Simple and Visible Detection of Novel Astroviruses Causing Fatal Gout in Goslings Using One-Step Reverse Transcription Polymerase Spiral Reaction Method

Simple and Visible Detection of Novel Astroviruses Causing Fatal Gout in Goslings Using One-Step Reverse Transcription Polymerase Spiral Reaction Method

In this study, a one-step isothermal method combining polymerase spiral reaction (PSR) with reverse transcription (RT-PSR) was established for rapid and specific detection of novel astroviruses causing fatal gout in goslings (N-GoAstV). The one-step RT-PSR was accomplished at the optimal temperature...

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Bibliographic Details
Main Authors: Jun Ji, Qinxi Chen, Zhengli Yu, Xin Xu, Xinhao Mu, Xiang Tian, Xiaoge Fu, Lunguang Yao, Yingzuo Bi, Qingmei Xie
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Veterinary Science
Subjects:
N-GoAstV
rapidity
simplicity
visible detection
RT-PSR
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2020.579432/full

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