Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432
Skeletal myogenesis is a highly ordered process finely regulated by various factors. Long non-coding RNAs play an important regulatory role in myogenesis via multiple mechanisms. In this study, we identified the lncRNA Gm10561, which was upregulated during myogenic differentiation and is highly expr...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2022.2105052 |
| _version_ | 1797678802230312960 |
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| author | Shanshan Wang Baohua Tan Liyao Xiao Jiekang Zeng Xinming Zhao Linjun Hong Zicong Li Gengyuan Cai Enqin Zheng Ting Gu Zhenfang Wu |
| author_facet | Shanshan Wang Baohua Tan Liyao Xiao Jiekang Zeng Xinming Zhao Linjun Hong Zicong Li Gengyuan Cai Enqin Zheng Ting Gu Zhenfang Wu |
| author_sort | Shanshan Wang |
| collection | DOAJ |
| description | Skeletal myogenesis is a highly ordered process finely regulated by various factors. Long non-coding RNAs play an important regulatory role in myogenesis via multiple mechanisms. In this study, we identified the lncRNA Gm10561, which was upregulated during myogenic differentiation and is highly expressed in skeletal muscle. Knockdown of Gm10561 inhibited the proliferation and differentiation of C2C12 myoblasts in vitro and muscle growth in vivo. Overexpression of Gm10561 promoted the proliferation and differentiation of both C2C12 myoblasts and porcine muscle satellite cells. Notably, lncRNA Gm10561 is localized in the cytoplasm and competitively bound to miR-432, which directly targets MEF2C and E2F3. It was confirmed that lncRNA Gm10561 regulates the proliferation and differentiation of myoblasts by acting as a sponge of miR-432 to modulate MEF2C and E2F3 expression. Thus, the lncRNA-Gm10561-miR-432-MEF2C/E2F3 axis plays an important role in myogenesis. |
| first_indexed | 2024-03-11T23:05:13Z |
| format | Article |
| id | doaj.art-2499af598cbc48718f624df1d5fb02a9 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:05:13Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-2499af598cbc48718f624df1d5fb02a92023-09-21T13:23:12ZengTaylor & Francis GroupEpigenetics1559-22941559-23082022-12-0117132039205510.1080/15592294.2022.21050522105052Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432Shanshan Wang0Baohua Tan1Liyao Xiao2Jiekang Zeng3Xinming Zhao4Linjun Hong5Zicong Li6Gengyuan Cai7Enqin Zheng8Ting Gu9Zhenfang Wu10National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversityNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural UniversitySkeletal myogenesis is a highly ordered process finely regulated by various factors. Long non-coding RNAs play an important regulatory role in myogenesis via multiple mechanisms. In this study, we identified the lncRNA Gm10561, which was upregulated during myogenic differentiation and is highly expressed in skeletal muscle. Knockdown of Gm10561 inhibited the proliferation and differentiation of C2C12 myoblasts in vitro and muscle growth in vivo. Overexpression of Gm10561 promoted the proliferation and differentiation of both C2C12 myoblasts and porcine muscle satellite cells. Notably, lncRNA Gm10561 is localized in the cytoplasm and competitively bound to miR-432, which directly targets MEF2C and E2F3. It was confirmed that lncRNA Gm10561 regulates the proliferation and differentiation of myoblasts by acting as a sponge of miR-432 to modulate MEF2C and E2F3 expression. Thus, the lncRNA-Gm10561-miR-432-MEF2C/E2F3 axis plays an important role in myogenesis.http://dx.doi.org/10.1080/15592294.2022.2105052skeletal myogenesislncrnamirnaepigenetics |
| spellingShingle | Shanshan Wang Baohua Tan Liyao Xiao Jiekang Zeng Xinming Zhao Linjun Hong Zicong Li Gengyuan Cai Enqin Zheng Ting Gu Zhenfang Wu Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432 Epigenetics skeletal myogenesis lncrna mirna epigenetics |
| title | Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432 |
| title_full | Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432 |
| title_fullStr | Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432 |
| title_full_unstemmed | Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432 |
| title_short | Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432 |
| title_sort | long non coding rna gm10561 promotes myogenesis by sponging mir 432 |
| topic | skeletal myogenesis lncrna mirna epigenetics |
| url | http://dx.doi.org/10.1080/15592294.2022.2105052 |
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