In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis Model
Though the exact etiology of rheumatoid arthritis (RA) is unknown, the contribution of immune cells in the disease process is completely acknowledged. T helper (Th) 1 and Th17-related cytokines are required for the disease development and progression, while Th2 and regulatory T cells (Tregs)-derived...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-05-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2548 |
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| author | Peyman Bemani Zahra Amirghofran Eskandar Kamali-Sarvestani |
| author_facet | Peyman Bemani Zahra Amirghofran Eskandar Kamali-Sarvestani |
| author_sort | Peyman Bemani |
| collection | DOAJ |
| description | Though the exact etiology of rheumatoid arthritis (RA) is unknown, the contribution of immune cells in the disease process is completely acknowledged. T helper (Th) 1 and Th17-related cytokines are required for the disease development and progression, while Th2 and regulatory T cells (Tregs)-derived cytokines are protective. Studies have shown that sodium benzoate (NaB) can switch the balance of Th cell subsets toward Th2 and Tregs. The present study aimed to evaluate the possible effects of NaB on the expression of CD4+T cells-related cytokines and transcription factors in splenocytes derived from an animal model of RA, adjuvant-induced arthritis (AIA).
AIA was induced in rats by injection of Freund's adjuvant containing mycobacterial antigens (Mtb). Splenocytes were isolated from AIA rats and restimulated ex vivo with Mtb in the presence or absence of NaB for 24 h. To determine the effects of NaB on the expression of T cells-related cytokine and transcription factor genes, real-time PCR was performed.
NaB treatment of Mtb-stimulated splenocytes derived from arthritic rats resulted in significant increases in the gene expressions of Tregs-related cytokines (IL-10 and TGF-β) and Foxp3 transcription factor, and significant decreases in the expression of Th1-related cytokines (TNF-α and IFN-γ) and the T-bet transcription factor. The ratios of Th1/Th2 (IFN-γ/IL-4), Th1/Treg (IFN-γ/TGF-β and IFN-γ/IL-10) and Th17/Treg (IL-17/IL-10 and IL-17/IL-10+TGF-β)-related cytokines were also significantly decreased.
In conclusion, NaB can potentially be considered as a useful therapeutic agent for the treatment of RA and other Th1 and Th17-mediated diseases. |
| first_indexed | 2024-12-11T02:47:00Z |
| format | Article |
| id | doaj.art-24a447c49c154787b0fa2dcfdbee02eb |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-11T02:47:00Z |
| publishDate | 2020-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-24a447c49c154787b0fa2dcfdbee02eb2022-12-22T01:23:23ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492020-05-0119S110.18502/ijaai.v19i(s1.r1).28532548In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis ModelPeyman Bemani0Zahra Amirghofran1Eskandar Kamali-Sarvestani2Department of Immunology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran AND Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Immunology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran AND Autoimmune Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Immunology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran AND Autoimmune Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, IranThough the exact etiology of rheumatoid arthritis (RA) is unknown, the contribution of immune cells in the disease process is completely acknowledged. T helper (Th) 1 and Th17-related cytokines are required for the disease development and progression, while Th2 and regulatory T cells (Tregs)-derived cytokines are protective. Studies have shown that sodium benzoate (NaB) can switch the balance of Th cell subsets toward Th2 and Tregs. The present study aimed to evaluate the possible effects of NaB on the expression of CD4+T cells-related cytokines and transcription factors in splenocytes derived from an animal model of RA, adjuvant-induced arthritis (AIA). AIA was induced in rats by injection of Freund's adjuvant containing mycobacterial antigens (Mtb). Splenocytes were isolated from AIA rats and restimulated ex vivo with Mtb in the presence or absence of NaB for 24 h. To determine the effects of NaB on the expression of T cells-related cytokine and transcription factor genes, real-time PCR was performed. NaB treatment of Mtb-stimulated splenocytes derived from arthritic rats resulted in significant increases in the gene expressions of Tregs-related cytokines (IL-10 and TGF-β) and Foxp3 transcription factor, and significant decreases in the expression of Th1-related cytokines (TNF-α and IFN-γ) and the T-bet transcription factor. The ratios of Th1/Th2 (IFN-γ/IL-4), Th1/Treg (IFN-γ/TGF-β and IFN-γ/IL-10) and Th17/Treg (IL-17/IL-10 and IL-17/IL-10+TGF-β)-related cytokines were also significantly decreased. In conclusion, NaB can potentially be considered as a useful therapeutic agent for the treatment of RA and other Th1 and Th17-mediated diseases.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2548Adjuvant-induced arthritisCD4 T cellsCytokinesSodium benzoateTranscription factors |
| spellingShingle | Peyman Bemani Zahra Amirghofran Eskandar Kamali-Sarvestani In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis Model Iranian Journal of Allergy, Asthma and Immunology Adjuvant-induced arthritis CD4 T cells Cytokines Sodium benzoate Transcription factors |
| title | In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis Model |
| title_full | In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis Model |
| title_fullStr | In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis Model |
| title_full_unstemmed | In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis Model |
| title_short | In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis Model |
| title_sort | in vitro effects of sodium benzoate on the expression of t cells related cytokines and transcription factors in adjuvant induced arthritis model |
| topic | Adjuvant-induced arthritis CD4 T cells Cytokines Sodium benzoate Transcription factors |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2548 |
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