The role of magnesium supplementation in cisplatin-induced nephrotoxicity in a rat model: No nephroprotectant effect
Objectives: Cisplatin (CP) is used as the commonest drug to treat solid tumors. It is accompanied by a nephrotoxicity side effect. The main objective of this study is to investigate the protective role of magnesium (Mg) supplementation in CP-induced nephrotoxicity in a rat model. Methods: Twenty-nin...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2012-01-01
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| Series: | International Journal of Preventive Medicine |
| Subjects: | |
| Online Access: | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2012;volume=3;issue=9;spage=637;epage=643;aulast=Ashrafi |
| _version_ | 1819075059040387072 |
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| author | Farzaneh Ashrafi Sara Haghshenas Mehdi Nematbakhsh Hamid Nasri Ardeshir Talebi Fatemeh Eshraghi-Jazi Zahra Pezeshki Tahereh Safari |
| author_facet | Farzaneh Ashrafi Sara Haghshenas Mehdi Nematbakhsh Hamid Nasri Ardeshir Talebi Fatemeh Eshraghi-Jazi Zahra Pezeshki Tahereh Safari |
| author_sort | Farzaneh Ashrafi |
| collection | DOAJ |
| description | Objectives: Cisplatin (CP) is used as the commonest drug to treat solid tumors. It is accompanied by a nephrotoxicity side effect. The main objective of this study is to investigate the protective role of magnesium (Mg) supplementation in CP-induced nephrotoxicity in a rat model.
Methods: Twenty-nine Wistar rats were randomly assigned to four groups (1-4). Groups 1-3 received 20, 80, and 200 mg/kg magnesium sulfate respectively, for 10 days, but on day 3, a single dose of CP (7 mg/kg, i.p.) was also injected. Group 4 (positive control group) received the same regimen of Groups 1-3 except saline instead magnesium sulfate. One week after CP administration, blood samples were obtained and all animals were killed for kidney histopathological investigations.
Results: All CP-treated animals lost weight, and the percentage of weight loss in Group 1 (low dose Mg sulfate treated) was significantly higher compared with the positive control group (Group 4, P < 0.05). The increase in blood urea nitrogen (BUN) and creatinine (Cr) levels in serum in Group 1 were more than those in other groups ( P < 0.05). No statistical differences were observed in serum magnesium, nitrite, and total protein levels among the groups. The kidney tissue damage in Groups 1-3 was not significantly different when compared with Group 4. Moreover, the kidney and testis weights in Group 1 were significantly greater than those in the positive control group (P < 0.05).
Conclusion: Regarding the BUN and Cr levels in the serum, kidneys weight, and the histopathological study, the low dose of Mg supplementation intensifies kidney toxicity and renal dysfunction in CP-induced nephrotoxicity in the rat model. However, the protective role of Mg with moderate and high doses is not certain. |
| first_indexed | 2024-12-21T18:19:23Z |
| format | Article |
| id | doaj.art-249ca4622d474c08a30bb9f59d39b187 |
| institution | Directory Open Access Journal |
| issn | 2008-7802 2008-8213 |
| language | English |
| last_indexed | 2024-12-21T18:19:23Z |
| publishDate | 2012-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Preventive Medicine |
| spelling | doaj.art-249ca4622d474c08a30bb9f59d39b1872022-12-21T18:54:34ZengWolters Kluwer Medknow PublicationsInternational Journal of Preventive Medicine2008-78022008-82132012-01-0139637643The role of magnesium supplementation in cisplatin-induced nephrotoxicity in a rat model: No nephroprotectant effectFarzaneh AshrafiSara HaghshenasMehdi NematbakhshHamid NasriArdeshir TalebiFatemeh Eshraghi-JaziZahra PezeshkiTahereh SafariObjectives: Cisplatin (CP) is used as the commonest drug to treat solid tumors. It is accompanied by a nephrotoxicity side effect. The main objective of this study is to investigate the protective role of magnesium (Mg) supplementation in CP-induced nephrotoxicity in a rat model. Methods: Twenty-nine Wistar rats were randomly assigned to four groups (1-4). Groups 1-3 received 20, 80, and 200 mg/kg magnesium sulfate respectively, for 10 days, but on day 3, a single dose of CP (7 mg/kg, i.p.) was also injected. Group 4 (positive control group) received the same regimen of Groups 1-3 except saline instead magnesium sulfate. One week after CP administration, blood samples were obtained and all animals were killed for kidney histopathological investigations. Results: All CP-treated animals lost weight, and the percentage of weight loss in Group 1 (low dose Mg sulfate treated) was significantly higher compared with the positive control group (Group 4, P < 0.05). The increase in blood urea nitrogen (BUN) and creatinine (Cr) levels in serum in Group 1 were more than those in other groups ( P < 0.05). No statistical differences were observed in serum magnesium, nitrite, and total protein levels among the groups. The kidney tissue damage in Groups 1-3 was not significantly different when compared with Group 4. Moreover, the kidney and testis weights in Group 1 were significantly greater than those in the positive control group (P < 0.05). Conclusion: Regarding the BUN and Cr levels in the serum, kidneys weight, and the histopathological study, the low dose of Mg supplementation intensifies kidney toxicity and renal dysfunction in CP-induced nephrotoxicity in the rat model. However, the protective role of Mg with moderate and high doses is not certain.http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2012;volume=3;issue=9;spage=637;epage=643;aulast=AshrafiCisplatinmagnesiumnephrotoxicityrat |
| spellingShingle | Farzaneh Ashrafi Sara Haghshenas Mehdi Nematbakhsh Hamid Nasri Ardeshir Talebi Fatemeh Eshraghi-Jazi Zahra Pezeshki Tahereh Safari The role of magnesium supplementation in cisplatin-induced nephrotoxicity in a rat model: No nephroprotectant effect International Journal of Preventive Medicine Cisplatin magnesium nephrotoxicity rat |
| title | The role of magnesium supplementation in cisplatin-induced nephrotoxicity in a rat model: No nephroprotectant effect |
| title_full | The role of magnesium supplementation in cisplatin-induced nephrotoxicity in a rat model: No nephroprotectant effect |
| title_fullStr | The role of magnesium supplementation in cisplatin-induced nephrotoxicity in a rat model: No nephroprotectant effect |
| title_full_unstemmed | The role of magnesium supplementation in cisplatin-induced nephrotoxicity in a rat model: No nephroprotectant effect |
| title_short | The role of magnesium supplementation in cisplatin-induced nephrotoxicity in a rat model: No nephroprotectant effect |
| title_sort | role of magnesium supplementation in cisplatin induced nephrotoxicity in a rat model no nephroprotectant effect |
| topic | Cisplatin magnesium nephrotoxicity rat |
| url | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2012;volume=3;issue=9;spage=637;epage=643;aulast=Ashrafi |
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