Comparative insights into multiple drug resistance determinants in Stenotrophomonas maltophilia MER1
Objectives: Multidrug-resistant (MDR) Stenotrophomonas maltophilia strain MER1 was isolated from hospital wastewater in Shandong Province, China. This study aimed to determine the genetic determinants related to its striking MDR phenotype. Methods: Antimicrobial susceptibility testing of strain MER1...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716521001879 |
| _version_ | 1819100157647519744 |
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| author | Linlin Xie Aiping Zhou Jia Zhao Yuhang Tang Rui Zhao Yingping Zhou Guangxiang Cao Chuanqing Zhong Jun Li |
| author_facet | Linlin Xie Aiping Zhou Jia Zhao Yuhang Tang Rui Zhao Yingping Zhou Guangxiang Cao Chuanqing Zhong Jun Li |
| author_sort | Linlin Xie |
| collection | DOAJ |
| description | Objectives: Multidrug-resistant (MDR) Stenotrophomonas maltophilia strain MER1 was isolated from hospital wastewater in Shandong Province, China. This study aimed to determine the genetic determinants related to its striking MDR phenotype. Methods: Antimicrobial susceptibility testing of strain MER1 was performed by disk diffusion on Mueller–Hinton agar plates, and MICs were interpreted according to Clinical and Laboratory Standards Institute breakpoints. The genome of MER1 was sequenced and assembled using PacBio RS II and BGISEQ-500 platforms. Antimicrobial resistance determinants together with other transferability or adaptability determinants were identified by comparative genomics. Phylogenetic and contextual assays for these elements were conducted to assess the risk of spread of MER1. Results: Antimicrobial susceptibility testing revealed that strain MER1 is resistant to nine different antibiotics, including ampicillin, meropenem, amikacin, erythromycin, vancomycin, tetracycline, tigecycline, colistin and ceftazidime. Several genes were identified encoding efflux pumps and drug-inactivating agents, accounting for resistance to the above antibiotics, including meropenem, tigecycline and colistin regarded as last-line therapies for infections caused by MDR Gram-negative bacteria. MER1 co-harbours two non-mobile mcr homologues. A novel genomic region of variability was demonstrated to confer bacterial robustness and adaptability upon strain MER1. Conclusion: Collective efforts revealed the MDR properties and potential genetic determinants of S. maltophilia MER1 isolated from hospital wastewater. Comparative genomic analysis of S. maltophilia MER1 may provide insights into the prevention and treatment of antimicrobial-resistant infections. Our findings raise concern that the MDR genes in the reservoir of S. maltophilia may further spread into various ecological niches or medically high-risk pathogens. |
| first_indexed | 2024-12-22T00:58:19Z |
| format | Article |
| id | doaj.art-24907389a9d544a69564533aa518e76d |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-22T00:58:19Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-24907389a9d544a69564533aa518e76d2022-12-21T18:44:15ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-12-01272025Comparative insights into multiple drug resistance determinants in Stenotrophomonas maltophilia MER1Linlin Xie0Aiping Zhou1Jia Zhao2Yuhang Tang3Rui Zhao4Yingping Zhou5Guangxiang Cao6Chuanqing Zhong7Jun Li8College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China; Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, ChinaDepartment of Laboratory Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, ChinaSchool of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, ChinaCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, ChinaCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, ChinaSchool of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, ChinaCollege of Biomedical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, ChinaSchool of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, China; Corresponding authors.College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China; Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, China; Corresponding authors.Objectives: Multidrug-resistant (MDR) Stenotrophomonas maltophilia strain MER1 was isolated from hospital wastewater in Shandong Province, China. This study aimed to determine the genetic determinants related to its striking MDR phenotype. Methods: Antimicrobial susceptibility testing of strain MER1 was performed by disk diffusion on Mueller–Hinton agar plates, and MICs were interpreted according to Clinical and Laboratory Standards Institute breakpoints. The genome of MER1 was sequenced and assembled using PacBio RS II and BGISEQ-500 platforms. Antimicrobial resistance determinants together with other transferability or adaptability determinants were identified by comparative genomics. Phylogenetic and contextual assays for these elements were conducted to assess the risk of spread of MER1. Results: Antimicrobial susceptibility testing revealed that strain MER1 is resistant to nine different antibiotics, including ampicillin, meropenem, amikacin, erythromycin, vancomycin, tetracycline, tigecycline, colistin and ceftazidime. Several genes were identified encoding efflux pumps and drug-inactivating agents, accounting for resistance to the above antibiotics, including meropenem, tigecycline and colistin regarded as last-line therapies for infections caused by MDR Gram-negative bacteria. MER1 co-harbours two non-mobile mcr homologues. A novel genomic region of variability was demonstrated to confer bacterial robustness and adaptability upon strain MER1. Conclusion: Collective efforts revealed the MDR properties and potential genetic determinants of S. maltophilia MER1 isolated from hospital wastewater. Comparative genomic analysis of S. maltophilia MER1 may provide insights into the prevention and treatment of antimicrobial-resistant infections. Our findings raise concern that the MDR genes in the reservoir of S. maltophilia may further spread into various ecological niches or medically high-risk pathogens.http://www.sciencedirect.com/science/article/pii/S2213716521001879Comparative genomicsMultidrug resistanceCarbapenemsTigecyclinePolymyxinStenotrophomonas maltophilia |
| spellingShingle | Linlin Xie Aiping Zhou Jia Zhao Yuhang Tang Rui Zhao Yingping Zhou Guangxiang Cao Chuanqing Zhong Jun Li Comparative insights into multiple drug resistance determinants in Stenotrophomonas maltophilia MER1 Journal of Global Antimicrobial Resistance Comparative genomics Multidrug resistance Carbapenems Tigecycline Polymyxin Stenotrophomonas maltophilia |
| title | Comparative insights into multiple drug resistance determinants in Stenotrophomonas maltophilia MER1 |
| title_full | Comparative insights into multiple drug resistance determinants in Stenotrophomonas maltophilia MER1 |
| title_fullStr | Comparative insights into multiple drug resistance determinants in Stenotrophomonas maltophilia MER1 |
| title_full_unstemmed | Comparative insights into multiple drug resistance determinants in Stenotrophomonas maltophilia MER1 |
| title_short | Comparative insights into multiple drug resistance determinants in Stenotrophomonas maltophilia MER1 |
| title_sort | comparative insights into multiple drug resistance determinants in stenotrophomonas maltophilia mer1 |
| topic | Comparative genomics Multidrug resistance Carbapenems Tigecycline Polymyxin Stenotrophomonas maltophilia |
| url | http://www.sciencedirect.com/science/article/pii/S2213716521001879 |
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