Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells
Objective:Most patients with non-muscle-invasive bladder cancer (NMIBC) do not respond to intravesical Bacillus Calmette-Guerin (BCG) immunotherapy and have high risk of NMIBC recurrence and progression. In addition to its therapeutic effect which increases the local immune response, BCG also exerts...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Galenos Yayinevi
2021-03-01
|
| Series: | Journal of Urological Surgery |
| Subjects: | |
| Online Access: |
http://jurolsurgery.org/archives/archive-detail/article-preview/bacillus-calmette-guerin-ncreases-base-excision-re/46830
|
| _version_ | 1828028194946547712 |
|---|---|
| author | Selçuk Keskin Berna Somuncu Meltem Müftüoğlu |
| author_facet | Selçuk Keskin Berna Somuncu Meltem Müftüoğlu |
| author_sort | Selçuk Keskin |
| collection | DOAJ |
| description | Objective:Most patients with non-muscle-invasive bladder cancer (NMIBC) do not respond to intravesical Bacillus Calmette-Guerin (BCG) immunotherapy and have high risk of NMIBC recurrence and progression. In addition to its therapeutic effect which increases the local immune response, BCG also exerts an anti-tumour effect by increasing oxidative stress, and producing reactive oxygen species and oxidative DNA damage in bladder cancer (BC) cells. The oxidative DNA damage is repaired by base excision repair (BER) mechanism. Thus, BER capacity of BC cells could be an important factor in response to BCG therapy. Effects of BCG on the activity of BER in BC transitional carcinoma cell line, T24 have been investigated.Materials and Methods:The uracil-initiated total BER and BER enzyme activities were measured in whole cell extracts with or without BCG treatment using a [γ-32P] adenosine triphosphate-labelled 51-mer DNA substrates.Results:BCG treatment increased the activities of uracil-initiated total BER and BER enzymes, uracil DNA glycosylase and DNA polymerase β in 6 h and 24 h repair periods and increased the activity of 8-oxoguanine DNA glycosylase in 6 h repair in T24 BC cell line.Conclusion:The enhanced BER activity in BC cells in response to BCG treatment could be an important factor in BCG resistance. |
| first_indexed | 2024-04-10T13:48:34Z |
| format | Article |
| id | doaj.art-249312aa0a1343558989874a2c37b000 |
| institution | Directory Open Access Journal |
| issn | 2148-9580 2148-9580 |
| language | English |
| last_indexed | 2024-04-10T13:48:34Z |
| publishDate | 2021-03-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Journal of Urological Surgery |
| spelling | doaj.art-249312aa0a1343558989874a2c37b0002023-02-15T16:10:50ZengGalenos YayineviJournal of Urological Surgery2148-95802148-95802021-03-018181210.4274/jus.galenos.2020.001313049054Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer CellsSelçuk Keskin0Berna Somuncu1Meltem Müftüoğlu2 Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Urology, İstanbul, Turkiye Acibadem Mehmet Ali Aydinlar University, Graduate School of Health Sciences, Department of Medical Biotechnology, İstanbul, Turkiye Acibadem Mehmet Ali Aydinlar University, Graduate School of Health Sciences, Department of Medical Biotechnology, İstanbul, Turkiye Objective:Most patients with non-muscle-invasive bladder cancer (NMIBC) do not respond to intravesical Bacillus Calmette-Guerin (BCG) immunotherapy and have high risk of NMIBC recurrence and progression. In addition to its therapeutic effect which increases the local immune response, BCG also exerts an anti-tumour effect by increasing oxidative stress, and producing reactive oxygen species and oxidative DNA damage in bladder cancer (BC) cells. The oxidative DNA damage is repaired by base excision repair (BER) mechanism. Thus, BER capacity of BC cells could be an important factor in response to BCG therapy. Effects of BCG on the activity of BER in BC transitional carcinoma cell line, T24 have been investigated.Materials and Methods:The uracil-initiated total BER and BER enzyme activities were measured in whole cell extracts with or without BCG treatment using a [γ-32P] adenosine triphosphate-labelled 51-mer DNA substrates.Results:BCG treatment increased the activities of uracil-initiated total BER and BER enzymes, uracil DNA glycosylase and DNA polymerase β in 6 h and 24 h repair periods and increased the activity of 8-oxoguanine DNA glycosylase in 6 h repair in T24 BC cell line.Conclusion:The enhanced BER activity in BC cells in response to BCG treatment could be an important factor in BCG resistance. http://jurolsurgery.org/archives/archive-detail/article-preview/bacillus-calmette-guerin-ncreases-base-excision-re/46830 base excision repairbladder cancerbacillus calmette-guerin |
| spellingShingle | Selçuk Keskin Berna Somuncu Meltem Müftüoğlu Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells Journal of Urological Surgery base excision repair bladder cancer bacillus calmette-guerin |
| title | Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells |
| title_full | Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells |
| title_fullStr | Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells |
| title_full_unstemmed | Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells |
| title_short | Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells |
| title_sort | bacillus calmette guerin increases base excision repair in bladder cancer cells |
| topic | base excision repair bladder cancer bacillus calmette-guerin |
| url |
http://jurolsurgery.org/archives/archive-detail/article-preview/bacillus-calmette-guerin-ncreases-base-excision-re/46830
|
| work_keys_str_mv | AT selcukkeskin bacilluscalmetteguerinincreasesbaseexcisionrepairinbladdercancercells AT bernasomuncu bacilluscalmetteguerinincreasesbaseexcisionrepairinbladdercancercells AT meltemmuftuoglu bacilluscalmetteguerinincreasesbaseexcisionrepairinbladdercancercells |