Detection of PIK3/AKT pathway in Moroccan population with triple negative breast cancer
Abstract Background Triple Negative Breast Cancer (TNBC) is an aggressive form of breast cancer, that represents 10–20% of all breast carcinomas and characterized by the lack of a specific cell surface marker compared to other breast cancer subtypes. Due to the absence of molecular markers for TNBC...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | BMC Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12885-018-4811-x |
| _version_ | 1819080921825935360 |
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| author | Farah Jouali Nabila Marchoudi Salwa Talbi Basma Bilal Mohamed El Khasmi Houria Rhaissi Jamal Fekkak |
| author_facet | Farah Jouali Nabila Marchoudi Salwa Talbi Basma Bilal Mohamed El Khasmi Houria Rhaissi Jamal Fekkak |
| author_sort | Farah Jouali |
| collection | DOAJ |
| description | Abstract Background Triple Negative Breast Cancer (TNBC) is an aggressive form of breast cancer, that represents 10–20% of all breast carcinomas and characterized by the lack of a specific cell surface marker compared to other breast cancer subtypes. Due to the absence of molecular markers for TNBC his treatment options remains limited, without proven targeted therapies, which emphasize the need for discovering molecular markers that could be targeted for patient treatment, An important number of TNBC cases harbor aberrations in the phosphoinositide 3-kinase (PI3K) pathway, leading to constitutive activation of the downstream signaling pathway. Among mechanisms of PI3K enhancement, PIK3CA mutations are most frequently (~ 30%) observed, along with protein loss of PTEN and AKT activation by phosphorylation (pAkt). Therefore, we propose to analyze clinocopathologic and molecular characteristics of PI3K/AKT/PTEN pathway in Moroccan triple negative breast cancer patients. Methods We conducted a retrospective study of 39 patients diagnosed with triple negative breast cancer between early 2013 and 2016. In this study, we used the Ion Personal Genome Machine (PGM) and Ion Torrent Ampliseq Cancer panel to sequence hotspot regions from PIK3CA, AKT and PTEN genes to identify genetic mutations in 39 samples of TNBC subtype from Moroccan patients and to correlate the results with clinical-pathologic data. Results All patients were female with a median age of 46 years from (34–65). Most patients have had invasive ductal carcinoma (84.6%) and 69.2% of them were grade III SBR. Among the 39, 9 were right sided tumor patients and the remaining 30 were left-sided. Mutational analysis of PIK3CA gene was achieved in all TNBC patients. PIK3CA hotspot mutations were detected in 5/39 of TNBC (13%), in detail, among these 5 TNBC patients, one harbored mutation in exons 9 and four in exon 20. Conclusion The PI3KCA gene is highly activated and plays a crucial role in the pathogenesis of TNBC more, therefore, may be a potential therapeutic target to improve outcomes in patients. |
| first_indexed | 2024-12-21T19:52:34Z |
| format | Article |
| id | doaj.art-24aa806cdefc4b2295157c7726263b50 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-21T19:52:34Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-24aa806cdefc4b2295157c7726263b502022-12-21T18:52:11ZengBMCBMC Cancer1471-24072018-09-011811610.1186/s12885-018-4811-xDetection of PIK3/AKT pathway in Moroccan population with triple negative breast cancerFarah Jouali0Nabila Marchoudi1Salwa Talbi2Basma Bilal3Mohamed El Khasmi4Houria Rhaissi5Jamal Fekkak6Anoual Laboratory of Radio-Immuno AnalysisAnoual Laboratory of Radio-Immuno AnalysisDepartment of Oncology, Center Hospital University Mohammed VIDepartment of Pathology, Center Hospital University Ibn RochdLaboratory of Pathophysiology and Molecular Genetics, Ben M’Sik Faculty of ScienceLaboratory of Pathophysiology and Molecular Genetics, Ben M’Sik Faculty of ScienceAnoual Laboratory of Radio-Immuno AnalysisAbstract Background Triple Negative Breast Cancer (TNBC) is an aggressive form of breast cancer, that represents 10–20% of all breast carcinomas and characterized by the lack of a specific cell surface marker compared to other breast cancer subtypes. Due to the absence of molecular markers for TNBC his treatment options remains limited, without proven targeted therapies, which emphasize the need for discovering molecular markers that could be targeted for patient treatment, An important number of TNBC cases harbor aberrations in the phosphoinositide 3-kinase (PI3K) pathway, leading to constitutive activation of the downstream signaling pathway. Among mechanisms of PI3K enhancement, PIK3CA mutations are most frequently (~ 30%) observed, along with protein loss of PTEN and AKT activation by phosphorylation (pAkt). Therefore, we propose to analyze clinocopathologic and molecular characteristics of PI3K/AKT/PTEN pathway in Moroccan triple negative breast cancer patients. Methods We conducted a retrospective study of 39 patients diagnosed with triple negative breast cancer between early 2013 and 2016. In this study, we used the Ion Personal Genome Machine (PGM) and Ion Torrent Ampliseq Cancer panel to sequence hotspot regions from PIK3CA, AKT and PTEN genes to identify genetic mutations in 39 samples of TNBC subtype from Moroccan patients and to correlate the results with clinical-pathologic data. Results All patients were female with a median age of 46 years from (34–65). Most patients have had invasive ductal carcinoma (84.6%) and 69.2% of them were grade III SBR. Among the 39, 9 were right sided tumor patients and the remaining 30 were left-sided. Mutational analysis of PIK3CA gene was achieved in all TNBC patients. PIK3CA hotspot mutations were detected in 5/39 of TNBC (13%), in detail, among these 5 TNBC patients, one harbored mutation in exons 9 and four in exon 20. Conclusion The PI3KCA gene is highly activated and plays a crucial role in the pathogenesis of TNBC more, therefore, may be a potential therapeutic target to improve outcomes in patients.http://link.springer.com/article/10.1186/s12885-018-4811-xPIK3CAAKTPTENTriple negative breast cancerPI3K pathway |
| spellingShingle | Farah Jouali Nabila Marchoudi Salwa Talbi Basma Bilal Mohamed El Khasmi Houria Rhaissi Jamal Fekkak Detection of PIK3/AKT pathway in Moroccan population with triple negative breast cancer BMC Cancer PIK3CA AKT PTEN Triple negative breast cancer PI3K pathway |
| title | Detection of PIK3/AKT pathway in Moroccan population with triple negative breast cancer |
| title_full | Detection of PIK3/AKT pathway in Moroccan population with triple negative breast cancer |
| title_fullStr | Detection of PIK3/AKT pathway in Moroccan population with triple negative breast cancer |
| title_full_unstemmed | Detection of PIK3/AKT pathway in Moroccan population with triple negative breast cancer |
| title_short | Detection of PIK3/AKT pathway in Moroccan population with triple negative breast cancer |
| title_sort | detection of pik3 akt pathway in moroccan population with triple negative breast cancer |
| topic | PIK3CA AKT PTEN Triple negative breast cancer PI3K pathway |
| url | http://link.springer.com/article/10.1186/s12885-018-4811-x |
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