Registered report: the androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in man
The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative (PCFMFRI) seeks to address growing concerns about reproducibility in scientific research by conducting replications of recent papers in the field of prostate cancer. This Registered Report describes the proposed replicatio...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2015-09-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/1231.pdf |
| _version_ | 1797420245047050240 |
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| author | Denise Chronscinski Srujana Cherukeri Fraser Tan Nicole Perfito Joelle Lomax Elizabeth Iorns |
| author_facet | Denise Chronscinski Srujana Cherukeri Fraser Tan Nicole Perfito Joelle Lomax Elizabeth Iorns |
| author_sort | Denise Chronscinski |
| collection | DOAJ |
| description | The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative (PCFMFRI) seeks to address growing concerns about reproducibility in scientific research by conducting replications of recent papers in the field of prostate cancer. This Registered Report describes the proposed replication plan of key experiments from “The Androgen Receptor Induces a Distinct Transcriptional Program in Castration-Resistant Prostate Cancer in Man” by Sharma and colleagues (2013), published in Cancer Cell in 2013. Of thousands of targets for the androgen receptor (AR), the authors elucidated a subset of 16 core genes that were consistently downregulated with castration and re-emerged with castration resistance. These 16 AR binding sites were distinct from those observed in cells in culture. The authors suggested that cellular context can have dramatic effects on downstream transcriptional regulation of AR binding sites. The present study will attempt to replicate Fig. 7C by comparing gene expression of the 16 core genes identified by Sharma and colleagues in xenograft tumor tissue compared to androgen treated LNCaP cells in vitro. The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative is a collaboration between the Prostate Cancer Foundation, the Movember Initiative, and Science Exchange, and the results of the replications will be published by PeerJ. |
| first_indexed | 2024-03-09T06:58:51Z |
| format | Article |
| id | doaj.art-247de97c643d4e238eb9b5b169ea7390 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:58:51Z |
| publishDate | 2015-09-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-247de97c643d4e238eb9b5b169ea73902023-12-03T09:56:31ZengPeerJ Inc.PeerJ2167-83592015-09-013e123110.7717/peerj.1231Registered report: the androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in manDenise Chronscinski0Srujana Cherukeri1Fraser Tan2Nicole Perfito3Joelle Lomax4Elizabeth Iorns5Noble Life Sciences, Inc., Gaithersburg, MD, USANoble Life Sciences, Inc., Gaithersburg, MD, USAScience Exchange and The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative, Palo Alto, CA, USAScience Exchange and The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative, Palo Alto, CA, USAScience Exchange and The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative, Palo Alto, CA, USAScience Exchange and The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative, Palo Alto, CA, USAThe Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative (PCFMFRI) seeks to address growing concerns about reproducibility in scientific research by conducting replications of recent papers in the field of prostate cancer. This Registered Report describes the proposed replication plan of key experiments from “The Androgen Receptor Induces a Distinct Transcriptional Program in Castration-Resistant Prostate Cancer in Man” by Sharma and colleagues (2013), published in Cancer Cell in 2013. Of thousands of targets for the androgen receptor (AR), the authors elucidated a subset of 16 core genes that were consistently downregulated with castration and re-emerged with castration resistance. These 16 AR binding sites were distinct from those observed in cells in culture. The authors suggested that cellular context can have dramatic effects on downstream transcriptional regulation of AR binding sites. The present study will attempt to replicate Fig. 7C by comparing gene expression of the 16 core genes identified by Sharma and colleagues in xenograft tumor tissue compared to androgen treated LNCaP cells in vitro. The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative is a collaboration between the Prostate Cancer Foundation, the Movember Initiative, and Science Exchange, and the results of the replications will be published by PeerJ.https://peerj.com/articles/1231.pdfReproducibilityMethodologyAR signalingProstate cancerCastration-resistant prostate cancer |
| spellingShingle | Denise Chronscinski Srujana Cherukeri Fraser Tan Nicole Perfito Joelle Lomax Elizabeth Iorns Registered report: the androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in man PeerJ Reproducibility Methodology AR signaling Prostate cancer Castration-resistant prostate cancer |
| title | Registered report: the androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in man |
| title_full | Registered report: the androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in man |
| title_fullStr | Registered report: the androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in man |
| title_full_unstemmed | Registered report: the androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in man |
| title_short | Registered report: the androgen receptor induces a distinct transcriptional program in castration-resistant prostate cancer in man |
| title_sort | registered report the androgen receptor induces a distinct transcriptional program in castration resistant prostate cancer in man |
| topic | Reproducibility Methodology AR signaling Prostate cancer Castration-resistant prostate cancer |
| url | https://peerj.com/articles/1231.pdf |
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