Astrocytes enhance the invasion potential of glioblastoma stem-like cells.
Glioblastomas (GBMs) are characterized as highly invasive; the contribution of GBM stem-like cells (GSCs) to the invasive phenotype, however, has not been completely defined. Towards this end, we have defined the invasion potential of CD133+ GSCs and their differentiated CD133- counterparts grown un...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3551925?pdf=render |
| _version_ | 1819039424395083776 |
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| author | Barbara H Rath Joshlean M Fair Muhammad Jamal Kevin Camphausen Philip J Tofilon |
| author_facet | Barbara H Rath Joshlean M Fair Muhammad Jamal Kevin Camphausen Philip J Tofilon |
| author_sort | Barbara H Rath |
| collection | DOAJ |
| description | Glioblastomas (GBMs) are characterized as highly invasive; the contribution of GBM stem-like cells (GSCs) to the invasive phenotype, however, has not been completely defined. Towards this end, we have defined the invasion potential of CD133+ GSCs and their differentiated CD133- counterparts grown under standard in vitro conditions and in co-culture with astrocytes. Using a trans-well assay, astrocytes or astrocyte conditioned media in the bottom chamber significantly increased the invasion of GSCs yet had no effect on CD133- cells. In addition, a monolayer invasion assay showed that the GSCs invaded farther into an astrocyte monolayer than their differentiated progeny. Gene expression profiles were generated from two GSC lines grown in trans-well culture with astrocytes in the bottom chamber or directly in contact with astrocyte monolayers. In each co-culture model, genes whose expression was commonly increased in both GSC lines involved cell movement and included a number of genes that have been previously associated with tumor cell invasion. Similar gene expression modifications were not detected in CD133- cells co-cultured under the same conditions with astrocytes. Finally, evaluation of the secretome of astrocytes grown in monolayer identified a number of chemokines and cytokines associated with tumor cell invasion. These data suggest that astrocytes enhance the invasion of CD133+ GSCs and provide additional support for a critical role of brain microenvironment in the regulation of GBM biology. |
| first_indexed | 2024-12-21T08:52:59Z |
| format | Article |
| id | doaj.art-24a034c2741b48c2952d33c251a378a8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T08:52:59Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-24a034c2741b48c2952d33c251a378a82022-12-21T19:09:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5475210.1371/journal.pone.0054752Astrocytes enhance the invasion potential of glioblastoma stem-like cells.Barbara H RathJoshlean M FairMuhammad JamalKevin CamphausenPhilip J TofilonGlioblastomas (GBMs) are characterized as highly invasive; the contribution of GBM stem-like cells (GSCs) to the invasive phenotype, however, has not been completely defined. Towards this end, we have defined the invasion potential of CD133+ GSCs and their differentiated CD133- counterparts grown under standard in vitro conditions and in co-culture with astrocytes. Using a trans-well assay, astrocytes or astrocyte conditioned media in the bottom chamber significantly increased the invasion of GSCs yet had no effect on CD133- cells. In addition, a monolayer invasion assay showed that the GSCs invaded farther into an astrocyte monolayer than their differentiated progeny. Gene expression profiles were generated from two GSC lines grown in trans-well culture with astrocytes in the bottom chamber or directly in contact with astrocyte monolayers. In each co-culture model, genes whose expression was commonly increased in both GSC lines involved cell movement and included a number of genes that have been previously associated with tumor cell invasion. Similar gene expression modifications were not detected in CD133- cells co-cultured under the same conditions with astrocytes. Finally, evaluation of the secretome of astrocytes grown in monolayer identified a number of chemokines and cytokines associated with tumor cell invasion. These data suggest that astrocytes enhance the invasion of CD133+ GSCs and provide additional support for a critical role of brain microenvironment in the regulation of GBM biology.http://europepmc.org/articles/PMC3551925?pdf=render |
| spellingShingle | Barbara H Rath Joshlean M Fair Muhammad Jamal Kevin Camphausen Philip J Tofilon Astrocytes enhance the invasion potential of glioblastoma stem-like cells. PLoS ONE |
| title | Astrocytes enhance the invasion potential of glioblastoma stem-like cells. |
| title_full | Astrocytes enhance the invasion potential of glioblastoma stem-like cells. |
| title_fullStr | Astrocytes enhance the invasion potential of glioblastoma stem-like cells. |
| title_full_unstemmed | Astrocytes enhance the invasion potential of glioblastoma stem-like cells. |
| title_short | Astrocytes enhance the invasion potential of glioblastoma stem-like cells. |
| title_sort | astrocytes enhance the invasion potential of glioblastoma stem like cells |
| url | http://europepmc.org/articles/PMC3551925?pdf=render |
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