Lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean-reversion.
The life-long supply of blood cells depends on the long-term function of hematopoietic stem cells (HSCs). HSCs are functionally defined by their multi-potency and self-renewal capacity. Because of their self-renewal capacity, HSCs were thought to have indefinite lifespans. However, there is increasi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-04-01
|
| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC3630147?pdf=render |
| _version_ | 1811268561758322688 |
|---|---|
| author | Hans B Sieburg Giulio Cattarossi Christa E Muller-Sieburg |
| author_facet | Hans B Sieburg Giulio Cattarossi Christa E Muller-Sieburg |
| author_sort | Hans B Sieburg |
| collection | DOAJ |
| description | The life-long supply of blood cells depends on the long-term function of hematopoietic stem cells (HSCs). HSCs are functionally defined by their multi-potency and self-renewal capacity. Because of their self-renewal capacity, HSCs were thought to have indefinite lifespans. However, there is increasing evidence that genetically identical HSCs differ in lifespan and that the lifespan of a HSC is predetermined and HSC-intrinsic. Lifespan is here defined as the time a HSC gives rise to all mature blood cells. This raises the intriguing question: what controls the lifespan of HSCs within the same animal, exposed to the same environment? We present here a new model based on reliability theory to account for the diversity of lifespans of HSCs. Using clonal repopulation experiments and computational-mathematical modeling, we tested how small-scale, molecular level, failures are dissipated at the HSC population level. We found that the best fit of the experimental data is provided by a model, where the repopulation failure kinetics of each HSC are largely anti-persistent, or mean-reverting, processes. Thus, failure rates repeatedly increase during population-wide division events and are counteracted and decreased by repair processes. In the long-run, a crossover from anti-persistent to persistent behavior occurs. The cross-over is due to a slow increase in the mean failure rate of self-renewal and leads to rapid clonal extinction. This suggests that the repair capacity of HSCs is self-limiting. Furthermore, we show that the lifespan of each HSC depends on the amplitudes and frequencies of fluctuations in the failure rate kinetics. Shorter and longer lived HSCs differ significantly in their pre-programmed ability to dissipate perturbations. A likely interpretation of these findings is that the lifespan of HSCs is determined by preprogrammed differences in repair capacity. |
| first_indexed | 2024-04-12T21:24:26Z |
| format | Article |
| id | doaj.art-248a5bb4e0934154b4fe4f9d6d78a50b |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-04-12T21:24:26Z |
| publishDate | 2013-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-248a5bb4e0934154b4fe4f9d6d78a50b2022-12-22T03:16:12ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582013-04-0194e100300610.1371/journal.pcbi.1003006Lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean-reversion.Hans B SieburgGiulio CattarossiChrista E Muller-SieburgThe life-long supply of blood cells depends on the long-term function of hematopoietic stem cells (HSCs). HSCs are functionally defined by their multi-potency and self-renewal capacity. Because of their self-renewal capacity, HSCs were thought to have indefinite lifespans. However, there is increasing evidence that genetically identical HSCs differ in lifespan and that the lifespan of a HSC is predetermined and HSC-intrinsic. Lifespan is here defined as the time a HSC gives rise to all mature blood cells. This raises the intriguing question: what controls the lifespan of HSCs within the same animal, exposed to the same environment? We present here a new model based on reliability theory to account for the diversity of lifespans of HSCs. Using clonal repopulation experiments and computational-mathematical modeling, we tested how small-scale, molecular level, failures are dissipated at the HSC population level. We found that the best fit of the experimental data is provided by a model, where the repopulation failure kinetics of each HSC are largely anti-persistent, or mean-reverting, processes. Thus, failure rates repeatedly increase during population-wide division events and are counteracted and decreased by repair processes. In the long-run, a crossover from anti-persistent to persistent behavior occurs. The cross-over is due to a slow increase in the mean failure rate of self-renewal and leads to rapid clonal extinction. This suggests that the repair capacity of HSCs is self-limiting. Furthermore, we show that the lifespan of each HSC depends on the amplitudes and frequencies of fluctuations in the failure rate kinetics. Shorter and longer lived HSCs differ significantly in their pre-programmed ability to dissipate perturbations. A likely interpretation of these findings is that the lifespan of HSCs is determined by preprogrammed differences in repair capacity.http://europepmc.org/articles/PMC3630147?pdf=render |
| spellingShingle | Hans B Sieburg Giulio Cattarossi Christa E Muller-Sieburg Lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean-reversion. PLoS Computational Biology |
| title | Lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean-reversion. |
| title_full | Lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean-reversion. |
| title_fullStr | Lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean-reversion. |
| title_full_unstemmed | Lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean-reversion. |
| title_short | Lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean-reversion. |
| title_sort | lifespan differences in hematopoietic stem cells are due to imperfect repair and unstable mean reversion |
| url | http://europepmc.org/articles/PMC3630147?pdf=render |
| work_keys_str_mv | AT hansbsieburg lifespandifferencesinhematopoieticstemcellsareduetoimperfectrepairandunstablemeanreversion AT giuliocattarossi lifespandifferencesinhematopoieticstemcellsareduetoimperfectrepairandunstablemeanreversion AT christaemullersieburg lifespandifferencesinhematopoieticstemcellsareduetoimperfectrepairandunstablemeanreversion |