Key Components of Human Myofibre Denervation and Neuromuscular Junction Stability are Modulated by Age and Exercise
The decline in muscle mass and function with age is partly caused by a loss of muscle fibres through denervation. The purpose of this study was to investigate the potential of exercise to influence molecular targets involved in neuromuscular junction (NMJ) stability in healthy elderly individuals. P...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/4/893 |
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| author | Casper Soendenbroe Cecilie J. L. Bechshøft Mette F. Heisterberg Simon M. Jensen Emma Bomme Peter Schjerling Anders Karlsen Michael Kjaer Jesper L. Andersen Abigail L. Mackey |
| author_facet | Casper Soendenbroe Cecilie J. L. Bechshøft Mette F. Heisterberg Simon M. Jensen Emma Bomme Peter Schjerling Anders Karlsen Michael Kjaer Jesper L. Andersen Abigail L. Mackey |
| author_sort | Casper Soendenbroe |
| collection | DOAJ |
| description | The decline in muscle mass and function with age is partly caused by a loss of muscle fibres through denervation. The purpose of this study was to investigate the potential of exercise to influence molecular targets involved in neuromuscular junction (NMJ) stability in healthy elderly individuals. Participants from two studies (one group of 12 young and 12 elderly females and another group of 25 elderly males) performed a unilateral bout of resistance exercise. Muscle biopsies were collected at 4.5 h and up to 7 days post exercise for tissue analysis and cell culture. Molecular targets related to denervation and NMJ stability were analysed by immunohistochemistry and real-time reverse transcription polymerase chain reaction. In addition to a greater presence of denervated fibres, the muscle samples and cultured myotubes from the elderly individuals displayed altered gene expression levels of acetylcholine receptor (AChR) subunits. A single bout of exercise induced general changes in AChR subunit gene expression within the biopsy sampling timeframe, suggesting a sustained plasticity of the NMJ in elderly individuals. These data support the role of exercise in maintaining NMJ stability, even in elderly inactive individuals. Furthermore, the cell culture findings suggest that the transcriptional capacity of satellite cells for AChR subunit genes is negatively affected by ageing. |
| first_indexed | 2024-03-10T20:38:30Z |
| format | Article |
| id | doaj.art-249caa572f7746798ebf1054820408e5 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T20:38:30Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-249caa572f7746798ebf1054820408e52023-11-19T20:48:47ZengMDPI AGCells2073-44092020-04-019489310.3390/cells9040893Key Components of Human Myofibre Denervation and Neuromuscular Junction Stability are Modulated by Age and ExerciseCasper Soendenbroe0Cecilie J. L. Bechshøft1Mette F. Heisterberg2Simon M. Jensen3Emma Bomme4Peter Schjerling5Anders Karlsen6Michael Kjaer7Jesper L. Andersen8Abigail L. Mackey9Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital, Building 8, Nielsine Nielsens vej 11, 2400 Copenhagen NV, DenmarkThe decline in muscle mass and function with age is partly caused by a loss of muscle fibres through denervation. The purpose of this study was to investigate the potential of exercise to influence molecular targets involved in neuromuscular junction (NMJ) stability in healthy elderly individuals. Participants from two studies (one group of 12 young and 12 elderly females and another group of 25 elderly males) performed a unilateral bout of resistance exercise. Muscle biopsies were collected at 4.5 h and up to 7 days post exercise for tissue analysis and cell culture. Molecular targets related to denervation and NMJ stability were analysed by immunohistochemistry and real-time reverse transcription polymerase chain reaction. In addition to a greater presence of denervated fibres, the muscle samples and cultured myotubes from the elderly individuals displayed altered gene expression levels of acetylcholine receptor (AChR) subunits. A single bout of exercise induced general changes in AChR subunit gene expression within the biopsy sampling timeframe, suggesting a sustained plasticity of the NMJ in elderly individuals. These data support the role of exercise in maintaining NMJ stability, even in elderly inactive individuals. Furthermore, the cell culture findings suggest that the transcriptional capacity of satellite cells for AChR subunit genes is negatively affected by ageing.https://www.mdpi.com/2073-4409/9/4/893sarcopeniadenervationneuromuscular junctionheavy resistance exerciseacetylcholine receptorcell culture |
| spellingShingle | Casper Soendenbroe Cecilie J. L. Bechshøft Mette F. Heisterberg Simon M. Jensen Emma Bomme Peter Schjerling Anders Karlsen Michael Kjaer Jesper L. Andersen Abigail L. Mackey Key Components of Human Myofibre Denervation and Neuromuscular Junction Stability are Modulated by Age and Exercise Cells sarcopenia denervation neuromuscular junction heavy resistance exercise acetylcholine receptor cell culture |
| title | Key Components of Human Myofibre Denervation and Neuromuscular Junction Stability are Modulated by Age and Exercise |
| title_full | Key Components of Human Myofibre Denervation and Neuromuscular Junction Stability are Modulated by Age and Exercise |
| title_fullStr | Key Components of Human Myofibre Denervation and Neuromuscular Junction Stability are Modulated by Age and Exercise |
| title_full_unstemmed | Key Components of Human Myofibre Denervation and Neuromuscular Junction Stability are Modulated by Age and Exercise |
| title_short | Key Components of Human Myofibre Denervation and Neuromuscular Junction Stability are Modulated by Age and Exercise |
| title_sort | key components of human myofibre denervation and neuromuscular junction stability are modulated by age and exercise |
| topic | sarcopenia denervation neuromuscular junction heavy resistance exercise acetylcholine receptor cell culture |
| url | https://www.mdpi.com/2073-4409/9/4/893 |
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