Establishment of droplet digital PCR for quantitative detection of Listeria monocytogenes in food
ObjectiveA droplet digital polymerase chain reaction （ddPCR） method was developed for the rapid quantitative detection of Listeria monocytogenes in food.MethodsSpecific primers and probes for Listeria monocytogenes were screened. The constant value effect of ddPCR method and plate counting method wa...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2022-09-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202205012?st=article_issue |
| _version_ | 1797976837776736256 |
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| author | WEI Yongxin MA Dan DONG Yiyang WEI Haiyan LI Dan ZHANG Ximeng GUO Yunchang LI Weiwei PEI Xiaoyan SONG Yueqian |
| author_facet | WEI Yongxin MA Dan DONG Yiyang WEI Haiyan LI Dan ZHANG Ximeng GUO Yunchang LI Weiwei PEI Xiaoyan SONG Yueqian |
| author_sort | WEI Yongxin |
| collection | DOAJ |
| description | ObjectiveA droplet digital polymerase chain reaction （ddPCR） method was developed for the rapid quantitative detection of Listeria monocytogenes in food.MethodsSpecific primers and probes for Listeria monocytogenes were screened. The constant value effect of ddPCR method and plate counting method was compared through the detection of pure bacterial solution and artificially contaminated samples. The specificity， sensitivity and repeatability of ddPCR method were analyzed.ResultsThe ddPCR had the characteristics of excellent specificity， sensitivity and repeatability in Listeria monocytogenes detection. The limit of detection （LOD） and the limit of quantification （LOQ） in pure bacterial solution were 136 CFU/mL. The LOQ in squid ring and sausage samples was 240 CFU/g and 155 CFU/g. The coefficient of variation of ddPCR was less than 25% at each gradient level， and the relative deviation of the logarithm of ddPCR and plate counting was less than 30%.ConclusionThe established ddPCR method is rapid， accurate， sensitive and specific for the quantitative detection of Listeria monocytogenes in food. |
| first_indexed | 2024-04-11T04:57:20Z |
| format | Article |
| id | doaj.art-2482133070f941a69d371c97c376fb8c |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:57:20Z |
| publishDate | 2022-09-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-2482133070f941a69d371c97c376fb8c2022-12-26T07:26:21ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562022-09-0134593794210.13590/j.cjfh.2022.05.0121004-8456（2022）05-0937-06Establishment of droplet digital PCR for quantitative detection of Listeria monocytogenes in foodWEI Yongxin0MA Dan1DONG Yiyang2WEI Haiyan3LI Dan4ZHANG Ximeng5GUO Yunchang6LI Weiwei7PEI Xiaoyan8SONG Yueqian9Science and Technology Research Center of China Customs, Beijing 100026, ChinaScience and Technology Research Center of China Customs, Beijing 100026, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaScience and Technology Research Center of China Customs, Beijing 100026, ChinaScience and Technology Research Center of China Customs, Beijing 100026, ChinaScience and Technology Research Center of China Customs, Beijing 100026, ChinaChina National Center for Food Safety Risk Assessment,Beijing 100021, ChinaChina National Center for Food Safety Risk Assessment,Beijing 100021, ChinaInner Mongolia Yili Industrial Group Co., Ltd, Beijing 100070, ChinaScience and Technology Research Center of China Customs, Beijing 100026, ChinaObjectiveA droplet digital polymerase chain reaction （ddPCR） method was developed for the rapid quantitative detection of Listeria monocytogenes in food.MethodsSpecific primers and probes for Listeria monocytogenes were screened. The constant value effect of ddPCR method and plate counting method was compared through the detection of pure bacterial solution and artificially contaminated samples. The specificity， sensitivity and repeatability of ddPCR method were analyzed.ResultsThe ddPCR had the characteristics of excellent specificity， sensitivity and repeatability in Listeria monocytogenes detection. The limit of detection （LOD） and the limit of quantification （LOQ） in pure bacterial solution were 136 CFU/mL. The LOQ in squid ring and sausage samples was 240 CFU/g and 155 CFU/g. The coefficient of variation of ddPCR was less than 25% at each gradient level， and the relative deviation of the logarithm of ddPCR and plate counting was less than 30%.ConclusionThe established ddPCR method is rapid， accurate， sensitive and specific for the quantitative detection of Listeria monocytogenes in food.http://www.zgspws.com/zgspwszz/article/abstract/202205012?st=article_issuelisteria monocytogenesdroplet digital polymerase chain reactionlimit of quantificationlimit of detection |
| spellingShingle | WEI Yongxin MA Dan DONG Yiyang WEI Haiyan LI Dan ZHANG Ximeng GUO Yunchang LI Weiwei PEI Xiaoyan SONG Yueqian Establishment of droplet digital PCR for quantitative detection of Listeria monocytogenes in food Zhongguo shipin weisheng zazhi listeria monocytogenes droplet digital polymerase chain reaction limit of quantification limit of detection |
| title | Establishment of droplet digital PCR for quantitative detection of Listeria monocytogenes in food |
| title_full | Establishment of droplet digital PCR for quantitative detection of Listeria monocytogenes in food |
| title_fullStr | Establishment of droplet digital PCR for quantitative detection of Listeria monocytogenes in food |
| title_full_unstemmed | Establishment of droplet digital PCR for quantitative detection of Listeria monocytogenes in food |
| title_short | Establishment of droplet digital PCR for quantitative detection of Listeria monocytogenes in food |
| title_sort | establishment of droplet digital pcr for quantitative detection of listeria monocytogenes in food |
| topic | listeria monocytogenes droplet digital polymerase chain reaction limit of quantification limit of detection |
| url | http://www.zgspws.com/zgspwszz/article/abstract/202205012?st=article_issue |
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