FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein
N6-methyladenosine (m6A) modification can accommodate mRNA processing, stability, and translation in mammals, and fat mass and obesity associated protein (FTO) is a vital demethylase in the m6A modification pathway. <i>Clostridium perfringens type C</i> (<i>C</i><i>. pe...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/13/1644 |
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| author | Jiaojiao Yang Juanli Zhang Xiaoli Gao Ruirui Luo Kaihui Xie Wei Wang Jie Li Qiaoli Yang Xiaoyu Huang Zunqiang Yan Pengfei Wang Shuangbao Gun |
| author_facet | Jiaojiao Yang Juanli Zhang Xiaoli Gao Ruirui Luo Kaihui Xie Wei Wang Jie Li Qiaoli Yang Xiaoyu Huang Zunqiang Yan Pengfei Wang Shuangbao Gun |
| author_sort | Jiaojiao Yang |
| collection | DOAJ |
| description | N6-methyladenosine (m6A) modification can accommodate mRNA processing, stability, and translation in mammals, and fat mass and obesity associated protein (FTO) is a vital demethylase in the m6A modification pathway. <i>Clostridium perfringens type C</i> (<i>C</i><i>. perfringens type C</i>) causes diarrhea in piglets and has a serious impact on the pig industry. However, our understanding of the effect of m6A in the process of <i>C</i><i>. perfringens type C</i> infectious piglet diarrhea (CPTCIPD) is limited. Here, an in vitro model of CPTCIPD was constructed by treating the intestinal porcine epithelial cell line-J2 (IPEC-J2) with <i>Clostridium perfringens</i> beta2 (CPB2) toxin, and the role of FTO was analyzed using quantitative real-time polymerase chain reaction, Western blotting, and flow cytometry. The results revealed that the overall RNA m6A contents at the tissue and cell levels were significantly up-regulated after <i>C. perfringens</i> infection (<i>p</i> < 0.05). FTO expression was significantly reduced in CPB2-treated IPEC-J2 cells. Functionally, FTO knockdown in the treated cells inhibited their proliferation and promoted apoptosis and the inflammation phenotype, whereas FTO overexpression had the opposite effects. Inhibiting FTO prolonged the half-life and up-regulated the expression of Caspase 3, leading to apoptosis. Therefore, this work explored the regulation of FTO in IPEC-J2 cells after CPB2 treatment and enhanced our understanding of the effect of the m6A modification in CPTCIPD. |
| first_indexed | 2024-03-09T22:10:07Z |
| format | Article |
| id | doaj.art-24923120bebd4ae2a55eb915c0d419d8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:10:07Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-24923120bebd4ae2a55eb915c0d419d82023-11-23T19:32:24ZengMDPI AGAnimals2076-26152022-06-011213164410.3390/ani12131644FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic ProteinJiaojiao Yang0Juanli Zhang1Xiaoli Gao2Ruirui Luo3Kaihui Xie4Wei Wang5Jie Li6Qiaoli Yang7Xiaoyu Huang8Zunqiang Yan9Pengfei Wang10Shuangbao Gun11College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xi’an 712100, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaN6-methyladenosine (m6A) modification can accommodate mRNA processing, stability, and translation in mammals, and fat mass and obesity associated protein (FTO) is a vital demethylase in the m6A modification pathway. <i>Clostridium perfringens type C</i> (<i>C</i><i>. perfringens type C</i>) causes diarrhea in piglets and has a serious impact on the pig industry. However, our understanding of the effect of m6A in the process of <i>C</i><i>. perfringens type C</i> infectious piglet diarrhea (CPTCIPD) is limited. Here, an in vitro model of CPTCIPD was constructed by treating the intestinal porcine epithelial cell line-J2 (IPEC-J2) with <i>Clostridium perfringens</i> beta2 (CPB2) toxin, and the role of FTO was analyzed using quantitative real-time polymerase chain reaction, Western blotting, and flow cytometry. The results revealed that the overall RNA m6A contents at the tissue and cell levels were significantly up-regulated after <i>C. perfringens</i> infection (<i>p</i> < 0.05). FTO expression was significantly reduced in CPB2-treated IPEC-J2 cells. Functionally, FTO knockdown in the treated cells inhibited their proliferation and promoted apoptosis and the inflammation phenotype, whereas FTO overexpression had the opposite effects. Inhibiting FTO prolonged the half-life and up-regulated the expression of Caspase 3, leading to apoptosis. Therefore, this work explored the regulation of FTO in IPEC-J2 cells after CPB2 treatment and enhanced our understanding of the effect of the m6A modification in CPTCIPD.https://www.mdpi.com/2076-2615/12/13/1644m6Apiglet diarrheaIPEC-J2CPB2 toxinFTO |
| spellingShingle | Jiaojiao Yang Juanli Zhang Xiaoli Gao Ruirui Luo Kaihui Xie Wei Wang Jie Li Qiaoli Yang Xiaoyu Huang Zunqiang Yan Pengfei Wang Shuangbao Gun FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein Animals m6A piglet diarrhea IPEC-J2 CPB2 toxin FTO |
| title | FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein |
| title_full | FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein |
| title_fullStr | FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein |
| title_full_unstemmed | FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein |
| title_short | FTO Regulates Apoptosis in CPB2-Treated IPEC-J2 Cells by Targeting Caspase 3 Apoptotic Protein |
| title_sort | fto regulates apoptosis in cpb2 treated ipec j2 cells by targeting caspase 3 apoptotic protein |
| topic | m6A piglet diarrhea IPEC-J2 CPB2 toxin FTO |
| url | https://www.mdpi.com/2076-2615/12/13/1644 |
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