The Properties of Wet Blue Added Crude Enzyme from Rhizopus oligosporus in the Acid Bating Process

Bating is one of the processes in leather production. This process is generally performed under slightly alkaline conditions, but it can also be done under acidic conditions. Rhizopus oligosporus is categorized as a microorganism that can synthesize acidic enzymes optimally in acidic conditions. Thi...

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Main Authors: A. W. Nugraha, O. Suparno, N. S. Indrasti, Hoerudin
Format: Article
Language:English
Published: IPB University 2022-02-01
Series:Tropical Animal Science Journal
Subjects:
Online Access:https://jli.journal.ipb.ac.id/index.php/tasj/article/view/34972
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author A. W. Nugraha
O. Suparno
N. S. Indrasti
Hoerudin
author_facet A. W. Nugraha
O. Suparno
N. S. Indrasti
Hoerudin
author_sort A. W. Nugraha
collection DOAJ
description Bating is one of the processes in leather production. This process is generally performed under slightly alkaline conditions, but it can also be done under acidic conditions. Rhizopus oligosporus is categorized as a microorganism that can synthesize acidic enzymes optimally in acidic conditions. This study aimed to evaluate the effect of the bating process using acid protease from R. oligosporus on the leather qualities tanned by chromium sulfate. The study used a completely randomized design where crude enzyme activity (0, 2.5, 5, 7.5, and 10 U mL-1) was a single treatment as an experimental design. Data were analyzed using ANOVA and furtherly tested using Duncan. The results show that the protease synthesized from R. oligosporus is potential to be used as a bating agent. The treatment with 2.5 U mL-1 activity was the best treatment in this study to improve leather (wet blue) properties like shrinkage temperature, ash content, and chrome oxide content. The quality of wet blue produced was better than that produced with conventional treatments. It can be concluded that the enzyme activity of 2.5 U mL-1 could be used as a bating-agent alternative in the acid bating stage.
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spelling doaj.art-24a376492fdc4973b8540404cf9c90352022-12-22T00:04:46ZengIPB UniversityTropical Animal Science Journal2615-787X2615-790X2022-02-0145110.5398/tasj.2022.45.1.104The Properties of Wet Blue Added Crude Enzyme from Rhizopus oligosporus in the Acid Bating ProcessA. W. Nugraha0O. Suparno1N. S. Indrasti2Hoerudin3Department of Agro-Industrial Technology, Institut Teknologi SumateraDepartment of Agro-Industrial Technology, Faculty of Agricultural Technology, IPB UniversityDepartment of Agro-Industrial Technology, Faculty of Agricultural Technology, IPB UniversityIndonesian Center for Agricultural Post Harvest Research and DevelopmentBating is one of the processes in leather production. This process is generally performed under slightly alkaline conditions, but it can also be done under acidic conditions. Rhizopus oligosporus is categorized as a microorganism that can synthesize acidic enzymes optimally in acidic conditions. This study aimed to evaluate the effect of the bating process using acid protease from R. oligosporus on the leather qualities tanned by chromium sulfate. The study used a completely randomized design where crude enzyme activity (0, 2.5, 5, 7.5, and 10 U mL-1) was a single treatment as an experimental design. Data were analyzed using ANOVA and furtherly tested using Duncan. The results show that the protease synthesized from R. oligosporus is potential to be used as a bating agent. The treatment with 2.5 U mL-1 activity was the best treatment in this study to improve leather (wet blue) properties like shrinkage temperature, ash content, and chrome oxide content. The quality of wet blue produced was better than that produced with conventional treatments. It can be concluded that the enzyme activity of 2.5 U mL-1 could be used as a bating-agent alternative in the acid bating stage.https://jli.journal.ipb.ac.id/index.php/tasj/article/view/34972batingenzyme activityleather productionRhizopus oligosporus
spellingShingle A. W. Nugraha
O. Suparno
N. S. Indrasti
Hoerudin
The Properties of Wet Blue Added Crude Enzyme from Rhizopus oligosporus in the Acid Bating Process
Tropical Animal Science Journal
bating
enzyme activity
leather production
Rhizopus oligosporus
title The Properties of Wet Blue Added Crude Enzyme from Rhizopus oligosporus in the Acid Bating Process
title_full The Properties of Wet Blue Added Crude Enzyme from Rhizopus oligosporus in the Acid Bating Process
title_fullStr The Properties of Wet Blue Added Crude Enzyme from Rhizopus oligosporus in the Acid Bating Process
title_full_unstemmed The Properties of Wet Blue Added Crude Enzyme from Rhizopus oligosporus in the Acid Bating Process
title_short The Properties of Wet Blue Added Crude Enzyme from Rhizopus oligosporus in the Acid Bating Process
title_sort properties of wet blue added crude enzyme from rhizopus oligosporus in the acid bating process
topic bating
enzyme activity
leather production
Rhizopus oligosporus
url https://jli.journal.ipb.ac.id/index.php/tasj/article/view/34972
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