Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer’s Disease Models
Accumulated experimental data strongly suggest that astrocytes play an important role in the pathogenesis of neurodegeneration, including Alzheimer’s disease (AD). The effect of astrocytes on the calcium activity of neuron–astroglia networks in AD modelling was the object of the present study. We ha...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15928 |
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| author | Elena V. Mitroshina Alexander M. Pakhomov Mikhail I. Krivonosov Roman S. Yarkov Maria S. Gavrish Alexey V. Shkirin Mikhail V. Ivanchenko Maria V. Vedunova |
| author_facet | Elena V. Mitroshina Alexander M. Pakhomov Mikhail I. Krivonosov Roman S. Yarkov Maria S. Gavrish Alexey V. Shkirin Mikhail V. Ivanchenko Maria V. Vedunova |
| author_sort | Elena V. Mitroshina |
| collection | DOAJ |
| description | Accumulated experimental data strongly suggest that astrocytes play an important role in the pathogenesis of neurodegeneration, including Alzheimer’s disease (AD). The effect of astrocytes on the calcium activity of neuron–astroglia networks in AD modelling was the object of the present study. We have expanded and improved our approach’s capabilities to analyze calcium activity. We have developed a novel algorithm to construct dynamic directed graphs of both astrocytic and neuronal networks. The proposed algorithm allows us not only to identify functional relationships between cells and determine the presence of network activity, but also to characterize the spread of the calcium signal from cell to cell. Our study showed that Alzheimer’s astrocytes can change the functional pattern of the calcium activity of healthy nerve cells. When healthy nerve cells were cocultivated with astrocytes treated with Aβ42, activation of calcium signaling was found. When healthy nerve cells were cocultivated with 5xFAD astrocytes, inhibition of calcium signaling was observed. In this regard, it seems relevant to further study astrocytic–neuronal interactions as an important factor in the regulation of the functional activity of brain cells during neurodegenerative processes. The approach to the analysis of streaming imaging data developed by the authors is a promising tool for studying the collective calcium dynamics of nerve cells. |
| first_indexed | 2024-03-09T03:32:11Z |
| format | Article |
| id | doaj.art-24a5f5fe368a4074ba564ef46ff83f06 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T03:32:11Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-24a5f5fe368a4074ba564ef46ff83f062023-12-03T14:53:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241592810.3390/ijms232415928Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer’s Disease ModelsElena V. Mitroshina0Alexander M. Pakhomov1Mikhail I. Krivonosov2Roman S. Yarkov3Maria S. Gavrish4Alexey V. Shkirin5Mikhail V. Ivanchenko6Maria V. Vedunova7Department of Neurotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603022, RussiaDepartment of Neurotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603022, RussiaDepartment of Neurotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603022, RussiaDepartment of Neurotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603022, RussiaDepartment of Neurotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603022, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, Vavilova St. 38, Moscow 119991, RussiaDepartment of Applied Mathematics, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603022, RussiaDepartment of Neurotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603022, RussiaAccumulated experimental data strongly suggest that astrocytes play an important role in the pathogenesis of neurodegeneration, including Alzheimer’s disease (AD). The effect of astrocytes on the calcium activity of neuron–astroglia networks in AD modelling was the object of the present study. We have expanded and improved our approach’s capabilities to analyze calcium activity. We have developed a novel algorithm to construct dynamic directed graphs of both astrocytic and neuronal networks. The proposed algorithm allows us not only to identify functional relationships between cells and determine the presence of network activity, but also to characterize the spread of the calcium signal from cell to cell. Our study showed that Alzheimer’s astrocytes can change the functional pattern of the calcium activity of healthy nerve cells. When healthy nerve cells were cocultivated with astrocytes treated with Aβ42, activation of calcium signaling was found. When healthy nerve cells were cocultivated with 5xFAD astrocytes, inhibition of calcium signaling was observed. In this regard, it seems relevant to further study astrocytic–neuronal interactions as an important factor in the regulation of the functional activity of brain cells during neurodegenerative processes. The approach to the analysis of streaming imaging data developed by the authors is a promising tool for studying the collective calcium dynamics of nerve cells.https://www.mdpi.com/1422-0067/23/24/15928astrocytesneuron–astroglia networksAlzheimer’s diseaseβ-amyloidcalcium activity |
| spellingShingle | Elena V. Mitroshina Alexander M. Pakhomov Mikhail I. Krivonosov Roman S. Yarkov Maria S. Gavrish Alexey V. Shkirin Mikhail V. Ivanchenko Maria V. Vedunova Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer’s Disease Models International Journal of Molecular Sciences astrocytes neuron–astroglia networks Alzheimer’s disease β-amyloid calcium activity |
| title | Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer’s Disease Models |
| title_full | Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer’s Disease Models |
| title_fullStr | Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer’s Disease Models |
| title_full_unstemmed | Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer’s Disease Models |
| title_short | Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer’s Disease Models |
| title_sort | novel algorithm of network calcium dynamics analysis for studying the role of astrocytes in neuronal activity in alzheimer s disease models |
| topic | astrocytes neuron–astroglia networks Alzheimer’s disease β-amyloid calcium activity |
| url | https://www.mdpi.com/1422-0067/23/24/15928 |
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