Microemulsion-based approach for oral delivery of insulin: formulation design and characterization
Oral delivery of insulin provides a good alternative because it is non-invasive and patient-friendly. However, multiple challenges affected this route. To overcome barriers for oral delivery of insulin, we aimed to develop a novel insulin-loaded microemulsion system based on snail mucin for oral adm...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-03-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020304953 |
| _version_ | 1818316136435941376 |
|---|---|
| author | Mumuni A. Momoh Kenechukwu C. Franklin Chinazom P. Agbo Calister E. Ugwu Musiliu O. Adedokun Ofomatah C. Anthony Omeje E. Chidozie Augustine N. Okorie |
| author_facet | Mumuni A. Momoh Kenechukwu C. Franklin Chinazom P. Agbo Calister E. Ugwu Musiliu O. Adedokun Ofomatah C. Anthony Omeje E. Chidozie Augustine N. Okorie |
| author_sort | Mumuni A. Momoh |
| collection | DOAJ |
| description | Oral delivery of insulin provides a good alternative because it is non-invasive and patient-friendly. However, multiple challenges affected this route. To overcome barriers for oral delivery of insulin, we aimed to develop a novel insulin-loaded microemulsion system based on snail mucin for oral administration. The strategy in the novel system of using mucin loading insulin into the inner core of prepared water in oil microemulsion to provide sustained released, increased in vivo stability and enhanced drug absorption in the gastrointestinal tract. We report how microemulsion composed of varying ratios of snail mucin and Tween® 80 (1:9–9:1) using oil/water emulsion preparation method influenced insulin performance after oral administration. The results obtained include an encapsulation efficiency of above 70 %; in vitro release was sustained over 10 h and in vivo evaluations in diabetic rat model shows that insulin-loaded microencapsulation effectively reduced blood glucose levels over a period >8 h after oral administration. Therefore, we suggest that the developed formulation for oral insulin can be a promising alternative dosage form for oral protein delivery. |
| first_indexed | 2024-12-13T09:16:38Z |
| format | Article |
| id | doaj.art-249c32b96d314a79ae9ba2052f10018e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-13T09:16:38Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-249c32b96d314a79ae9ba2052f10018e2022-12-21T23:52:49ZengElsevierHeliyon2405-84402020-03-0163e03650Microemulsion-based approach for oral delivery of insulin: formulation design and characterizationMumuni A. Momoh0Kenechukwu C. Franklin1Chinazom P. Agbo2Calister E. Ugwu3Musiliu O. Adedokun4Ofomatah C. Anthony5Omeje E. Chidozie6Augustine N. Okorie7Drug Delivery Research Unit, Department of Pharmaceutics, Faculty of Pharmaceutical Sciences University of Nigeria Nsukka, Enugu State, Nigeria; Corresponding author.Drug Delivery Research Unit, Department of Pharmaceutics, Faculty of Pharmaceutical Sciences University of Nigeria Nsukka, Enugu State, NigeriaDrug Delivery Research Unit, Department of Pharmaceutics, Faculty of Pharmaceutical Sciences University of Nigeria Nsukka, Enugu State, NigeriaDepartment of Pharmaceutical Technology and Industrial Pharmacy, University of Nigeria Nsukka, Enugu State, NigeriaDepartment of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy University of Uyo, Akwa-Ibom State, NigeriaNational Centre for Energy Research and Development, University of Nigeria Nsukka, NigeriaDrug Delivery Research Unit, Department of Pharmaceutics, Faculty of Pharmaceutical Sciences University of Nigeria Nsukka, Enugu State, NigeriaDepartment of Pharmacology and Toxicology University of University of Nigeria Nsukka, Enugu State, NigeriaOral delivery of insulin provides a good alternative because it is non-invasive and patient-friendly. However, multiple challenges affected this route. To overcome barriers for oral delivery of insulin, we aimed to develop a novel insulin-loaded microemulsion system based on snail mucin for oral administration. The strategy in the novel system of using mucin loading insulin into the inner core of prepared water in oil microemulsion to provide sustained released, increased in vivo stability and enhanced drug absorption in the gastrointestinal tract. We report how microemulsion composed of varying ratios of snail mucin and Tween® 80 (1:9–9:1) using oil/water emulsion preparation method influenced insulin performance after oral administration. The results obtained include an encapsulation efficiency of above 70 %; in vitro release was sustained over 10 h and in vivo evaluations in diabetic rat model shows that insulin-loaded microencapsulation effectively reduced blood glucose levels over a period >8 h after oral administration. Therefore, we suggest that the developed formulation for oral insulin can be a promising alternative dosage form for oral protein delivery.http://www.sciencedirect.com/science/article/pii/S2405844020304953BiotechnologyMaterials applicationMaterials characterizationPharmaceutical chemistryBiological sciencesPharmacology |
| spellingShingle | Mumuni A. Momoh Kenechukwu C. Franklin Chinazom P. Agbo Calister E. Ugwu Musiliu O. Adedokun Ofomatah C. Anthony Omeje E. Chidozie Augustine N. Okorie Microemulsion-based approach for oral delivery of insulin: formulation design and characterization Heliyon Biotechnology Materials application Materials characterization Pharmaceutical chemistry Biological sciences Pharmacology |
| title | Microemulsion-based approach for oral delivery of insulin: formulation design and characterization |
| title_full | Microemulsion-based approach for oral delivery of insulin: formulation design and characterization |
| title_fullStr | Microemulsion-based approach for oral delivery of insulin: formulation design and characterization |
| title_full_unstemmed | Microemulsion-based approach for oral delivery of insulin: formulation design and characterization |
| title_short | Microemulsion-based approach for oral delivery of insulin: formulation design and characterization |
| title_sort | microemulsion based approach for oral delivery of insulin formulation design and characterization |
| topic | Biotechnology Materials application Materials characterization Pharmaceutical chemistry Biological sciences Pharmacology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020304953 |
| work_keys_str_mv | AT mumuniamomoh microemulsionbasedapproachfororaldeliveryofinsulinformulationdesignandcharacterization AT kenechukwucfranklin microemulsionbasedapproachfororaldeliveryofinsulinformulationdesignandcharacterization AT chinazompagbo microemulsionbasedapproachfororaldeliveryofinsulinformulationdesignandcharacterization AT calistereugwu microemulsionbasedapproachfororaldeliveryofinsulinformulationdesignandcharacterization AT musiliuoadedokun microemulsionbasedapproachfororaldeliveryofinsulinformulationdesignandcharacterization AT ofomatahcanthony microemulsionbasedapproachfororaldeliveryofinsulinformulationdesignandcharacterization AT omejeechidozie microemulsionbasedapproachfororaldeliveryofinsulinformulationdesignandcharacterization AT augustinenokorie microemulsionbasedapproachfororaldeliveryofinsulinformulationdesignandcharacterization |