Colistin and Carbapenem-Resistant <i>Acinetobacter baumannii</i> Aci46 in Thailand: Genome Analysis and Antibiotic Resistance Profiling
Resistance to the last-line antibiotics against invasive Gram-negative bacterial infection is a rising concern in public health. Multidrug resistant (MDR) <i>Acinetobacter baumannii</i> Aci46 can resist colistin and carbapenems with a minimum inhibitory concentration of 512 µg/mL as dete...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/9/1054 |
| _version_ | 1797520444282109952 |
|---|---|
| author | Nalumon Thadtapong Soraya Chaturongakul Sunhapas Soodvilai Padungsri Dubbs |
| author_facet | Nalumon Thadtapong Soraya Chaturongakul Sunhapas Soodvilai Padungsri Dubbs |
| author_sort | Nalumon Thadtapong |
| collection | DOAJ |
| description | Resistance to the last-line antibiotics against invasive Gram-negative bacterial infection is a rising concern in public health. Multidrug resistant (MDR) <i>Acinetobacter baumannii</i> Aci46 can resist colistin and carbapenems with a minimum inhibitory concentration of 512 µg/mL as determined by microdilution method and shows no zone of inhibition by disk diffusion method. These phenotypic characteristics prompted us to further investigate the genotypic characteristics of Aci46. Next generation sequencing was applied in this study to obtain whole genome data. We determined that Aci46 belongs to Pasture ST2 and is phylogenetically clustered with international clone (IC) II as the predominant strain in Thailand. Interestingly, Aci46 is identical to Oxford ST1962 that previously has never been isolated in Thailand. Two plasmids were identified (pAci46a and pAci46b), neither of which harbors any antibiotic resistance genes but pAci46a carries a conjugational system (type 4 secretion system or T4SS). Comparative genomics with other polymyxin and carbapenem-resistant <i>A. baumannii</i> strains (AC30 and R14) identified shared features such as CzcCBA, encoding a cobalt/zinc/cadmium efflux RND transporter, as well as a drug transporter with a possible role in colistin and/or carbapenem resistance in <i>A. baumannii</i>. Single nucleotide polymorphism (SNP) analyses against MDR ACICU strain showed three novel mutations i.e., Glu229Asp, Pro200Leu, and Ala138Thr, in the polymyxin resistance component, PmrB. Overall, this study focused on Aci46 whole genome data analysis, its correlation with antibiotic resistance phenotypes, and the presence of potential virulence associated factors. |
| first_indexed | 2024-03-10T07:56:51Z |
| format | Article |
| id | doaj.art-247b018d4dda428da5f95c1d089b61d3 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T07:56:51Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-247b018d4dda428da5f95c1d089b61d32023-11-22T11:45:33ZengMDPI AGAntibiotics2079-63822021-08-01109105410.3390/antibiotics10091054Colistin and Carbapenem-Resistant <i>Acinetobacter baumannii</i> Aci46 in Thailand: Genome Analysis and Antibiotic Resistance ProfilingNalumon Thadtapong0Soraya Chaturongakul1Sunhapas Soodvilai2Padungsri Dubbs3Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandResistance to the last-line antibiotics against invasive Gram-negative bacterial infection is a rising concern in public health. Multidrug resistant (MDR) <i>Acinetobacter baumannii</i> Aci46 can resist colistin and carbapenems with a minimum inhibitory concentration of 512 µg/mL as determined by microdilution method and shows no zone of inhibition by disk diffusion method. These phenotypic characteristics prompted us to further investigate the genotypic characteristics of Aci46. Next generation sequencing was applied in this study to obtain whole genome data. We determined that Aci46 belongs to Pasture ST2 and is phylogenetically clustered with international clone (IC) II as the predominant strain in Thailand. Interestingly, Aci46 is identical to Oxford ST1962 that previously has never been isolated in Thailand. Two plasmids were identified (pAci46a and pAci46b), neither of which harbors any antibiotic resistance genes but pAci46a carries a conjugational system (type 4 secretion system or T4SS). Comparative genomics with other polymyxin and carbapenem-resistant <i>A. baumannii</i> strains (AC30 and R14) identified shared features such as CzcCBA, encoding a cobalt/zinc/cadmium efflux RND transporter, as well as a drug transporter with a possible role in colistin and/or carbapenem resistance in <i>A. baumannii</i>. Single nucleotide polymorphism (SNP) analyses against MDR ACICU strain showed three novel mutations i.e., Glu229Asp, Pro200Leu, and Ala138Thr, in the polymyxin resistance component, PmrB. Overall, this study focused on Aci46 whole genome data analysis, its correlation with antibiotic resistance phenotypes, and the presence of potential virulence associated factors.https://www.mdpi.com/2079-6382/10/9/1054<i>Acinetobacter baumannii</i>colistincarbapenemsmultidrug resistantWGS |
| spellingShingle | Nalumon Thadtapong Soraya Chaturongakul Sunhapas Soodvilai Padungsri Dubbs Colistin and Carbapenem-Resistant <i>Acinetobacter baumannii</i> Aci46 in Thailand: Genome Analysis and Antibiotic Resistance Profiling Antibiotics <i>Acinetobacter baumannii</i> colistin carbapenems multidrug resistant WGS |
| title | Colistin and Carbapenem-Resistant <i>Acinetobacter baumannii</i> Aci46 in Thailand: Genome Analysis and Antibiotic Resistance Profiling |
| title_full | Colistin and Carbapenem-Resistant <i>Acinetobacter baumannii</i> Aci46 in Thailand: Genome Analysis and Antibiotic Resistance Profiling |
| title_fullStr | Colistin and Carbapenem-Resistant <i>Acinetobacter baumannii</i> Aci46 in Thailand: Genome Analysis and Antibiotic Resistance Profiling |
| title_full_unstemmed | Colistin and Carbapenem-Resistant <i>Acinetobacter baumannii</i> Aci46 in Thailand: Genome Analysis and Antibiotic Resistance Profiling |
| title_short | Colistin and Carbapenem-Resistant <i>Acinetobacter baumannii</i> Aci46 in Thailand: Genome Analysis and Antibiotic Resistance Profiling |
| title_sort | colistin and carbapenem resistant i acinetobacter baumannii i aci46 in thailand genome analysis and antibiotic resistance profiling |
| topic | <i>Acinetobacter baumannii</i> colistin carbapenems multidrug resistant WGS |
| url | https://www.mdpi.com/2079-6382/10/9/1054 |
| work_keys_str_mv | AT nalumonthadtapong colistinandcarbapenemresistantiacinetobacterbaumanniiiaci46inthailandgenomeanalysisandantibioticresistanceprofiling AT sorayachaturongakul colistinandcarbapenemresistantiacinetobacterbaumanniiiaci46inthailandgenomeanalysisandantibioticresistanceprofiling AT sunhapassoodvilai colistinandcarbapenemresistantiacinetobacterbaumanniiiaci46inthailandgenomeanalysisandantibioticresistanceprofiling AT padungsridubbs colistinandcarbapenemresistantiacinetobacterbaumanniiiaci46inthailandgenomeanalysisandantibioticresistanceprofiling |