Metabolites identification of oil palm roots infected with Ganoderma boninense using GC–MS-based metabolomics
An approach to metabolomics profiling of non-infected and Ganoderma boninense (G. boninsense) infected oil palm roots crude extracts that utilize gas chromatography-mass spectrometry (GC–MS) and multivariate statistics of principal component analysis (PCA) have been tested. This combination has prov...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535220301805 |
| _version_ | 1818498483681755136 |
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| author | Azizul Isha Nor Azah Yusof Khozirah Shaari Rosiah Osman Siti Nor Akmar Abdullah Mui-Yun Wong |
| author_facet | Azizul Isha Nor Azah Yusof Khozirah Shaari Rosiah Osman Siti Nor Akmar Abdullah Mui-Yun Wong |
| author_sort | Azizul Isha |
| collection | DOAJ |
| description | An approach to metabolomics profiling of non-infected and Ganoderma boninense (G. boninsense) infected oil palm roots crude extracts that utilize gas chromatography-mass spectrometry (GC–MS) and multivariate statistics of principal component analysis (PCA) have been tested. This combination has provided a rapid approach in investigating the changes in the metabolite variations of non-infected and infected oil palm roots at 14 and 30 days post-infection. The extracts were prepared by using 80% (v/v) of methanol. In identifying the metabolites responsible for each differentiation, PCA model was generated in loading bi-plot. Dimethyl benzene-1,4-dicarboxylate, methyl 3-(3,5-ditert-butyl-4-hydroxyphenyl)propanoate, ergost-5-en-3-ol, (3β), stigmast-5-en-3-ol, (3β), stigmasterol, methyl hexadecanoate, methyl (9Z,12Z)-octadeca-9,12-dienoate, methyl octadecanoate, 2-(hydroxymethyl)-2-nitropropane-1,3-diol, methyl (Z)-octadec-6-enoate and (E)-icos-5-ene were found more abundant in G. boninense infected roots than in non-infected roots. Steroidal compounds and fatty acid derivatives which has been determined in the non-infected and G. boninense infected roots regulate a variety of responses to the G. boninense. The abundant of these metabolites in G. boninense infected roots are due to the crucial roles in pathogen defence. |
| first_indexed | 2024-12-10T20:15:48Z |
| format | Article |
| id | doaj.art-24a42962cbd2416ab5137776a5bcd1d2 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-10T20:15:48Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-24a42962cbd2416ab5137776a5bcd1d22022-12-22T01:35:12ZengElsevierArabian Journal of Chemistry1878-53522020-07-0113761916200Metabolites identification of oil palm roots infected with Ganoderma boninense using GC–MS-based metabolomicsAzizul Isha0Nor Azah Yusof1Khozirah Shaari2Rosiah Osman3Siti Nor Akmar Abdullah4Mui-Yun Wong5Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Corresponding authors at: Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia (A. Isha); Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia (N.A. Yusof).Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Corresponding authors at: Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia (A. Isha); Functional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia (N.A. Yusof).Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaFunctional Devices Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaInstitute of Plantation Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaInstitute of Plantation Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaAn approach to metabolomics profiling of non-infected and Ganoderma boninense (G. boninsense) infected oil palm roots crude extracts that utilize gas chromatography-mass spectrometry (GC–MS) and multivariate statistics of principal component analysis (PCA) have been tested. This combination has provided a rapid approach in investigating the changes in the metabolite variations of non-infected and infected oil palm roots at 14 and 30 days post-infection. The extracts were prepared by using 80% (v/v) of methanol. In identifying the metabolites responsible for each differentiation, PCA model was generated in loading bi-plot. Dimethyl benzene-1,4-dicarboxylate, methyl 3-(3,5-ditert-butyl-4-hydroxyphenyl)propanoate, ergost-5-en-3-ol, (3β), stigmast-5-en-3-ol, (3β), stigmasterol, methyl hexadecanoate, methyl (9Z,12Z)-octadeca-9,12-dienoate, methyl octadecanoate, 2-(hydroxymethyl)-2-nitropropane-1,3-diol, methyl (Z)-octadec-6-enoate and (E)-icos-5-ene were found more abundant in G. boninense infected roots than in non-infected roots. Steroidal compounds and fatty acid derivatives which has been determined in the non-infected and G. boninense infected roots regulate a variety of responses to the G. boninense. The abundant of these metabolites in G. boninense infected roots are due to the crucial roles in pathogen defence.http://www.sciencedirect.com/science/article/pii/S1878535220301805Ganoderma boninenseGC–MSMetabolomicsOil palm rootsPrincipal component analysis |
| spellingShingle | Azizul Isha Nor Azah Yusof Khozirah Shaari Rosiah Osman Siti Nor Akmar Abdullah Mui-Yun Wong Metabolites identification of oil palm roots infected with Ganoderma boninense using GC–MS-based metabolomics Arabian Journal of Chemistry Ganoderma boninense GC–MS Metabolomics Oil palm roots Principal component analysis |
| title | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC–MS-based metabolomics |
| title_full | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC–MS-based metabolomics |
| title_fullStr | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC–MS-based metabolomics |
| title_full_unstemmed | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC–MS-based metabolomics |
| title_short | Metabolites identification of oil palm roots infected with Ganoderma boninense using GC–MS-based metabolomics |
| title_sort | metabolites identification of oil palm roots infected with ganoderma boninense using gc ms based metabolomics |
| topic | Ganoderma boninense GC–MS Metabolomics Oil palm roots Principal component analysis |
| url | http://www.sciencedirect.com/science/article/pii/S1878535220301805 |
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