.ueb.cas.cz/artkey/bpl-201201-0001_Interspecific-somatic-hybrids-between-Solanum-bulbocastanum-and-S-tuberosum-and-their-haploidizati.php |
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