Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores.
Salmonellosis is a major public health problem throughout the world. Thus, there is a huge need for diversified control strategies for Salmonella infections. In this work, we have assessed the potential use of Bacillus subtilis (B. subtilis) spores for the expression of a major protective antigen of...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5785212?pdf=render |
| _version_ | 1828450984999780352 |
|---|---|
| author | Xixi Dai Minggang Liu Kangcheng Pan Jinlong Yang |
| author_facet | Xixi Dai Minggang Liu Kangcheng Pan Jinlong Yang |
| author_sort | Xixi Dai |
| collection | DOAJ |
| description | Salmonellosis is a major public health problem throughout the world. Thus, there is a huge need for diversified control strategies for Salmonella infections. In this work, we have assessed the potential use of Bacillus subtilis (B. subtilis) spores for the expression of a major protective antigen of Salmonella serovar Pullorum, OmpC. The expression of OmpC on the surface of spores was determined by immunofluorescence microscopy. Mice immunized with recombinant spores expressing the OmpC antigen presented significant levels of OmpC-specific serum IgG and mucosal SIgA antibodies than in mice immunized with non-recombinant spores (p<0.01). In addition, oral immunization with recombinant spores was able to induce a significant level of protection in mice against lethal challenge with Salmonella serovar Typhimurium. These results suggest that B. subtilis spores have promising potential in the development of mucosal vaccines against Salmonella infections. |
| first_indexed | 2024-12-10T23:30:27Z |
| format | Article |
| id | doaj.art-2489834fd48047dc8604320fa2afbe9e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T23:30:27Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2489834fd48047dc8604320fa2afbe9e2022-12-22T01:29:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01131e019162710.1371/journal.pone.0191627Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores.Xixi DaiMinggang LiuKangcheng PanJinlong YangSalmonellosis is a major public health problem throughout the world. Thus, there is a huge need for diversified control strategies for Salmonella infections. In this work, we have assessed the potential use of Bacillus subtilis (B. subtilis) spores for the expression of a major protective antigen of Salmonella serovar Pullorum, OmpC. The expression of OmpC on the surface of spores was determined by immunofluorescence microscopy. Mice immunized with recombinant spores expressing the OmpC antigen presented significant levels of OmpC-specific serum IgG and mucosal SIgA antibodies than in mice immunized with non-recombinant spores (p<0.01). In addition, oral immunization with recombinant spores was able to induce a significant level of protection in mice against lethal challenge with Salmonella serovar Typhimurium. These results suggest that B. subtilis spores have promising potential in the development of mucosal vaccines against Salmonella infections.http://europepmc.org/articles/PMC5785212?pdf=render |
| spellingShingle | Xixi Dai Minggang Liu Kangcheng Pan Jinlong Yang Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores. PLoS ONE |
| title | Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores. |
| title_full | Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores. |
| title_fullStr | Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores. |
| title_full_unstemmed | Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores. |
| title_short | Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores. |
| title_sort | surface display of ompc of salmonella serovar pullorum on bacillus subtilis spores |
| url | http://europepmc.org/articles/PMC5785212?pdf=render |
| work_keys_str_mv | AT xixidai surfacedisplayofompcofsalmonellaserovarpullorumonbacillussubtilisspores AT minggangliu surfacedisplayofompcofsalmonellaserovarpullorumonbacillussubtilisspores AT kangchengpan surfacedisplayofompcofsalmonellaserovarpullorumonbacillussubtilisspores AT jinlongyang surfacedisplayofompcofsalmonellaserovarpullorumonbacillussubtilisspores |