A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions
Panax notoginseng possesses therapeutic potential for cardiovascular disorders. Endoplasmic reticulum (ER) stress plays a critical role in cardiovascular disease. Here we show that a rare ginsenoside-standardized extract (RGSE) from notoginseng exerts cardioprotection via attenuating ER stress-assoc...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615001917 |
| _version_ | 1819294271478431744 |
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| author | Li-Chao Wang Wen-Song Zhang Qun Liu Jia Li Raphael N. Alolga Kang Liu Bao-Lin Liu Ping Li Lian-Wen Qi |
| author_facet | Li-Chao Wang Wen-Song Zhang Qun Liu Jia Li Raphael N. Alolga Kang Liu Bao-Lin Liu Ping Li Lian-Wen Qi |
| author_sort | Li-Chao Wang |
| collection | DOAJ |
| description | Panax notoginseng possesses therapeutic potential for cardiovascular disorders. Endoplasmic reticulum (ER) stress plays a critical role in cardiovascular disease. Here we show that a rare ginsenoside-standardized extract (RGSE) from notoginseng exerts cardioprotection via attenuating ER stress-associated apoptosis. A total of 18 ginsenosides with a concentration of 816.75 mg/g were characterized and quantified in RGSE. Ginsenoside Rh4, Rg5, Rk3, and Rk1 were the most abundant. RGSE at 50 and 100 mg/kg significantly attenuated myocardial infarction and improved cardiac function in acute myocardial infarction (AMI). RGSE also prevented myocardial ischemia-induced oxidative stress and apoptosis in vivo and in vitro. In addition, RGSE decreased the overexpression of GRP78, GRP94 and CHOP, and inhibited the phosphorylation of PERK and IRE1α in ER stress. Further investigation revealed that RGSE effectually inactivated TXNIP/NLRP3 signaling pathway in AMI hearts. These observations suggest a novel role for notoginseng in regulating AMI. |
| first_indexed | 2024-12-24T04:23:40Z |
| format | Article |
| id | doaj.art-24a3e14198584766ae91cd9e042a59e8 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T04:23:40Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-24a3e14198584766ae91cd9e042a59e82022-12-21T17:15:41ZengElsevierJournal of Functional Foods1756-46462015-06-01162027A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditionsLi-Chao Wang0Wen-Song Zhang1Qun Liu2Jia Li3Raphael N. Alolga4Kang Liu5Bao-Lin Liu6Ping Li7Lian-Wen Qi8State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, China; Corresponding author. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Tel.: +86 25 83271379; fax: +86 25 83271379.State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing 210009, China; Corresponding author. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Tel.: +86 25 83271379; fax: +86 25 83271379.Panax notoginseng possesses therapeutic potential for cardiovascular disorders. Endoplasmic reticulum (ER) stress plays a critical role in cardiovascular disease. Here we show that a rare ginsenoside-standardized extract (RGSE) from notoginseng exerts cardioprotection via attenuating ER stress-associated apoptosis. A total of 18 ginsenosides with a concentration of 816.75 mg/g were characterized and quantified in RGSE. Ginsenoside Rh4, Rg5, Rk3, and Rk1 were the most abundant. RGSE at 50 and 100 mg/kg significantly attenuated myocardial infarction and improved cardiac function in acute myocardial infarction (AMI). RGSE also prevented myocardial ischemia-induced oxidative stress and apoptosis in vivo and in vitro. In addition, RGSE decreased the overexpression of GRP78, GRP94 and CHOP, and inhibited the phosphorylation of PERK and IRE1α in ER stress. Further investigation revealed that RGSE effectually inactivated TXNIP/NLRP3 signaling pathway in AMI hearts. These observations suggest a novel role for notoginseng in regulating AMI.http://www.sciencedirect.com/science/article/pii/S1756464615001917Acute myocardial infarctionApoptosisCardioprotectionEndoplasmic reticulum stressRare ginsenoside-standardized extractSteamed Panax notoginseng |
| spellingShingle | Li-Chao Wang Wen-Song Zhang Qun Liu Jia Li Raphael N. Alolga Kang Liu Bao-Lin Liu Ping Li Lian-Wen Qi A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions Journal of Functional Foods Acute myocardial infarction Apoptosis Cardioprotection Endoplasmic reticulum stress Rare ginsenoside-standardized extract Steamed Panax notoginseng |
| title | A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions |
| title_full | A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions |
| title_fullStr | A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions |
| title_full_unstemmed | A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions |
| title_short | A standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions |
| title_sort | standardized notoginseng extract exerts cardioprotection by attenuating apoptosis under endoplasmic reticulum stress conditions |
| topic | Acute myocardial infarction Apoptosis Cardioprotection Endoplasmic reticulum stress Rare ginsenoside-standardized extract Steamed Panax notoginseng |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615001917 |
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